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Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens.
J Clin Microbiol. 1991 Mar; 29(3):645-7.JC

Abstract

Three monoclonal antibody staining kits, from Genetic Systems, Disease Detection International, and Meridian Diagnostics, were compared with calcofluor white for the direct detection of Pneumocystis carinii in respiratory specimens. Of the 150 specimens tested, 23 were found positive for P. carinii by any of the four stains; 13 were bronchoalveolar lavage, 7 were induced sputum and 3 were expectorated sputum specimens. All stains detected the positive bronchoalveolar lavage specimens, the Genetic Systems stain detected six induced sputum specimens, and the other stains each detected four induced sputum specimens. The monoclonal antibody stains detected all three expectorated specimens, while the calcofluor stain detected only one. Overall, the sensitivities of the stains were 96% for Genetic Systems, 87% for both Disease Detection International and Meridian Diagnostics, and 78% for calcofluor.

Authors+Show Affiliations

Department of Pathology, University of California, Irvine, Medical Center, Orange 92668.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1709951

Citation

Stratton, N, et al. "Comparison of Monoclonal Antibody and Calcofluor White Stains for the Detection of Pneumocystis Carinii From Respiratory Specimens." Journal of Clinical Microbiology, vol. 29, no. 3, 1991, pp. 645-7.
Stratton N, Hryniewicki J, Aarnaes SL, et al. Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens. J Clin Microbiol. 1991;29(3):645-7.
Stratton, N., Hryniewicki, J., Aarnaes, S. L., Tan, G., de la Maza, L. M., & Peterson, E. M. (1991). Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens. Journal of Clinical Microbiology, 29(3), 645-7.
Stratton N, et al. Comparison of Monoclonal Antibody and Calcofluor White Stains for the Detection of Pneumocystis Carinii From Respiratory Specimens. J Clin Microbiol. 1991;29(3):645-7. PubMed PMID: 1709951.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens. AU - Stratton,N, AU - Hryniewicki,J, AU - Aarnaes,S L, AU - Tan,G, AU - de la Maza,L M, AU - Peterson,E M, PY - 1991/3/1/pubmed PY - 1991/3/1/medline PY - 1991/3/1/entrez SP - 645 EP - 7 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 29 IS - 3 N2 - Three monoclonal antibody staining kits, from Genetic Systems, Disease Detection International, and Meridian Diagnostics, were compared with calcofluor white for the direct detection of Pneumocystis carinii in respiratory specimens. Of the 150 specimens tested, 23 were found positive for P. carinii by any of the four stains; 13 were bronchoalveolar lavage, 7 were induced sputum and 3 were expectorated sputum specimens. All stains detected the positive bronchoalveolar lavage specimens, the Genetic Systems stain detected six induced sputum specimens, and the other stains each detected four induced sputum specimens. The monoclonal antibody stains detected all three expectorated specimens, while the calcofluor stain detected only one. Overall, the sensitivities of the stains were 96% for Genetic Systems, 87% for both Disease Detection International and Meridian Diagnostics, and 78% for calcofluor. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/1709951/Comparison_of_monoclonal_antibody_and_calcofluor_white_stains_for_the_detection_of_Pneumocystis_carinii_from_respiratory_specimens_ L2 - https://journals.asm.org/doi/10.1128/jcm.29.3.645-647.1991?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -