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Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth.
J Histochem Cytochem. 2007 Mar; 55(3):247-54.JH

Abstract

During early postnatal development, the myosin heavy chain (MyHC) expression pattern in equine gluteus medius muscle shows adaptation to movement and load,resulting in a decrease in the number of fast MyHC fibers and an increase in the number of slow MyHC fibers. In the present study we correlated the expression of MyHC isoforms to the expression of sarcoplasmic(endo)reticulum Ca2+-ATPase 1 and 2a (SERCA), phospholamban (PLB), calcineurin A (CnA), and calcineurin B (CnB). Gluteus medius muscle biopsies were taken at 0, 2, 4, and 48 weeks and analyzed using immunofluorescence. Both SERCA isoforms and PLB were expressed in almost all fiber types at birth. From 4 weeks of age onward, SERCA1 was exclusively expressed in fast MyHC fibers and SERCA2a and PLB in slow MyHC fibers. At all time points, CnA and CnB proteins were expressed at a basal level in all fibers, but with a higher expression level in MyHC type 1 fibers. From 4 weeks onward, expression of only CnA was also higher in MyHC type 2a and 2ad fibers. We propose a double function of calcineurin in calcium homeostasis and maintenance of slow MyHC fiber type identity. Although equine muscle is already functional at birth, expression patterns of the monitored proteins still show adaptation, depending on the MyHC fiber type.

Authors+Show Affiliations

Department of Pathobiology, Division of Anatomy and Physiology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.158, NL-3508 TD, Utrecht, The Netherlands. c.g.h.eizema@vet.uu.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17101725

Citation

Eizema, Karin, et al. "Differential Expression of Calcineurin and SR Ca2+ Handling Proteins in Equine Muscle Fibers During Early Postnatal Growth." The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, vol. 55, no. 3, 2007, pp. 247-54.
Eizema K, van der Wal DE, van den Burg MM, et al. Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth. J Histochem Cytochem. 2007;55(3):247-54.
Eizema, K., van der Wal, D. E., van den Burg, M. M., de Jonge, H. W., & Everts, M. E. (2007). Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 55(3), 247-54.
Eizema K, et al. Differential Expression of Calcineurin and SR Ca2+ Handling Proteins in Equine Muscle Fibers During Early Postnatal Growth. J Histochem Cytochem. 2007;55(3):247-54. PubMed PMID: 17101725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth. AU - Eizema,Karin, AU - van der Wal,Dianne E, AU - van den Burg,Maarten M M, AU - de Jonge,Henriëtte W, AU - Everts,Maria E, Y1 - 2006/11/13/ PY - 2006/11/15/pubmed PY - 2007/3/21/medline PY - 2006/11/15/entrez SP - 247 EP - 54 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JO - J Histochem Cytochem VL - 55 IS - 3 N2 - During early postnatal development, the myosin heavy chain (MyHC) expression pattern in equine gluteus medius muscle shows adaptation to movement and load,resulting in a decrease in the number of fast MyHC fibers and an increase in the number of slow MyHC fibers. In the present study we correlated the expression of MyHC isoforms to the expression of sarcoplasmic(endo)reticulum Ca2+-ATPase 1 and 2a (SERCA), phospholamban (PLB), calcineurin A (CnA), and calcineurin B (CnB). Gluteus medius muscle biopsies were taken at 0, 2, 4, and 48 weeks and analyzed using immunofluorescence. Both SERCA isoforms and PLB were expressed in almost all fiber types at birth. From 4 weeks of age onward, SERCA1 was exclusively expressed in fast MyHC fibers and SERCA2a and PLB in slow MyHC fibers. At all time points, CnA and CnB proteins were expressed at a basal level in all fibers, but with a higher expression level in MyHC type 1 fibers. From 4 weeks onward, expression of only CnA was also higher in MyHC type 2a and 2ad fibers. We propose a double function of calcineurin in calcium homeostasis and maintenance of slow MyHC fiber type identity. Although equine muscle is already functional at birth, expression patterns of the monitored proteins still show adaptation, depending on the MyHC fiber type. SN - 0022-1554 UR - https://www.unboundmedicine.com/medline/citation/17101725/Differential_expression_of_calcineurin_and_SR_Ca2+_handling_proteins_in_equine_muscle_fibers_during_early_postnatal_growth_ L2 - https://journals.sagepub.com/doi/10.1369/jhc.6A7039.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -