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Green tea polyphenol epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of MMP-2 expression and its activation.
Acta Pharmacol Sin. 2006 Dec; 27(12):1600-7.AP

Abstract

AIM

Epigallocatechin-3-gallate (EGCG) is the major component of green tea polyphenols, whose wide range of biological properties includes anti-fibrogenic activity. Matrix metalloproteinases (MMP) that participate in extracellular matrix degradation are involved in the development of hepatic fibrosis. The present study investigates whether EGCG inhibits activation of the major gelatinase matrix metalloproteinase-2 (MMP-2) in rat hepatic stellate cells (HSC).

METHODS

The expression of MMP-2, tissue inhibitors of metalloproteinases-2 (TIMP-2), and membrane-type 1-MMP (MT1-MMP) was assessed by RT-PCR and Western blot analyses. MMP-2 activity was evaluated by zymography and MT1-MMP activity was assessed by an enzymatic assay. HSC migration was measured by a wound healing assay and cell invasion was performed using Transwell cell culture chambers.

RESULTS

The expression of MMP-2 mRNA and protein in HSC was substantially reduced by EGCG treatment. EGCG treatment also reduced concanavalin A (ConA)-induced activation of secreted MMP-2 and reduced MT1-MMP activity in a dose-dependent manner. In addition, EGCG inhibited either HSC migration or invasion.

CONCLUSION

The abilities of EGCG to suppress MMP-2 activation and HSC invasiveness suggest that EGCG may be useful in the treatment and prevention of hepatic fibrosis.

Authors+Show Affiliations

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17112415

Citation

Zhen, Mao-chuan, et al. "Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Rat Hepatic Stellate Cell Invasion By Inhibition of MMP-2 Expression and Its Activation." Acta Pharmacologica Sinica, vol. 27, no. 12, 2006, pp. 1600-7.
Zhen MC, Huang XH, Wang Q, et al. Green tea polyphenol epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of MMP-2 expression and its activation. Acta Pharmacol Sin. 2006;27(12):1600-7.
Zhen, M. C., Huang, X. H., Wang, Q., Sun, K., Liu, Y. J., Li, W., Zhang, L. J., Cao, L. Q., & Chen, X. L. (2006). Green tea polyphenol epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of MMP-2 expression and its activation. Acta Pharmacologica Sinica, 27(12), 1600-7.
Zhen MC, et al. Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Rat Hepatic Stellate Cell Invasion By Inhibition of MMP-2 Expression and Its Activation. Acta Pharmacol Sin. 2006;27(12):1600-7. PubMed PMID: 17112415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green tea polyphenol epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of MMP-2 expression and its activation. AU - Zhen,Mao-chuan, AU - Huang,Xiao-hui, AU - Wang,Qian, AU - Sun,Kai, AU - Liu,Yun-jian, AU - Li,Wen, AU - Zhang,Long-juan, AU - Cao,Liang-qi, AU - Chen,Xi-ling, PY - 2006/11/23/pubmed PY - 2007/11/7/medline PY - 2006/11/23/entrez SP - 1600 EP - 7 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 27 IS - 12 N2 - AIM: Epigallocatechin-3-gallate (EGCG) is the major component of green tea polyphenols, whose wide range of biological properties includes anti-fibrogenic activity. Matrix metalloproteinases (MMP) that participate in extracellular matrix degradation are involved in the development of hepatic fibrosis. The present study investigates whether EGCG inhibits activation of the major gelatinase matrix metalloproteinase-2 (MMP-2) in rat hepatic stellate cells (HSC). METHODS: The expression of MMP-2, tissue inhibitors of metalloproteinases-2 (TIMP-2), and membrane-type 1-MMP (MT1-MMP) was assessed by RT-PCR and Western blot analyses. MMP-2 activity was evaluated by zymography and MT1-MMP activity was assessed by an enzymatic assay. HSC migration was measured by a wound healing assay and cell invasion was performed using Transwell cell culture chambers. RESULTS: The expression of MMP-2 mRNA and protein in HSC was substantially reduced by EGCG treatment. EGCG treatment also reduced concanavalin A (ConA)-induced activation of secreted MMP-2 and reduced MT1-MMP activity in a dose-dependent manner. In addition, EGCG inhibited either HSC migration or invasion. CONCLUSION: The abilities of EGCG to suppress MMP-2 activation and HSC invasiveness suggest that EGCG may be useful in the treatment and prevention of hepatic fibrosis. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/17112415/Green_tea_polyphenol_epigallocatechin_3_gallate_suppresses_rat_hepatic_stellate_cell_invasion_by_inhibition_of_MMP_2_expression_and_its_activation_ L2 - https://doi.org/10.1111/j.1745-7254.2006.00439.x DB - PRIME DP - Unbound Medicine ER -