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Optimization of ciguatoxin extraction method from blood for Pacific ciguatoxin (P-CTX-1).
Toxicon. 2007 Jan; 49(1):100-5.T

Abstract

Ciguatera diagnosis relies on clinical observations associated with a recent consumption of fish. Although needed, direct confirmation of exposure in subjects showing ciguatera disease symptoms is currently unavailable. We previously reported that ciguatoxins were measurable in the blood of mice exposed to extracts of Pacific ciguatoxins isolated from Gambierdiscus polynesiensis, and of Indian Ocean or Caribbean Sea ciguatoxins, isolated from fish. Although highly efficient for extracting spiked purified Caribbean-CTX-1, the methanolic extraction method previously described is found here to yield only 6% recovery of spiked Pacific-CTX-1 (P-CTX-1). We report in this short communication a substantially modified method for ciguatoxin extraction from both dried and fresh blood. With this method, toxin measurement is directly accomplished in acetonitrile deproteinated whole fresh blood or phosphate buffer solution (PBS) eluted dried blood using the N2A cell-based assay. Spike studies using increasing concentrations of purified ciguatoxins reveal linear (r2 above 0.87 for all toxins) and overall efficient toxin recoveries (62%, 96%, and 96% from fresh blood and 75%, 90%, and 74% from dried blood, for C-CTX-1, P-CTX-3C, and P-CTX-1, respectively). Comparative blood matrix analysis for P-CTX-1 recovery shows increased recovery of ciguatoxin activity from whole fresh blood than from dried blood, greater by 20% in P-CTX-1 spiked mice blood and by over 85% in P-CTX-1 exposed mouse blood. In conclusion, both Caribbean and Pacific ciguatoxins can be readily extracted from blood using this modified method; however, in the case of P-CTX-1 we find that fresh blood is optimal.

Authors+Show Affiliations

Marine Biotoxins Program, Center for Coastal Environmental Health and Biomolecular Research, NOAA-National Ocean Service, Charleston, SC 29412, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17113119

Citation

Bottein Dechraoui, Marie-Yasmine, et al. "Optimization of Ciguatoxin Extraction Method From Blood for Pacific Ciguatoxin (P-CTX-1)." Toxicon : Official Journal of the International Society On Toxinology, vol. 49, no. 1, 2007, pp. 100-5.
Bottein Dechraoui MY, Wang Z, Ramsdell JS. Optimization of ciguatoxin extraction method from blood for Pacific ciguatoxin (P-CTX-1). Toxicon. 2007;49(1):100-5.
Bottein Dechraoui, M. Y., Wang, Z., & Ramsdell, J. S. (2007). Optimization of ciguatoxin extraction method from blood for Pacific ciguatoxin (P-CTX-1). Toxicon : Official Journal of the International Society On Toxinology, 49(1), 100-5.
Bottein Dechraoui MY, Wang Z, Ramsdell JS. Optimization of Ciguatoxin Extraction Method From Blood for Pacific Ciguatoxin (P-CTX-1). Toxicon. 2007;49(1):100-5. PubMed PMID: 17113119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of ciguatoxin extraction method from blood for Pacific ciguatoxin (P-CTX-1). AU - Bottein Dechraoui,Marie-Yasmine, AU - Wang,Zhihong, AU - Ramsdell,John S, Y1 - 2006/10/14/ PY - 2006/05/23/received PY - 2006/09/26/revised PY - 2006/10/09/accepted PY - 2006/11/23/pubmed PY - 2007/3/3/medline PY - 2006/11/23/entrez SP - 100 EP - 5 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 49 IS - 1 N2 - Ciguatera diagnosis relies on clinical observations associated with a recent consumption of fish. Although needed, direct confirmation of exposure in subjects showing ciguatera disease symptoms is currently unavailable. We previously reported that ciguatoxins were measurable in the blood of mice exposed to extracts of Pacific ciguatoxins isolated from Gambierdiscus polynesiensis, and of Indian Ocean or Caribbean Sea ciguatoxins, isolated from fish. Although highly efficient for extracting spiked purified Caribbean-CTX-1, the methanolic extraction method previously described is found here to yield only 6% recovery of spiked Pacific-CTX-1 (P-CTX-1). We report in this short communication a substantially modified method for ciguatoxin extraction from both dried and fresh blood. With this method, toxin measurement is directly accomplished in acetonitrile deproteinated whole fresh blood or phosphate buffer solution (PBS) eluted dried blood using the N2A cell-based assay. Spike studies using increasing concentrations of purified ciguatoxins reveal linear (r2 above 0.87 for all toxins) and overall efficient toxin recoveries (62%, 96%, and 96% from fresh blood and 75%, 90%, and 74% from dried blood, for C-CTX-1, P-CTX-3C, and P-CTX-1, respectively). Comparative blood matrix analysis for P-CTX-1 recovery shows increased recovery of ciguatoxin activity from whole fresh blood than from dried blood, greater by 20% in P-CTX-1 spiked mice blood and by over 85% in P-CTX-1 exposed mouse blood. In conclusion, both Caribbean and Pacific ciguatoxins can be readily extracted from blood using this modified method; however, in the case of P-CTX-1 we find that fresh blood is optimal. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/17113119/Optimization_of_ciguatoxin_extraction_method_from_blood_for_Pacific_ciguatoxin__P_CTX_1__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(06)00377-1 DB - PRIME DP - Unbound Medicine ER -