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Effects of parathyroid hormone on the accumulation of cyclic AMP in bone of vitamin D-deficient rats.
Endocrinology. 1975 Nov; 97(5):1288-93.E

Abstract

The mechanism of skeletal refractoriness to parathyroid hormone (PTH) in vitamine D-deficient animals was studied in terms of the adenylate cyclase-cyclic AMP system in rat calvaria. In vitamin D-deficient, thyroparathyroidectomized rats, plasma calcium concentration was not elevated by iv administration of PTH, while responsiveness to the hormone was recovered within 24 h after a single dose (2.5 mug) of vitamin D3. In spite of the remarkable dependency of PTH on vitamin D for mobilization of calcium from bone, PTH stimulated adenylate cyclase activity in particulate bone cell fractions in vitro. PTH also enhanced the levels of cyclic AMP in the skeletal tissues of vitamin D-deficient rats in vivo and in vitro to an extent similar to those found in rats given 2.5 mug of D3. Administration of theophylline or dibutyryl cyclic AMP to the vitamin D-deficient rats did not cause any significant hypercalcemic effects, while these drugs enhanced plasma calcium concentration significantly in the rats given vitamin D3. These data strongly indicate that the cause of the skeletal refractoriness to PTH in vitamin D-deficient animals is not a defective activation of adenylate cyclase, but must be related to a later step or steps in the biochemical events leading to bone cell activation.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

171152

Citation

Kakuta, S, et al. "Effects of Parathyroid Hormone On the Accumulation of Cyclic AMP in Bone of Vitamin D-deficient Rats." Endocrinology, vol. 97, no. 5, 1975, pp. 1288-93.
Kakuta S, Suda T, Sasaki S, et al. Effects of parathyroid hormone on the accumulation of cyclic AMP in bone of vitamin D-deficient rats. Endocrinology. 1975;97(5):1288-93.
Kakuta, S., Suda, T., Sasaki, S., Kimura, N., & Nagata, N. (1975). Effects of parathyroid hormone on the accumulation of cyclic AMP in bone of vitamin D-deficient rats. Endocrinology, 97(5), 1288-93.
Kakuta S, et al. Effects of Parathyroid Hormone On the Accumulation of Cyclic AMP in Bone of Vitamin D-deficient Rats. Endocrinology. 1975;97(5):1288-93. PubMed PMID: 171152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of parathyroid hormone on the accumulation of cyclic AMP in bone of vitamin D-deficient rats. AU - Kakuta,S, AU - Suda,T, AU - Sasaki,S, AU - Kimura,N, AU - Nagata,N, PY - 1975/11/1/pubmed PY - 1975/11/1/medline PY - 1975/11/1/entrez SP - 1288 EP - 93 JF - Endocrinology JO - Endocrinology VL - 97 IS - 5 N2 - The mechanism of skeletal refractoriness to parathyroid hormone (PTH) in vitamine D-deficient animals was studied in terms of the adenylate cyclase-cyclic AMP system in rat calvaria. In vitamin D-deficient, thyroparathyroidectomized rats, plasma calcium concentration was not elevated by iv administration of PTH, while responsiveness to the hormone was recovered within 24 h after a single dose (2.5 mug) of vitamin D3. In spite of the remarkable dependency of PTH on vitamin D for mobilization of calcium from bone, PTH stimulated adenylate cyclase activity in particulate bone cell fractions in vitro. PTH also enhanced the levels of cyclic AMP in the skeletal tissues of vitamin D-deficient rats in vivo and in vitro to an extent similar to those found in rats given 2.5 mug of D3. Administration of theophylline or dibutyryl cyclic AMP to the vitamin D-deficient rats did not cause any significant hypercalcemic effects, while these drugs enhanced plasma calcium concentration significantly in the rats given vitamin D3. These data strongly indicate that the cause of the skeletal refractoriness to PTH in vitamin D-deficient animals is not a defective activation of adenylate cyclase, but must be related to a later step or steps in the biochemical events leading to bone cell activation. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/171152/Effects_of_parathyroid_hormone_on_the_accumulation_of_cyclic_AMP_in_bone_of_vitamin_D_deficient_rats_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/endo-97-5-1288 DB - PRIME DP - Unbound Medicine ER -