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[Development of a PCR assay for detecting Schistosoma japonicum-infected Oncomelania hupensis].

Abstract

OBJECTIVE

To establish a sensitive and specific PCR assay for detecting Schistosoma japonicum-infected Oncomelania hupensis.

METHODS

Based on 18S-rRNA gene of S. japonicum, a PCR assay for detecting Oncomelania snails infected with S. japonicum was established. The PCR product was sequenced, and the sensitivity, cross-reaction and mass detection experiments of PCR assay were performed.

RESULTS

The location of PCR product for detecting Oncomelania snails infected with S. japonicum was similar to the target DNA, with a length of 469 bp and the same sequence as the target DNA. It was registered in GenBank (Accession No. DQ442999). There was no PCR product for detecting uninfected snail. Experiments showed that the minimum DNA concentration of S. japoncium miracidium to be detected was 40 pg/Rpl. DNA from snail infected with single-tail cercaria could not be detected. The maximum dilution concentration of infected snail DNA pooled with uninfected snail DNA that could be detected was 1:640.

CONCLUSION

The PCR assay for detecting S. japonicum-infected Oncomelania snails shows high sensitivity, specificity and effect of mass detection.

Authors+Show Affiliations

Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, WHO Collaborating Center for Research on Helminthiasis, Hangzhou 310013, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17094624

Citation

Chen, Jun-hu, et al. "[Development of a PCR Assay for Detecting Schistosoma Japonicum-infected Oncomelania Hupensis]." Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, vol. 24, no. 3, 2006, pp. 204-7.
Chen JH, Wen LY, Zhang XZ, et al. [Development of a PCR assay for detecting Schistosoma japonicum-infected Oncomelania hupensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;24(3):204-7.
Chen, J. H., Wen, L. Y., Zhang, X. Z., Zhang, J. F., Yu, L. L., & Hong, L. D. (2006). [Development of a PCR assay for detecting Schistosoma japonicum-infected Oncomelania hupensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, 24(3), pp. 204-7.
Chen JH, et al. [Development of a PCR Assay for Detecting Schistosoma Japonicum-infected Oncomelania Hupensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;24(3):204-7. PubMed PMID: 17094624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Development of a PCR assay for detecting Schistosoma japonicum-infected Oncomelania hupensis]. AU - Chen,Jun-hu, AU - Wen,Li-yong, AU - Zhang,Xu-zhao, AU - Zhang,Jian-feng, AU - Yu,Li-ling, AU - Hong,Lin-di, PY - 2006/11/11/pubmed PY - 2007/10/3/medline PY - 2006/11/11/entrez SP - 204 EP - 7 JF - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases JO - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi VL - 24 IS - 3 N2 - OBJECTIVE: To establish a sensitive and specific PCR assay for detecting Schistosoma japonicum-infected Oncomelania hupensis. METHODS: Based on 18S-rRNA gene of S. japonicum, a PCR assay for detecting Oncomelania snails infected with S. japonicum was established. The PCR product was sequenced, and the sensitivity, cross-reaction and mass detection experiments of PCR assay were performed. RESULTS: The location of PCR product for detecting Oncomelania snails infected with S. japonicum was similar to the target DNA, with a length of 469 bp and the same sequence as the target DNA. It was registered in GenBank (Accession No. DQ442999). There was no PCR product for detecting uninfected snail. Experiments showed that the minimum DNA concentration of S. japoncium miracidium to be detected was 40 pg/Rpl. DNA from snail infected with single-tail cercaria could not be detected. The maximum dilution concentration of infected snail DNA pooled with uninfected snail DNA that could be detected was 1:640. CONCLUSION: The PCR assay for detecting S. japonicum-infected Oncomelania snails shows high sensitivity, specificity and effect of mass detection. SN - 1000-7423 UR - https://www.unboundmedicine.com/medline/citation/17094624/[Development_of_a_PCR_assay_for_detecting_Schistosoma_japonicum_infected_Oncomelania_hupensis]_ L2 - http://www.arb-silva.de/search/show/ssu/pubid/17094624 DB - PRIME DP - Unbound Medicine ER -