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Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine.
Forensic Sci Int. 2007 Aug 24; 171(1):44-51.FS

Abstract

A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2>0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE<12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases.

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Authors+Show Affiliations

Concheiro M
Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, C/San Francisco s/n, 15782 Santiago de Compostela, Spain.
Simões SM
No affiliation info available
Quintela O
No affiliation info available
de Castro A
No affiliation info available
Dias MJ
No affiliation info available
Cruz A
No affiliation info available
López-Rivadulla M
No affiliation info available

MeSH

AdolescentAdultAmphetaminesFemaleForensic ToxicologyGas Chromatography-Mass SpectrometryHallucinogensHumansMaleSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17097252

Citation

Concheiro, Marta, et al. "Fast LC-MS/MS Method for the Determination of Amphetamine, Methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in Urine." Forensic Science International, vol. 171, no. 1, 2007, pp. 44-51.
Concheiro M, Simões SM, Quintela O, et al. Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine. Forensic Sci Int. 2007;171(1):44-51.
Concheiro, M., Simões, S. M., Quintela, O., de Castro, A., Dias, M. J., Cruz, A., & López-Rivadulla, M. (2007). Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine. Forensic Science International, 171(1), 44-51.
Concheiro M, et al. Fast LC-MS/MS Method for the Determination of Amphetamine, Methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in Urine. Forensic Sci Int. 2007 Aug 24;171(1):44-51. PubMed PMID: 17097252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine. AU - Concheiro,Marta, AU - Simões,Susana Maria dos Santos Sadler, AU - Quintela,Oscar, AU - de Castro,Ana, AU - Dias,Mário João Rodrigues, AU - Cruz,Angelines, AU - López-Rivadulla,Manuel, Y1 - 2006/11/09/ PY - 2006/05/31/received PY - 2006/10/05/revised PY - 2006/10/08/accepted PY - 2006/11/14/pubmed PY - 2007/10/19/medline PY - 2006/11/14/entrez SP - 44 EP - 51 JF - Forensic science international JO - Forensic Sci Int VL - 171 IS - 1 N2 - A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2>0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE<12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/17097252/Fast_LC_MS/MS_method_for_the_determination_of_amphetamine_methamphetamine_MDA_MDMA_MDEA_MBDB_and_PMA_in_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(06)00618-9 DB - PRIME DP - Unbound Medicine ER -