TY - JOUR T1 - Quantitation of buclizine hydrochloride in pharmaceutical formulations and human serum by RP-HPLC. AU - Arayne,M Saeed, AU - Sultana,Najma, AU - Siddiqui,Farhan Ahmed, PY - 2006/11/16/pubmed PY - 2007/1/25/medline PY - 2006/11/16/entrez SP - 326 EP - 9 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 19 IS - 4 N2 - An isocratic reversed phase high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 230 nm has been developed for the determination of buclizine hydrochloride in human serum and dosage formulation. Methylparaben was successfully used as an internal standard. Good chromatographic separation between buclizine and internal standard peaks was achieved by using a stainless steel analytical column Nucleosil, C18 (10 microm, 25 cm x 0.46 cm). The system was operated at room temperature using a mobile phase consisting of acetonitrile-water (1:1) (pH 2.6) with phosphoric acid 85% at a flow rate of 2 ml/min. The calibration curve for buclizine hydrochloride in human serum was linear over the tested concentration range of 10, 3, 1.5, 0.5, 0.15, 0.05, and 0.025 microg/ml with a correlation coefficient of 0.9999. The intra- and inter-run precision and accuracy results were 98.07 to 100.34. The proposed method was validated for selectivity, linearity, accuracy, and precision. The method was found to be suitable for the quality control of buclizine hydrochloride in bulk drug as well as in human serum. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/17105713/Quantitation_of_buclizine_hydrochloride_in_pharmaceutical_formulations_and_human_serum_by_RP_HPLC_ DB - PRIME DP - Unbound Medicine ER -