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Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture.
EMBO J. 2006 Nov 29; 25(23):5539-48.EJ

Abstract

Rad51, Rad52, and RPA play central roles in homologous DNA recombination. Rad51 mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 has two distinct activities: to recruit Rad51 onto single-strand (ss)DNA that is complexed with the ssDNA-binding protein, RPA, and to anneal complementary ssDNA complexed with RPA. Here, we report that Rad52 promotes annealing of the ssDNA strand that is displaced by DNA strand exchange by Rad51 and RPA, to a second ssDNA strand. An RPA that is recombination-deficient (RPA(rfa1-t11)) failed to support annealing, explaining its in vivo phenotype. Escherichia coli RecO and SSB proteins, which are functional homologues of Rad52 and RPA, also facilitated the same reaction, demonstrating its conserved nature. We also demonstrate that the two activities of Rad52, recruiting Rad51 and annealing DNA, are coordinated in DNA strand exchange and second ssDNA capture.

Authors+Show Affiliations

Department of Biological Sciences, Ohio University, Athens, OH 45701, USA. sugiyama@ohio.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17093500

Citation

Sugiyama, Tomohiko, et al. "Rad52-mediated DNA Annealing After Rad51-mediated DNA Strand Exchange Promotes Second ssDNA Capture." The EMBO Journal, vol. 25, no. 23, 2006, pp. 5539-48.
Sugiyama T, Kantake N, Wu Y, et al. Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture. EMBO J. 2006;25(23):5539-48.
Sugiyama, T., Kantake, N., Wu, Y., & Kowalczykowski, S. C. (2006). Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture. The EMBO Journal, 25(23), 5539-48.
Sugiyama T, et al. Rad52-mediated DNA Annealing After Rad51-mediated DNA Strand Exchange Promotes Second ssDNA Capture. EMBO J. 2006 Nov 29;25(23):5539-48. PubMed PMID: 17093500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture. AU - Sugiyama,Tomohiko, AU - Kantake,Noriko, AU - Wu,Yun, AU - Kowalczykowski,Stephen C, Y1 - 2006/11/09/ PY - 2006/05/17/received PY - 2006/10/10/accepted PY - 2006/11/10/pubmed PY - 2007/1/27/medline PY - 2006/11/10/entrez SP - 5539 EP - 48 JF - The EMBO journal JO - EMBO J VL - 25 IS - 23 N2 - Rad51, Rad52, and RPA play central roles in homologous DNA recombination. Rad51 mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 has two distinct activities: to recruit Rad51 onto single-strand (ss)DNA that is complexed with the ssDNA-binding protein, RPA, and to anneal complementary ssDNA complexed with RPA. Here, we report that Rad52 promotes annealing of the ssDNA strand that is displaced by DNA strand exchange by Rad51 and RPA, to a second ssDNA strand. An RPA that is recombination-deficient (RPA(rfa1-t11)) failed to support annealing, explaining its in vivo phenotype. Escherichia coli RecO and SSB proteins, which are functional homologues of Rad52 and RPA, also facilitated the same reaction, demonstrating its conserved nature. We also demonstrate that the two activities of Rad52, recruiting Rad51 and annealing DNA, are coordinated in DNA strand exchange and second ssDNA capture. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/17093500/Rad52_mediated_DNA_annealing_after_Rad51_mediated_DNA_strand_exchange_promotes_second_ssDNA_capture_ L2 - https://doi.org/10.1038/sj.emboj.7601412 DB - PRIME DP - Unbound Medicine ER -