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Altered beta-secretase enzyme kinetics and levels of both BACE1 and BACE2 in the Alzheimer's disease brain.
FEBS Lett. 2006 Dec 11; 580(28-29):6550-60.FL

Abstract

beta-Secretase is the rate limiting enzymatic activity in the production of amyloid-beta peptide, the primary component of senile plaque pathology in Alzheimer's disease (AD). This study performed the first comparative analysis of beta-secretase enzyme kinetics in AD and control brain tissue. Results found V(max) values for beta-secretase to be significantly increased, and K(m) values unchanged in AD temporal cortex compared to matched control temporal cortex. The increased V(max) in AD cases, did not correlate with levels of BACE1, and decreased BACE1 and BACE2 levels correlated with the severity of neurofibrillary pathology (I-VI), and synaptic loss in AD. These results indicate that increased V(max) for beta-secretase is a feature of AD pathogenesis and this increase does not correlate directly with levels of BACE1, the principal beta-secretase in brain.

Authors+Show Affiliations

Department of Biochemistry, BioSciences Institute, University College Cork, Cork, Ireland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17113083

Citation

Stockley, John H., et al. "Altered Beta-secretase Enzyme Kinetics and Levels of Both BACE1 and BACE2 in the Alzheimer's Disease Brain." FEBS Letters, vol. 580, no. 28-29, 2006, pp. 6550-60.
Stockley JH, Ravid R, O'Neill C. Altered beta-secretase enzyme kinetics and levels of both BACE1 and BACE2 in the Alzheimer's disease brain. FEBS Lett. 2006;580(28-29):6550-60.
Stockley, J. H., Ravid, R., & O'Neill, C. (2006). Altered beta-secretase enzyme kinetics and levels of both BACE1 and BACE2 in the Alzheimer's disease brain. FEBS Letters, 580(28-29), 6550-60.
Stockley JH, Ravid R, O'Neill C. Altered Beta-secretase Enzyme Kinetics and Levels of Both BACE1 and BACE2 in the Alzheimer's Disease Brain. FEBS Lett. 2006 Dec 11;580(28-29):6550-60. PubMed PMID: 17113083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered beta-secretase enzyme kinetics and levels of both BACE1 and BACE2 in the Alzheimer's disease brain. AU - Stockley,John H, AU - Ravid,Rivka, AU - O'Neill,Cora, Y1 - 2006/11/13/ PY - 2006/06/08/received PY - 2006/09/19/revised PY - 2006/10/31/accepted PY - 2006/11/23/pubmed PY - 2007/1/20/medline PY - 2006/11/23/entrez SP - 6550 EP - 60 JF - FEBS letters JO - FEBS Lett VL - 580 IS - 28-29 N2 - beta-Secretase is the rate limiting enzymatic activity in the production of amyloid-beta peptide, the primary component of senile plaque pathology in Alzheimer's disease (AD). This study performed the first comparative analysis of beta-secretase enzyme kinetics in AD and control brain tissue. Results found V(max) values for beta-secretase to be significantly increased, and K(m) values unchanged in AD temporal cortex compared to matched control temporal cortex. The increased V(max) in AD cases, did not correlate with levels of BACE1, and decreased BACE1 and BACE2 levels correlated with the severity of neurofibrillary pathology (I-VI), and synaptic loss in AD. These results indicate that increased V(max) for beta-secretase is a feature of AD pathogenesis and this increase does not correlate directly with levels of BACE1, the principal beta-secretase in brain. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/17113083/Altered_beta_secretase_enzyme_kinetics_and_levels_of_both_BACE1_and_BACE2_in_the_Alzheimer's_disease_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(06)01309-3 DB - PRIME DP - Unbound Medicine ER -