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Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles.
Eur J Pharm Biopharm. 2007 Jan; 65(1):10-7.EJ

Abstract

The aim of the present study was to probe the structural integrity of insulin after being entrapped into chitosan/alginate nanoparticles produced by ionotropic polyelectrolyte pre-gelation. By manipulating the alginate:chitosan mass ratio and the pH during nanoparticle production, desired nanoparticles with a mean size of 850 (+/-88)nm and insulin association efficiency of 81 (+/-2)% were obtained. Insulin secondary structure was assessed by Fourier transform infrared (FTIR) and circular dichroism (CD) after entrapment into nanoparticles and after release from the particles under gastrointestinal simulated conditions. FTIR second-derivative spectra and area-overlap compared to an insulin standard confirmed that no significant conformational changes of insulin occurred in terms of alpha-helix and beta-sheet content. Far-UV-CD spectra corroborated the preservation of insulin structure during the nanoparticle production procedure. The presented nanoparticulate system is a promising carrier for insulin oral delivery since it preserves insulin structure and therefore also, potentially, its bioactivity.

Authors+Show Affiliations

Department of Pharmaceutical Technology, Faculty of Pharmacy of the University of Porto, Porto, Portugal. bruno.sarmento@ff.up.ptNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17101268

Citation

Sarmento, B, et al. "Probing Insulin's Secondary Structure After Entrapment Into Alginate/chitosan Nanoparticles." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 65, no. 1, 2007, pp. 10-7.
Sarmento B, Ferreira DC, Jorgensen L, et al. Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles. Eur J Pharm Biopharm. 2007;65(1):10-7.
Sarmento, B., Ferreira, D. C., Jorgensen, L., & van de Weert, M. (2007). Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 65(1), 10-7.
Sarmento B, et al. Probing Insulin's Secondary Structure After Entrapment Into Alginate/chitosan Nanoparticles. Eur J Pharm Biopharm. 2007;65(1):10-7. PubMed PMID: 17101268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles. AU - Sarmento,B, AU - Ferreira,D C, AU - Jorgensen,L, AU - van de Weert,M, Y1 - 2006/09/24/ PY - 2006/02/10/received PY - 2006/09/13/revised PY - 2006/09/18/accepted PY - 2006/11/15/pubmed PY - 2007/2/3/medline PY - 2006/11/15/entrez SP - 10 EP - 7 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 65 IS - 1 N2 - The aim of the present study was to probe the structural integrity of insulin after being entrapped into chitosan/alginate nanoparticles produced by ionotropic polyelectrolyte pre-gelation. By manipulating the alginate:chitosan mass ratio and the pH during nanoparticle production, desired nanoparticles with a mean size of 850 (+/-88)nm and insulin association efficiency of 81 (+/-2)% were obtained. Insulin secondary structure was assessed by Fourier transform infrared (FTIR) and circular dichroism (CD) after entrapment into nanoparticles and after release from the particles under gastrointestinal simulated conditions. FTIR second-derivative spectra and area-overlap compared to an insulin standard confirmed that no significant conformational changes of insulin occurred in terms of alpha-helix and beta-sheet content. Far-UV-CD spectra corroborated the preservation of insulin structure during the nanoparticle production procedure. The presented nanoparticulate system is a promising carrier for insulin oral delivery since it preserves insulin structure and therefore also, potentially, its bioactivity. SN - 0939-6411 UR - https://www.unboundmedicine.com/medline/citation/17101268/Probing_insulin's_secondary_structure_after_entrapment_into_alginate/chitosan_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -