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Elimination of false-positive Histoplasma antigenemia caused by human anti-rabbit antibodies in the second-generation Histoplasma antigen assay.
Transpl Infect Dis. 2006 Dec; 8(4):219-21.TI

Abstract

False-positive Histoplasma antigenemia was reported in solid organ allograft recipients who had received rabbit anti-thymocyte globulin (RATG, RATG) caused by human anti-rabbit antibodies (HARA). A second-generation Histoplasma antigen detection assay was developed to overcome false positivity caused by HARA. With the second-generation assay, false-positive results were eliminated in 18 of 19 cases without reduction in the sensitivity in patients with histoplasmosis. In fact, sensitivity for detection of antigenuria in patients with acquired immunodeficiency syndrome and disseminated histoplasmosis was higher in the second-generation assay. Physicians should be aware of the potential for false-positive results in sandwich immunoassays in specimens from patients who have received RATG.

Authors+Show Affiliations

MiraVista Diagnostics, Indianapolis, Indiana 46241, USA. jwheat@miravistalabs.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17116135

Citation

Wheat, L J., et al. "Elimination of False-positive Histoplasma Antigenemia Caused By Human Anti-rabbit Antibodies in the Second-generation Histoplasma Antigen Assay." Transplant Infectious Disease : an Official Journal of the Transplantation Society, vol. 8, no. 4, 2006, pp. 219-21.
Wheat LJ, Connolly P, Durkin M, et al. Elimination of false-positive Histoplasma antigenemia caused by human anti-rabbit antibodies in the second-generation Histoplasma antigen assay. Transpl Infect Dis. 2006;8(4):219-21.
Wheat, L. J., Connolly, P., Durkin, M., Book, B. K., & Pescovitz, M. D. (2006). Elimination of false-positive Histoplasma antigenemia caused by human anti-rabbit antibodies in the second-generation Histoplasma antigen assay. Transplant Infectious Disease : an Official Journal of the Transplantation Society, 8(4), 219-21.
Wheat LJ, et al. Elimination of False-positive Histoplasma Antigenemia Caused By Human Anti-rabbit Antibodies in the Second-generation Histoplasma Antigen Assay. Transpl Infect Dis. 2006;8(4):219-21. PubMed PMID: 17116135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elimination of false-positive Histoplasma antigenemia caused by human anti-rabbit antibodies in the second-generation Histoplasma antigen assay. AU - Wheat,L J, AU - Connolly,P, AU - Durkin,M, AU - Book,B K, AU - Pescovitz,M D, PY - 2006/11/23/pubmed PY - 2007/3/9/medline PY - 2006/11/23/entrez SP - 219 EP - 21 JF - Transplant infectious disease : an official journal of the Transplantation Society JO - Transpl Infect Dis VL - 8 IS - 4 N2 - False-positive Histoplasma antigenemia was reported in solid organ allograft recipients who had received rabbit anti-thymocyte globulin (RATG, RATG) caused by human anti-rabbit antibodies (HARA). A second-generation Histoplasma antigen detection assay was developed to overcome false positivity caused by HARA. With the second-generation assay, false-positive results were eliminated in 18 of 19 cases without reduction in the sensitivity in patients with histoplasmosis. In fact, sensitivity for detection of antigenuria in patients with acquired immunodeficiency syndrome and disseminated histoplasmosis was higher in the second-generation assay. Physicians should be aware of the potential for false-positive results in sandwich immunoassays in specimens from patients who have received RATG. SN - 1398-2273 UR - https://www.unboundmedicine.com/medline/citation/17116135/Elimination_of_false_positive_Histoplasma_antigenemia_caused_by_human_anti_rabbit_antibodies_in_the_second_generation_Histoplasma_antigen_assay_ L2 - https://doi.org/10.1111/j.1399-3062.2006.00151.x DB - PRIME DP - Unbound Medicine ER -