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Glycine binding sites of presynaptic NMDA receptors may tonically regulate glutamate release in the rat visual cortex.
J Neurophysiol. 2007 Jan; 97(1):817-23.JN

Abstract

In the CNS, activation of N-methyl-D-aspartate receptor (NMDA-R) glycine binding sites is a prerequisite for activation of postsynaptic NMDA-Rs by the excitatory neurotransmitter glutamate. Here we provide electrophysiological evidence that the glycine binding sites of presynaptic NMDA-Rs regulate glutamate release in layer II/III pyramidal neurons of the rat visual cortex. Specifically, our results reveal that the frequency of miniature excitatory postsynaptic currents is significantly reduced by 7-chloro-kynurenic acid (7-Cl KYNA), a NMDA-R glycine binding site antagonist, and glycine or D-serine reverses this effect. Similar results are obtained when the open-channel NMDA receptor blocker, MK-801, is included in the recording pipette. Our data indicate that the glycine binding site of postsynaptic NMDA-Rs is not saturated. Moreover, they suggest that presynaptic NMDA-Rs are located in layer II/III pyramidal neurons of the rat visual cortex and that the glycine binding site of presynaptic NMDA-Rs tonically regulates glutamate release.

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Authors+Show Affiliations

Li YH
Dept. of Physiology, School of Medicine, Xi'an Jiaotong University, Zhuque Dajie 205, Xi'an, Shaanxi 710061, PR China.
Han TZ
No affiliation info available

MeSH

AnimalsBinding SitesBinding, CompetitiveDizocilpine MaleateExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsGlutamic AcidGlycineKynurenic AcidOrgan Culture TechniquesPresynaptic TerminalsPyramidal CellsRatsRats, Sprague-DawleyReceptors, N-Methyl-D-AspartateSynaptic TransmissionVisual Cortex

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17093111

Citation

Li, Yan-Hai, and Tai-Zhen Han. "Glycine Binding Sites of Presynaptic NMDA Receptors May Tonically Regulate Glutamate Release in the Rat Visual Cortex." Journal of Neurophysiology, vol. 97, no. 1, 2007, pp. 817-23.
Li YH, Han TZ. Glycine binding sites of presynaptic NMDA receptors may tonically regulate glutamate release in the rat visual cortex. J Neurophysiol. 2007;97(1):817-23.
Li, Y. H., & Han, T. Z. (2007). Glycine binding sites of presynaptic NMDA receptors may tonically regulate glutamate release in the rat visual cortex. Journal of Neurophysiology, 97(1), 817-23.
Li YH, Han TZ. Glycine Binding Sites of Presynaptic NMDA Receptors May Tonically Regulate Glutamate Release in the Rat Visual Cortex. J Neurophysiol. 2007;97(1):817-23. PubMed PMID: 17093111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glycine binding sites of presynaptic NMDA receptors may tonically regulate glutamate release in the rat visual cortex. AU - Li,Yan-Hai, AU - Han,Tai-Zhen, Y1 - 2006/11/08/ PY - 2006/11/10/pubmed PY - 2007/3/10/medline PY - 2006/11/10/entrez SP - 817 EP - 23 JF - Journal of neurophysiology JO - J Neurophysiol VL - 97 IS - 1 N2 - In the CNS, activation of N-methyl-D-aspartate receptor (NMDA-R) glycine binding sites is a prerequisite for activation of postsynaptic NMDA-Rs by the excitatory neurotransmitter glutamate. Here we provide electrophysiological evidence that the glycine binding sites of presynaptic NMDA-Rs regulate glutamate release in layer II/III pyramidal neurons of the rat visual cortex. Specifically, our results reveal that the frequency of miniature excitatory postsynaptic currents is significantly reduced by 7-chloro-kynurenic acid (7-Cl KYNA), a NMDA-R glycine binding site antagonist, and glycine or D-serine reverses this effect. Similar results are obtained when the open-channel NMDA receptor blocker, MK-801, is included in the recording pipette. Our data indicate that the glycine binding site of postsynaptic NMDA-Rs is not saturated. Moreover, they suggest that presynaptic NMDA-Rs are located in layer II/III pyramidal neurons of the rat visual cortex and that the glycine binding site of presynaptic NMDA-Rs tonically regulates glutamate release. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/17093111/Glycine_binding_sites_of_presynaptic_NMDA_receptors_may_tonically_regulate_glutamate_release_in_the_rat_visual_cortex_ L2 - https://journals.physiology.org/doi/10.1152/jn.00980.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -