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Interleukin 18 maintains a long-standing inflammation in coeliac disease patients.
Clin Exp Immunol. 2006 Dec; 146(3):479-85.CE

Abstract

Dietary gluten induces an early response in the intestine of coeliac disease patients (CD), within a few hours, and this is driven by high levels of proinflammatory cytokines, including IFNgamma and IL-15, as has been thoroughly shown by gluten stimulation of biopsy explants. Our aim was to identify the immune mediators involved in the long-standing inflammation in untreated CD patients at diagnosis. mRNA and protein levels of TNFalpha, IL-12(p35), IL-12(p40), IL-15, IL-18 and IL-23(p19) were quantified in biopsies from active CD patients, CD patients on a gluten-free diet (GFD), healthy controls, and patients with non-CD inflammation and mild histological changes in the intestine. Biopsies from CD patients on a GFD were also stimulated in vitro with gliadin, and protein expression of IL-15 and IL-18 was analysed. Levels of IL-12 and IL-23 mRNA are nearly absent, and TNFalpha levels remain unchanged among different groups. Both the active and inactive forms of IL-18 protein have been found in all samples from active CD, and protein expression was only localized within the crypts. Levels of IL-15 mRNA remain unchanged, and protein expression, localized within the lamina propria, is found in a small number of samples. In vitro stimulation with gluten induces the expression of IL-15 and IL-18. In active CD, the early response following gluten intake characterized by high IFNgamma levels is driven by IL-18, and probably IL-15, and this alternates with periods of long-standing inflammation with moderate IFNgamma levels, maintained by IL-18 alone.

Authors+Show Affiliations

Department of Paediatrics and Immunology, and Institute of Biology and Molecular Genetics (IBGM), Universidad de Valladolid, Valladolid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17100768

Citation

León, A J., et al. "Interleukin 18 Maintains a Long-standing Inflammation in Coeliac Disease Patients." Clinical and Experimental Immunology, vol. 146, no. 3, 2006, pp. 479-85.
León AJ, Garrote JA, Blanco-Quirós A, et al. Interleukin 18 maintains a long-standing inflammation in coeliac disease patients. Clin Exp Immunol. 2006;146(3):479-85.
León, A. J., Garrote, J. A., Blanco-Quirós, A., Calvo, C., Fernández-Salazar, L., Del Villar, A., Barrera, A., & Arranz, E. (2006). Interleukin 18 maintains a long-standing inflammation in coeliac disease patients. Clinical and Experimental Immunology, 146(3), 479-85.
León AJ, et al. Interleukin 18 Maintains a Long-standing Inflammation in Coeliac Disease Patients. Clin Exp Immunol. 2006;146(3):479-85. PubMed PMID: 17100768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interleukin 18 maintains a long-standing inflammation in coeliac disease patients. AU - León,A J, AU - Garrote,J A, AU - Blanco-Quirós,A, AU - Calvo,C, AU - Fernández-Salazar,L, AU - Del Villar,A, AU - Barrera,A, AU - Arranz,E, PY - 2006/11/15/pubmed PY - 2007/2/21/medline PY - 2006/11/15/entrez SP - 479 EP - 85 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 146 IS - 3 N2 - Dietary gluten induces an early response in the intestine of coeliac disease patients (CD), within a few hours, and this is driven by high levels of proinflammatory cytokines, including IFNgamma and IL-15, as has been thoroughly shown by gluten stimulation of biopsy explants. Our aim was to identify the immune mediators involved in the long-standing inflammation in untreated CD patients at diagnosis. mRNA and protein levels of TNFalpha, IL-12(p35), IL-12(p40), IL-15, IL-18 and IL-23(p19) were quantified in biopsies from active CD patients, CD patients on a gluten-free diet (GFD), healthy controls, and patients with non-CD inflammation and mild histological changes in the intestine. Biopsies from CD patients on a GFD were also stimulated in vitro with gliadin, and protein expression of IL-15 and IL-18 was analysed. Levels of IL-12 and IL-23 mRNA are nearly absent, and TNFalpha levels remain unchanged among different groups. Both the active and inactive forms of IL-18 protein have been found in all samples from active CD, and protein expression was only localized within the crypts. Levels of IL-15 mRNA remain unchanged, and protein expression, localized within the lamina propria, is found in a small number of samples. In vitro stimulation with gluten induces the expression of IL-15 and IL-18. In active CD, the early response following gluten intake characterized by high IFNgamma levels is driven by IL-18, and probably IL-15, and this alternates with periods of long-standing inflammation with moderate IFNgamma levels, maintained by IL-18 alone. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/17100768/Interleukin_18_maintains_a_long_standing_inflammation_in_coeliac_disease_patients_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0009-9104&date=2006&volume=146&issue=3&spage=479 DB - PRIME DP - Unbound Medicine ER -