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Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei.
J Neurophysiol 2007; 97(1):901-11JN

Abstract

The deep cerebellar nuclei (DCN) integrate inputs from the brain stem, the inferior olive, and the spinal cord with Purkinje cell output from cerebellar cortex and provide the major output of the cerebellum. Despite their crucial function in motor control and learning, the various populations of neurons in the DCN are poorly defined and characterized. Importantly, differences in electrophysiological properties between glutamatergic and GABAergic cells of the DCN have been largely elusive. Here, we used glutamate decarboxylase (GAD) 67-green fluorescent protein (GFP) knock-in mice to unambiguously identify GABAergic (GAD-positive) and non-GABAergic (GAD-negative, most likely glutamatergic) neurons of the DCN. Morphological analysis of DCN neurons patch-clamped with biocytin-containing electrodes revealed a significant overlap in the distributions of the soma sizes of GAD-positive and GAD-negative cells. Compared with GAD-negative DCN neurons, GAD-positive DCN neurons fire broader action potentials, display stronger frequency accommodation, and do not reach as high firing frequencies during depolarizing current injections. Furthermore, GAD-positive cells display slower spontaneous firing rates and have a more shallow frequency-to-current relationship than the GAD-negative cells but exhibit a longer-lasting rebound depolarization and associated spiking after a transient hyperpolarization. In contrast to the rather homogeneous population of GAD-positive cells, the GAD-negative cells were found to consist of two distinct populations as defined by cell size and electrophysiological features. We conclude that GABAergic DCN neurons are specialized to convey phasic spike rate information, whereas tonic spike rate is more faithfully relayed by the large non-GABAergic cells.

Authors+Show Affiliations

Laboratory for Neuronal Circuit Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17093116

Citation

Uusisaari, Marylka, et al. "Morphological and Electrophysiological Properties of GABAergic and non-GABAergic Cells in the Deep Cerebellar Nuclei." Journal of Neurophysiology, vol. 97, no. 1, 2007, pp. 901-11.
Uusisaari M, Obata K, Knöpfel T. Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei. J Neurophysiol. 2007;97(1):901-11.
Uusisaari, M., Obata, K., & Knöpfel, T. (2007). Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei. Journal of Neurophysiology, 97(1), pp. 901-11.
Uusisaari M, Obata K, Knöpfel T. Morphological and Electrophysiological Properties of GABAergic and non-GABAergic Cells in the Deep Cerebellar Nuclei. J Neurophysiol. 2007;97(1):901-11. PubMed PMID: 17093116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei. AU - Uusisaari,Marylka, AU - Obata,Kunihiko, AU - Knöpfel,Thomas, Y1 - 2006/11/08/ PY - 2006/11/10/pubmed PY - 2007/3/10/medline PY - 2006/11/10/entrez SP - 901 EP - 11 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 97 IS - 1 N2 - The deep cerebellar nuclei (DCN) integrate inputs from the brain stem, the inferior olive, and the spinal cord with Purkinje cell output from cerebellar cortex and provide the major output of the cerebellum. Despite their crucial function in motor control and learning, the various populations of neurons in the DCN are poorly defined and characterized. Importantly, differences in electrophysiological properties between glutamatergic and GABAergic cells of the DCN have been largely elusive. Here, we used glutamate decarboxylase (GAD) 67-green fluorescent protein (GFP) knock-in mice to unambiguously identify GABAergic (GAD-positive) and non-GABAergic (GAD-negative, most likely glutamatergic) neurons of the DCN. Morphological analysis of DCN neurons patch-clamped with biocytin-containing electrodes revealed a significant overlap in the distributions of the soma sizes of GAD-positive and GAD-negative cells. Compared with GAD-negative DCN neurons, GAD-positive DCN neurons fire broader action potentials, display stronger frequency accommodation, and do not reach as high firing frequencies during depolarizing current injections. Furthermore, GAD-positive cells display slower spontaneous firing rates and have a more shallow frequency-to-current relationship than the GAD-negative cells but exhibit a longer-lasting rebound depolarization and associated spiking after a transient hyperpolarization. In contrast to the rather homogeneous population of GAD-positive cells, the GAD-negative cells were found to consist of two distinct populations as defined by cell size and electrophysiological features. We conclude that GABAergic DCN neurons are specialized to convey phasic spike rate information, whereas tonic spike rate is more faithfully relayed by the large non-GABAergic cells. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/17093116/Morphological_and_electrophysiological_properties_of_GABAergic_and_non_GABAergic_cells_in_the_deep_cerebellar_nuclei_ L2 - http://www.physiology.org/doi/full/10.1152/jn.00974.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -