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Physical mapping of the blue-grained gene(s) from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat.
Genome. 2006 Sep; 49(9):1109-14.G

Abstract

The original blue-grained wheat, Blue 58, was a substitution line derived from hybridization between common wheat (Triticum aestivum L., 2n=6x=42, ABD) and tall wheatgrass (Thinopyrum ponticum Liu & Wang=Agropyron elongatum, 2n=10x=70, StStEeEbEx), in which one pair of 4D chromosomes was replaced by a pair of alien 4Ag chromosomes (unknown group 4 chromosome from A. ponticum). Blue aleurone might be a useful cytological marker in chromosome engineering and wheat breeding. Cytogenetic analysis showed that blue aleurone was controlled by chromosome 4Ag. GISH analysis proved that the 4Ag was a recombination chromosome; its centromeric and pericentromeric regions were from an E-genome chromosome, but the distal regions of its two arms were from an St-genome chromosome. On its short arm, there was a major pAs1 hybridization band, which was very close to the centromere. GISH and FISH analysis in a set of translocation lines with different seed colors revealed that the gene(s) controlling the blue pigment was located on the long arm of 4Ag. It was physically mapped to the 0.71-0.80 regions (distance measured from the centromere of 4Ag). The blue color is a consequence of dosage of this small chromosome region derived from the St genome. We speculate that the blue-grained gene(s) could activate the anthocyanin biosynthetic pathway of wheat.

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Authors+Show Affiliations

Zheng Q
The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Li B
No affiliation info available
Mu S
No affiliation info available
Zhou H
No affiliation info available
Li Z
No affiliation info available

MeSH

Chromosomes, PlantGenetic MarkersIn Situ HybridizationIn Situ Hybridization, FluorescencePhenotypePhysical Chromosome MappingPigmentationPoaceaeSeedsTranslocation, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17110991

Citation

Zheng, Qi, et al. "Physical Mapping of the Blue-grained Gene(s) From Thinopyrum Ponticum By GISH and FISH in a Set of Translocation Lines With Different Seed Colors in Wheat." Genome, vol. 49, no. 9, 2006, pp. 1109-14.
Zheng Q, Li B, Mu S, et al. Physical mapping of the blue-grained gene(s) from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat. Genome. 2006;49(9):1109-14.
Zheng, Q., Li, B., Mu, S., Zhou, H., & Li, Z. (2006). Physical mapping of the blue-grained gene(s) from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat. Genome, 49(9), 1109-14.
Zheng Q, et al. Physical Mapping of the Blue-grained Gene(s) From Thinopyrum Ponticum By GISH and FISH in a Set of Translocation Lines With Different Seed Colors in Wheat. Genome. 2006;49(9):1109-14. PubMed PMID: 17110991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical mapping of the blue-grained gene(s) from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat. AU - Zheng,Qi, AU - Li,Bin, AU - Mu,Sumei, AU - Zhou,Hanping, AU - Li,Zhensheng, PY - 2006/11/18/pubmed PY - 2007/2/16/medline PY - 2006/11/18/entrez SP - 1109 EP - 14 JF - Genome JO - Genome VL - 49 IS - 9 N2 - The original blue-grained wheat, Blue 58, was a substitution line derived from hybridization between common wheat (Triticum aestivum L., 2n=6x=42, ABD) and tall wheatgrass (Thinopyrum ponticum Liu & Wang=Agropyron elongatum, 2n=10x=70, StStEeEbEx), in which one pair of 4D chromosomes was replaced by a pair of alien 4Ag chromosomes (unknown group 4 chromosome from A. ponticum). Blue aleurone might be a useful cytological marker in chromosome engineering and wheat breeding. Cytogenetic analysis showed that blue aleurone was controlled by chromosome 4Ag. GISH analysis proved that the 4Ag was a recombination chromosome; its centromeric and pericentromeric regions were from an E-genome chromosome, but the distal regions of its two arms were from an St-genome chromosome. On its short arm, there was a major pAs1 hybridization band, which was very close to the centromere. GISH and FISH analysis in a set of translocation lines with different seed colors revealed that the gene(s) controlling the blue pigment was located on the long arm of 4Ag. It was physically mapped to the 0.71-0.80 regions (distance measured from the centromere of 4Ag). The blue color is a consequence of dosage of this small chromosome region derived from the St genome. We speculate that the blue-grained gene(s) could activate the anthocyanin biosynthetic pathway of wheat. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/17110991/Physical_mapping_of_the_blue_grained_gene_s__from_Thinopyrum_ponticum_by_GISH_and_FISH_in_a_set_of_translocation_lines_with_different_seed_colors_in_wheat_ L2 - https://cdnsciencepub.com/doi/10.1139/g06-073?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -