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Synergistic effect of supplemental enteral nutrients and exogenous glucagon-like peptide 2 on intestinal adaptation in a rat model of short bowel syndrome.
Am J Clin Nutr. 2006 Nov; 84(5):1142-50.AJ

Abstract

BACKGROUND

Short bowel syndrome (SBS) can lead to intestinal failure and require total or supplemental parenteral nutrition (TPN or PN, respectively). Glucagon-like peptide 2 (GLP-2) is a nutrient-dependent, proglucagon-derived gut hormone that stimulates intestinal adaptation.

OBJECTIVE

Our objective was to determine whether supplemental enteral nutrients (SEN) modulate the intestinotrophic response to a low dose of GLP-2 coinfused with PN in a rat model of SBS (60% jejunoileal resection plus cecectomy).

DESIGN

Rats were randomly assigned to 8 treatments by using a 2 x 2 x 2 factorial design and maintained with either TPN or PN for 7 d. The 3 main treatment effects were the following: transection or resection (TPN alone), +/- SEN (days 4-6), and +/- GLP-2 (100 mug . kg body wt(-1) . d(-1)).

RESULTS

The treatments induced differential growth of duodenal and jejunal mucosa. Significant differences in villus height, crypt depth, dry mass, and concentrations of protein and DNA were observed between the treatments and TPN alone (SEN: 15-59% increase; GLP-2: 14-84% increase; and SEN + GLP-2: 63-160% increase). Plasma concentrations of bioactive GLP-2 were significantly greater with GLP-2 infusion (TPN alone: 25 +/- 9 pmol/L; SEN: 29 +/- 10 pmol/L; GLP-2: 59 +/- 31 pmol/L; SEN + GLP-2: 246 +/- 40 pmol/L) and correlated with mucosal growth. Jejunal sucrase activity (in U/cm) was significantly greater with SEN than without SEN. SEN + GLP-2 induced dramatic mucosal growth and greater plasma concentration of GLP-2 (SEN x GLP-2 interaction, P < 0.0001). Resection significantly increased expression of proglucagon mRNA in colon.

CONCLUSIONS

Combination treatment with SEN and GLP-2 induced a synergistic response resulting in greater mucosal cellularity and digestive capacity in parenterally fed rats with SBS. This shows that SEN improve the intestinotrophic response to exogenous GLP-2, possibly by stimulating enterocyte proliferation and differentiation.

Authors+Show Affiliations

Liu X
Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Nelson DW
No affiliation info available
Holst JJ
No affiliation info available
Ney DM
No affiliation info available

MeSH

Adaptation, PhysiologicalAnimalsCell DivisionDisease Models, AnimalDrug SynergismDrug Therapy, CombinationEnteral NutritionGlucagon-Like Peptide 2Intestinal MucosaIntestine, SmallMaleParenteral Nutrition, TotalRNA, MessengerRandom AllocationRatsRats, Sprague-DawleyShort Bowel SyndromeSucraseTreatment Outcome

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17093168

Citation

Liu, Xiaowen, et al. "Synergistic Effect of Supplemental Enteral Nutrients and Exogenous Glucagon-like Peptide 2 On Intestinal Adaptation in a Rat Model of Short Bowel Syndrome." The American Journal of Clinical Nutrition, vol. 84, no. 5, 2006, pp. 1142-50.
Liu X, Nelson DW, Holst JJ, et al. Synergistic effect of supplemental enteral nutrients and exogenous glucagon-like peptide 2 on intestinal adaptation in a rat model of short bowel syndrome. Am J Clin Nutr. 2006;84(5):1142-50.
Liu, X., Nelson, D. W., Holst, J. J., & Ney, D. M. (2006). Synergistic effect of supplemental enteral nutrients and exogenous glucagon-like peptide 2 on intestinal adaptation in a rat model of short bowel syndrome. The American Journal of Clinical Nutrition, 84(5), 1142-50.
Liu X, et al. Synergistic Effect of Supplemental Enteral Nutrients and Exogenous Glucagon-like Peptide 2 On Intestinal Adaptation in a Rat Model of Short Bowel Syndrome. Am J Clin Nutr. 2006;84(5):1142-50. PubMed PMID: 17093168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic effect of supplemental enteral nutrients and exogenous glucagon-like peptide 2 on intestinal adaptation in a rat model of short bowel syndrome. AU - Liu,Xiaowen, AU - Nelson,David W, AU - Holst,Jens J, AU - Ney,Denise M, PY - 2006/11/10/pubmed PY - 2006/12/15/medline PY - 2006/11/10/entrez SP - 1142 EP - 50 JF - The American journal of clinical nutrition JO - Am J Clin Nutr VL - 84 IS - 5 N2 - BACKGROUND: Short bowel syndrome (SBS) can lead to intestinal failure and require total or supplemental parenteral nutrition (TPN or PN, respectively). Glucagon-like peptide 2 (GLP-2) is a nutrient-dependent, proglucagon-derived gut hormone that stimulates intestinal adaptation. OBJECTIVE: Our objective was to determine whether supplemental enteral nutrients (SEN) modulate the intestinotrophic response to a low dose of GLP-2 coinfused with PN in a rat model of SBS (60% jejunoileal resection plus cecectomy). DESIGN: Rats were randomly assigned to 8 treatments by using a 2 x 2 x 2 factorial design and maintained with either TPN or PN for 7 d. The 3 main treatment effects were the following: transection or resection (TPN alone), +/- SEN (days 4-6), and +/- GLP-2 (100 mug . kg body wt(-1) . d(-1)). RESULTS: The treatments induced differential growth of duodenal and jejunal mucosa. Significant differences in villus height, crypt depth, dry mass, and concentrations of protein and DNA were observed between the treatments and TPN alone (SEN: 15-59% increase; GLP-2: 14-84% increase; and SEN + GLP-2: 63-160% increase). Plasma concentrations of bioactive GLP-2 were significantly greater with GLP-2 infusion (TPN alone: 25 +/- 9 pmol/L; SEN: 29 +/- 10 pmol/L; GLP-2: 59 +/- 31 pmol/L; SEN + GLP-2: 246 +/- 40 pmol/L) and correlated with mucosal growth. Jejunal sucrase activity (in U/cm) was significantly greater with SEN than without SEN. SEN + GLP-2 induced dramatic mucosal growth and greater plasma concentration of GLP-2 (SEN x GLP-2 interaction, P < 0.0001). Resection significantly increased expression of proglucagon mRNA in colon. CONCLUSIONS: Combination treatment with SEN and GLP-2 induced a synergistic response resulting in greater mucosal cellularity and digestive capacity in parenterally fed rats with SBS. This shows that SEN improve the intestinotrophic response to exogenous GLP-2, possibly by stimulating enterocyte proliferation and differentiation. SN - 0002-9165 UR - https://www.unboundmedicine.com/medline/citation/17093168/Synergistic_effect_of_supplemental_enteral_nutrients_and_exogenous_glucagon_like_peptide_2_on_intestinal_adaptation_in_a_rat_model_of_short_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -