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A new ATP-sensitive potassium channel opener protects endothelial function in cultured aortic endothelial cells.
Cardiovasc Res. 2007 Feb 01; 73(3):497-503.CR

Abstract

OBJECTIVE

Endothelial dysfunction is an early risk factor for cardiovascular disease and hypertension. Mechanisms that participate in endothelial dysfunction include reduced nitric oxide (NO) generation and increased endothelin-1 (ET-1) generation. Endothelial ATP-sensitive potassium (K(ATP)) channels are responsible for maintaining the resting potential of endothelial cells and modulating the release of vasoactive compounds. We hypothesized that activation of endothelial K(ATP) channels might result in the protection against endothelial dysfunction.

METHODS

Using cultured bovine or rat aortic endothelial cells, we examined the effects of a new K(ATP) channels opener, iptakalim, on the secretion of vasoactive substances. We also investigated its effects on the expression of adhesion molecules in metabolically disturbed cultured endothelial cells.

RESULTS

In cultured aortic endothelial cells, iptakalim caused a concentration-dependent inhibition of ET-1 release and synthesis that correlated with reduced levels of mRNA for ET-1 and endothelin-converting enzyme. These effects of iptakalim were significantly inhibited by pretreatment with glibenclamide (a K(ATP) channel blocker) for 1 h. Similarly, iptakalim enhanced the release of NO in a concentration-dependent manner and increased basal levels of free intracellular calcium. Iptakalim at the concentrations of 100 and 1000 microM increased the activities of NO synthase (NOS) significantly. After the activity of NOS was blocked by L-N(omega)-nitro-arginine methyl ester (L-NAME), the inhibition of iptakalim on ET-1 release was abolished. In endothelial cell models of metabolic disturbance induced by low-density lipoprotein, homocysteine, or hyperglycemia, treatment with iptakalim could inhibit the overexpression of monocyte chemoattractant protein-1 (MCP-1), Intercellular adhesive molecule-1 (ICAM-1), and vascular cell adhesive molecule-1 (VCAM-1) mRNA.

CONCLUSION

Iptakalim is a promising drug that could protect against endothelial dysfunction through activating K(ATP) channels in endothelial cells.

Authors+Show Affiliations

Department of Cardiovascular Pharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. wh9588@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17116295

Citation

Wang, Hai, et al. "A New ATP-sensitive Potassium Channel Opener Protects Endothelial Function in Cultured Aortic Endothelial Cells." Cardiovascular Research, vol. 73, no. 3, 2007, pp. 497-503.
Wang H, Long C, Duan Z, et al. A new ATP-sensitive potassium channel opener protects endothelial function in cultured aortic endothelial cells. Cardiovasc Res. 2007;73(3):497-503.
Wang, H., Long, C., Duan, Z., Shi, C., Jia, G., & Zhang, Y. (2007). A new ATP-sensitive potassium channel opener protects endothelial function in cultured aortic endothelial cells. Cardiovascular Research, 73(3), 497-503.
Wang H, et al. A New ATP-sensitive Potassium Channel Opener Protects Endothelial Function in Cultured Aortic Endothelial Cells. Cardiovasc Res. 2007 Feb 1;73(3):497-503. PubMed PMID: 17116295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new ATP-sensitive potassium channel opener protects endothelial function in cultured aortic endothelial cells. AU - Wang,Hai, AU - Long,Chaoliang, AU - Duan,Zhibian, AU - Shi,Cuige, AU - Jia,Guodong, AU - Zhang,Yingli, Y1 - 2006/10/14/ PY - 2006/04/11/received PY - 2006/10/07/revised PY - 2006/10/09/accepted PY - 2006/11/23/pubmed PY - 2007/6/15/medline PY - 2006/11/23/entrez SP - 497 EP - 503 JF - Cardiovascular research JO - Cardiovasc Res VL - 73 IS - 3 N2 - OBJECTIVE: Endothelial dysfunction is an early risk factor for cardiovascular disease and hypertension. Mechanisms that participate in endothelial dysfunction include reduced nitric oxide (NO) generation and increased endothelin-1 (ET-1) generation. Endothelial ATP-sensitive potassium (K(ATP)) channels are responsible for maintaining the resting potential of endothelial cells and modulating the release of vasoactive compounds. We hypothesized that activation of endothelial K(ATP) channels might result in the protection against endothelial dysfunction. METHODS: Using cultured bovine or rat aortic endothelial cells, we examined the effects of a new K(ATP) channels opener, iptakalim, on the secretion of vasoactive substances. We also investigated its effects on the expression of adhesion molecules in metabolically disturbed cultured endothelial cells. RESULTS: In cultured aortic endothelial cells, iptakalim caused a concentration-dependent inhibition of ET-1 release and synthesis that correlated with reduced levels of mRNA for ET-1 and endothelin-converting enzyme. These effects of iptakalim were significantly inhibited by pretreatment with glibenclamide (a K(ATP) channel blocker) for 1 h. Similarly, iptakalim enhanced the release of NO in a concentration-dependent manner and increased basal levels of free intracellular calcium. Iptakalim at the concentrations of 100 and 1000 microM increased the activities of NO synthase (NOS) significantly. After the activity of NOS was blocked by L-N(omega)-nitro-arginine methyl ester (L-NAME), the inhibition of iptakalim on ET-1 release was abolished. In endothelial cell models of metabolic disturbance induced by low-density lipoprotein, homocysteine, or hyperglycemia, treatment with iptakalim could inhibit the overexpression of monocyte chemoattractant protein-1 (MCP-1), Intercellular adhesive molecule-1 (ICAM-1), and vascular cell adhesive molecule-1 (VCAM-1) mRNA. CONCLUSION: Iptakalim is a promising drug that could protect against endothelial dysfunction through activating K(ATP) channels in endothelial cells. SN - 0008-6363 UR - https://www.unboundmedicine.com/medline/citation/17116295/A_new_ATP_sensitive_potassium_channel_opener_protects_endothelial_function_in_cultured_aortic_endothelial_cells_ DB - PRIME DP - Unbound Medicine ER -