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Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris.
Arterioscler Thromb Vasc Biol 2007; 27(1):161-7AT

Abstract

OBJECTIVE

Plasma pentraxin 3 (PTX3) levels are increased in patients with acute myocardial infarction, yet its involvement in unstable angina pectoris (UAP) remains unclear. To critically evaluate the role of PTX3 in UAP, a sensitive and precise measurement of PTX3 concentration is needed.

METHODS AND RESULTS

We established a high sensitive plasma ELISA assay system for the detection of PTX3 using monoclonal antibodies. The lower limit of detection of our ELISA was 0.1 ng/mL, sensitivity far greater than the current commercially available kit. Plasma samples were obtained from 162 consecutive patients treated for hypertension, hyperlipidemia, diabetes mellitus, or cardiovascular disease at a physician's office. PTX3 was not associated with any known coronary risk factors. Additionally, we collected plasma samples from 252 consecutive subjects admitted to a university hospital for coronary artery assessment by coronary angiography. PTX3 was significantly increased in patients in whom coronary intervention was performed. We further analyzed the plasma level of PTX3 in 52 patients with effort angina (EAP) and 16 patients with UAP. Compared with the control group, PTX3 were significantly higher in the UAP group.

CONCLUSIONS

The levels of plasma PTX3 were increased in patients with arterial inflammation, especially UAP. This PTX3 detection system will be useful for the prediction of UAP.

Authors+Show Affiliations

Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro, Tokyo, 153-8904, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17095712

Citation

Inoue, Kenji, et al. "Establishment of a High Sensitivity Plasma Assay for Human Pentraxin3 as a Marker for Unstable Angina Pectoris." Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 27, no. 1, 2007, pp. 161-7.
Inoue K, Sugiyama A, Reid PC, et al. Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris. Arterioscler Thromb Vasc Biol. 2007;27(1):161-7.
Inoue, K., Sugiyama, A., Reid, P. C., Ito, Y., Miyauchi, K., Mukai, S., ... Kodama, T. (2007). Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris. Arteriosclerosis, Thrombosis, and Vascular Biology, 27(1), pp. 161-7.
Inoue K, et al. Establishment of a High Sensitivity Plasma Assay for Human Pentraxin3 as a Marker for Unstable Angina Pectoris. Arterioscler Thromb Vasc Biol. 2007;27(1):161-7. PubMed PMID: 17095712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris. AU - Inoue,Kenji, AU - Sugiyama,Akira, AU - Reid,Patrick C, AU - Ito,Yukio, AU - Miyauchi,Katsumi, AU - Mukai,Sei, AU - Sagara,Mina, AU - Miyamoto,Kyoko, AU - Satoh,Hirokazu, AU - Kohno,Isao, AU - Kurata,Takeshi, AU - Ota,Hiroshi, AU - Mantovani,Alberto, AU - Hamakubo,Takao, AU - Daida,Hiroyuki, AU - Kodama,Tatsuhiko, Y1 - 2006/11/09/ PY - 2006/11/11/pubmed PY - 2007/1/24/medline PY - 2006/11/11/entrez SP - 161 EP - 7 JF - Arteriosclerosis, thrombosis, and vascular biology JO - Arterioscler. Thromb. Vasc. Biol. VL - 27 IS - 1 N2 - OBJECTIVE: Plasma pentraxin 3 (PTX3) levels are increased in patients with acute myocardial infarction, yet its involvement in unstable angina pectoris (UAP) remains unclear. To critically evaluate the role of PTX3 in UAP, a sensitive and precise measurement of PTX3 concentration is needed. METHODS AND RESULTS: We established a high sensitive plasma ELISA assay system for the detection of PTX3 using monoclonal antibodies. The lower limit of detection of our ELISA was 0.1 ng/mL, sensitivity far greater than the current commercially available kit. Plasma samples were obtained from 162 consecutive patients treated for hypertension, hyperlipidemia, diabetes mellitus, or cardiovascular disease at a physician's office. PTX3 was not associated with any known coronary risk factors. Additionally, we collected plasma samples from 252 consecutive subjects admitted to a university hospital for coronary artery assessment by coronary angiography. PTX3 was significantly increased in patients in whom coronary intervention was performed. We further analyzed the plasma level of PTX3 in 52 patients with effort angina (EAP) and 16 patients with UAP. Compared with the control group, PTX3 were significantly higher in the UAP group. CONCLUSIONS: The levels of plasma PTX3 were increased in patients with arterial inflammation, especially UAP. This PTX3 detection system will be useful for the prediction of UAP. SN - 1524-4636 UR - https://www.unboundmedicine.com/medline/citation/17095712/Establishment_of_a_high_sensitivity_plasma_assay_for_human_pentraxin3_as_a_marker_for_unstable_angina_pectoris_ L2 - http://www.ahajournals.org/doi/full/10.1161/01.ATV.0000252126.48375.d5?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -