Tags

Type your tag names separated by a space and hit enter

The effect of nitric oxide inhibition and temporal expression patterns of the mRNA and protein products of nitric oxide synthase genes during in vitro development of bovine pre-implantation embryos.
Reprod Domest Anim. 2006 Dec; 41(6):501-9.RD

Abstract

This study was conducted to determine the effect of Nitric oxide (NO) inhibition in bovine in vitro development and expression analysis of the three Nitric oxide synthase (NOS) isoforms: endothelial (eNOS), neuronal (nNOS) and inducible (iNOS), mRNA and protein in bovine oocytes and embryos. Selective inhibitor of NOS, N-omega-nitro-l-arginine methyl ester (l-NAME) was applied at different doses (0, 0.1, 1 and 10 mm) in maturation (experiment 1A), culture medium (experiment 1B) and in both maturation and culture media (experiment 1C). No significant differences were observed in cleavage and blastocyst rates when oocytes were matured in the presence of l-NAME as long as the inhibitor was omitted during fertilization and culture. However, significantly lower blastocyst rates were observed when l-NAME was present at higher level (10 mm) in culture medium alone and in both maturation and culture media. In experiment 2, mRNA isolated from triplicate pools of oocytes and embryos (n = 15-20) was subjected to quantitative real time reverse transcription polymerase chain reaction to investigate the expression of eNOS, iNOS and nNOS mRNA in normal IVP bovine oocytes and embryos. While eNOS and iNOS transcripts were detected at higher level in oocytes (immature and mature), two-cell and four-cell stage embryos, the nNOS was detected only in immature oocyte, two-cell and morula stages. In experiment 3, eNOS and iNOS protein expression analysis was performed in IVP oocytes and embryos and both proteins were detected in the cytoplasm and the nuclei (weak) of oocytes and embryos. These data provide the first evidence for the role of NO production and the presence of mRNA and protein products of NOS isoforms during bovine embryogenesis.

Authors+Show Affiliations

Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany. tesfaye@itz.uni-bonn.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17107508

Citation

Tesfaye, D, et al. "The Effect of Nitric Oxide Inhibition and Temporal Expression Patterns of the mRNA and Protein Products of Nitric Oxide Synthase Genes During in Vitro Development of Bovine Pre-implantation Embryos." Reproduction in Domestic Animals = Zuchthygiene, vol. 41, no. 6, 2006, pp. 501-9.
Tesfaye D, Kadanga A, Rings F, et al. The effect of nitric oxide inhibition and temporal expression patterns of the mRNA and protein products of nitric oxide synthase genes during in vitro development of bovine pre-implantation embryos. Reprod Domest Anim. 2006;41(6):501-9.
Tesfaye, D., Kadanga, A., Rings, F., Bauch, K., Jennen, D., Nganvongpanit, K., Hölker, M., Tholen, E., Ponsuksili, S., Wimmers, K., Montag, M., Gilles, M., Kirfel, G., Herzog, V., & Schellander, K. (2006). The effect of nitric oxide inhibition and temporal expression patterns of the mRNA and protein products of nitric oxide synthase genes during in vitro development of bovine pre-implantation embryos. Reproduction in Domestic Animals = Zuchthygiene, 41(6), 501-9.
Tesfaye D, et al. The Effect of Nitric Oxide Inhibition and Temporal Expression Patterns of the mRNA and Protein Products of Nitric Oxide Synthase Genes During in Vitro Development of Bovine Pre-implantation Embryos. Reprod Domest Anim. 2006;41(6):501-9. PubMed PMID: 17107508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of nitric oxide inhibition and temporal expression patterns of the mRNA and protein products of nitric oxide synthase genes during in vitro development of bovine pre-implantation embryos. AU - Tesfaye,D, AU - Kadanga,A, AU - Rings,F, AU - Bauch,K, AU - Jennen,D, AU - Nganvongpanit,K, AU - Hölker,M, AU - Tholen,E, AU - Ponsuksili,S, AU - Wimmers,K, AU - Montag,M, AU - Gilles,M, AU - Kirfel,G, AU - Herzog,V, AU - Schellander,K, PY - 2006/11/17/pubmed PY - 2007/1/24/medline PY - 2006/11/17/entrez SP - 501 EP - 9 JF - Reproduction in domestic animals = Zuchthygiene JO - Reprod Domest Anim VL - 41 IS - 6 N2 - This study was conducted to determine the effect of Nitric oxide (NO) inhibition in bovine in vitro development and expression analysis of the three Nitric oxide synthase (NOS) isoforms: endothelial (eNOS), neuronal (nNOS) and inducible (iNOS), mRNA and protein in bovine oocytes and embryos. Selective inhibitor of NOS, N-omega-nitro-l-arginine methyl ester (l-NAME) was applied at different doses (0, 0.1, 1 and 10 mm) in maturation (experiment 1A), culture medium (experiment 1B) and in both maturation and culture media (experiment 1C). No significant differences were observed in cleavage and blastocyst rates when oocytes were matured in the presence of l-NAME as long as the inhibitor was omitted during fertilization and culture. However, significantly lower blastocyst rates were observed when l-NAME was present at higher level (10 mm) in culture medium alone and in both maturation and culture media. In experiment 2, mRNA isolated from triplicate pools of oocytes and embryos (n = 15-20) was subjected to quantitative real time reverse transcription polymerase chain reaction to investigate the expression of eNOS, iNOS and nNOS mRNA in normal IVP bovine oocytes and embryos. While eNOS and iNOS transcripts were detected at higher level in oocytes (immature and mature), two-cell and four-cell stage embryos, the nNOS was detected only in immature oocyte, two-cell and morula stages. In experiment 3, eNOS and iNOS protein expression analysis was performed in IVP oocytes and embryos and both proteins were detected in the cytoplasm and the nuclei (weak) of oocytes and embryos. These data provide the first evidence for the role of NO production and the presence of mRNA and protein products of NOS isoforms during bovine embryogenesis. SN - 0936-6768 UR - https://www.unboundmedicine.com/medline/citation/17107508/The_effect_of_nitric_oxide_inhibition_and_temporal_expression_patterns_of_the_mRNA_and_protein_products_of_nitric_oxide_synthase_genes_during_in_vitro_development_of_bovine_pre_implantation_embryos_ L2 - https://doi.org/10.1111/j.1439-0531.2006.00701.x DB - PRIME DP - Unbound Medicine ER -