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Heterogeneity of glycinergic and gabaergic interneurons in the granule cell layer of mouse cerebellum.
J Comp Neurol 2007; 500(1):71-83JC

Abstract

Interneurons of the cerebellum granule cell layer (GCL) form distinct populations. Golgi cells extend dendrites in the molecular layer (ML) and innervate granule cells. In contrast, Lugaro cells have dendrites confined to the GCL but innervate interneurons in the ML, and globular cells have both their dendrites and axons in the ML. The latter cells were described recently and remain poorly characterized. Although several neurochemical markers have been associated selectively with GCL interneurons, it is unclear how they relate to their morphological classification and neurochemical phenotype (glycinergic and/or gamma-aminobutyric acid [GABA]ergic). Here, we performed a detailed characterization of GCL interneurons in mice expressing enhanced green fluorescent protein (GFP) in glycinergic and GABAergic neurons, respectively. By using immunofluorescence for metabotropic glutamate receptor 2 (mGluR2) and neurogranin as markers, we demonstrate the existence of five non-overlapping subsets of Golgi cells: about 65% are glycinergic/GABAergic and co-express both markers. Two small subsets (5-10%) also contain both neurotransmitters but express only mGluR2; they are distinguished by cell body size and location in the GCL. The fourth subset (15%) is GABAergic only and expresses neurogranin. The fifth subset (5%) is glycinergic only and lacks both markers. Thus, the heterogeneity of Golgi cells suggests that they belong to specific functional circuits and are differentially regulated by mGluRs and Ca(2+)-calmodulin-dependent signaling pathways. In contrast to Golgi cells, Lugaro and globular cells are glycinergic/GABAergic and lack mGluR2 and neurogranin. They each represent at least 15% of GCL interneurons and extensively innervate stellate and basket cells, but not Purkinje cells, emphasizing their contribution to inhibitory control of ML interneurons.

Authors+Show Affiliations

Institute of Pharmacology and Toxicology, University of Zurich, CH-8057 Zurich, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17099896

Citation

Simat, Marija, et al. "Heterogeneity of Glycinergic and Gabaergic Interneurons in the Granule Cell Layer of Mouse Cerebellum." The Journal of Comparative Neurology, vol. 500, no. 1, 2007, pp. 71-83.
Simat M, Parpan F, Fritschy JM. Heterogeneity of glycinergic and gabaergic interneurons in the granule cell layer of mouse cerebellum. J Comp Neurol. 2007;500(1):71-83.
Simat, M., Parpan, F., & Fritschy, J. M. (2007). Heterogeneity of glycinergic and gabaergic interneurons in the granule cell layer of mouse cerebellum. The Journal of Comparative Neurology, 500(1), pp. 71-83.
Simat M, Parpan F, Fritschy JM. Heterogeneity of Glycinergic and Gabaergic Interneurons in the Granule Cell Layer of Mouse Cerebellum. J Comp Neurol. 2007 Jan 1;500(1):71-83. PubMed PMID: 17099896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterogeneity of glycinergic and gabaergic interneurons in the granule cell layer of mouse cerebellum. AU - Simat,Marija, AU - Parpan,Franziska, AU - Fritschy,Jean-Marc, PY - 2006/11/14/pubmed PY - 2007/3/3/medline PY - 2006/11/14/entrez SP - 71 EP - 83 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 500 IS - 1 N2 - Interneurons of the cerebellum granule cell layer (GCL) form distinct populations. Golgi cells extend dendrites in the molecular layer (ML) and innervate granule cells. In contrast, Lugaro cells have dendrites confined to the GCL but innervate interneurons in the ML, and globular cells have both their dendrites and axons in the ML. The latter cells were described recently and remain poorly characterized. Although several neurochemical markers have been associated selectively with GCL interneurons, it is unclear how they relate to their morphological classification and neurochemical phenotype (glycinergic and/or gamma-aminobutyric acid [GABA]ergic). Here, we performed a detailed characterization of GCL interneurons in mice expressing enhanced green fluorescent protein (GFP) in glycinergic and GABAergic neurons, respectively. By using immunofluorescence for metabotropic glutamate receptor 2 (mGluR2) and neurogranin as markers, we demonstrate the existence of five non-overlapping subsets of Golgi cells: about 65% are glycinergic/GABAergic and co-express both markers. Two small subsets (5-10%) also contain both neurotransmitters but express only mGluR2; they are distinguished by cell body size and location in the GCL. The fourth subset (15%) is GABAergic only and expresses neurogranin. The fifth subset (5%) is glycinergic only and lacks both markers. Thus, the heterogeneity of Golgi cells suggests that they belong to specific functional circuits and are differentially regulated by mGluRs and Ca(2+)-calmodulin-dependent signaling pathways. In contrast to Golgi cells, Lugaro and globular cells are glycinergic/GABAergic and lack mGluR2 and neurogranin. They each represent at least 15% of GCL interneurons and extensively innervate stellate and basket cells, but not Purkinje cells, emphasizing their contribution to inhibitory control of ML interneurons. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/17099896/Heterogeneity_of_glycinergic_and_gabaergic_interneurons_in_the_granule_cell_layer_of_mouse_cerebellum_ L2 - https://doi.org/10.1002/cne.21142 DB - PRIME DP - Unbound Medicine ER -