Tags

Type your tag names separated by a space and hit enter

Utility of the Determine Syphilis TP rapid test in commercial sex venues in Peru.
Sex Transm Infect. 2006 Dec; 82 Suppl 5:v22-5.ST

Abstract

OBJECTIVES

This study sought to evaluate the utility of the Determine Syphilis TP test performed in Peruvian commercial sex venues for the detection of active syphilis; and determine the feasibility of integrating rapid syphilis testing for female sex workers (FSW) into existing health outreach services.

METHODS

We tested 3586 female sex workers for syphilis by Determine in the field using whole blood fingerstick, and by rapid plasma reagin (RPR) and Treponema pallidum haemagglutination assay (TPHA) in a central laboratory in Lima using sera.

RESULTS

97.4% of the FSW offered rapid syphilis testing participated; and among those who tested positive, 87% visited the local health centre for treatment. More than twice as many specimens were RPR reactive using serum in Lima (5.7%) than tested positive by whole blood Determine in the field (2.8%), and although most were confirmed by TPHA, only a small proportion (0.7%) were RPR reactive at >or=1:8 dilutions, and likely indicating active syphilis. Sensitivity, specificity and positive predictive value of the Determine Syphilis TP test in whole blood when compared to serum RPR reactivity at any dilution confirmed by TPHA as the gold standard were 39.3%, 99.2% and 71.4%, respectively. Sensitivity improved to 64.0% when using serum RPR >or=1:8 confirmed by TPHA. Invalid tests were rare (0.3%).

CONCLUSIONS

Rapid syphilis testing in sex work venues proved feasible, but Determine using whole blood obtained by fingerstick was substantially less sensitive than reported in previous laboratory-based studies using serum. Although easy to perform in outreach venues, the utility of this rapid syphilis test was relatively low in settings where a large proportion of the targeted population has been previously tested and treated.

Authors+Show Affiliations

Unidad de Epidemiología, Enfermedades de Transmisión Sexual y SIDA, Facultad de Salud Pública y Administración, Universidad Peruana Cayetano Heredia, Lima, Peru.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17116642

Citation

Campos, P E., et al. "Utility of the Determine Syphilis TP Rapid Test in Commercial Sex Venues in Peru." Sexually Transmitted Infections, vol. 82 Suppl 5, 2006, pp. v22-5.
Campos PE, Buffardi AL, Chiappe M, et al. Utility of the Determine Syphilis TP rapid test in commercial sex venues in Peru. Sex Transm Infect. 2006;82 Suppl 5:v22-5.
Campos, P. E., Buffardi, A. L., Chiappe, M., Buendía, C., Garcia, P. J., Carcamo, C. P., Garnett, G., White, P., & Holmes, K. K. (2006). Utility of the Determine Syphilis TP rapid test in commercial sex venues in Peru. Sexually Transmitted Infections, 82 Suppl 5, v22-5.
Campos PE, et al. Utility of the Determine Syphilis TP Rapid Test in Commercial Sex Venues in Peru. Sex Transm Infect. 2006;82 Suppl 5:v22-5. PubMed PMID: 17116642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Utility of the Determine Syphilis TP rapid test in commercial sex venues in Peru. AU - Campos,P E, AU - Buffardi,A L, AU - Chiappe,M, AU - Buendía,C, AU - Garcia,P J, AU - Carcamo,C P, AU - Garnett,G, AU - White,P, AU - Holmes,K K, Y1 - 2006/11/20/ PY - 2006/11/23/pubmed PY - 2007/3/21/medline PY - 2006/11/23/entrez SP - v22 EP - 5 JF - Sexually transmitted infections JO - Sex Transm Infect VL - 82 Suppl 5 N2 - OBJECTIVES: This study sought to evaluate the utility of the Determine Syphilis TP test performed in Peruvian commercial sex venues for the detection of active syphilis; and determine the feasibility of integrating rapid syphilis testing for female sex workers (FSW) into existing health outreach services. METHODS: We tested 3586 female sex workers for syphilis by Determine in the field using whole blood fingerstick, and by rapid plasma reagin (RPR) and Treponema pallidum haemagglutination assay (TPHA) in a central laboratory in Lima using sera. RESULTS: 97.4% of the FSW offered rapid syphilis testing participated; and among those who tested positive, 87% visited the local health centre for treatment. More than twice as many specimens were RPR reactive using serum in Lima (5.7%) than tested positive by whole blood Determine in the field (2.8%), and although most were confirmed by TPHA, only a small proportion (0.7%) were RPR reactive at >or=1:8 dilutions, and likely indicating active syphilis. Sensitivity, specificity and positive predictive value of the Determine Syphilis TP test in whole blood when compared to serum RPR reactivity at any dilution confirmed by TPHA as the gold standard were 39.3%, 99.2% and 71.4%, respectively. Sensitivity improved to 64.0% when using serum RPR >or=1:8 confirmed by TPHA. Invalid tests were rare (0.3%). CONCLUSIONS: Rapid syphilis testing in sex work venues proved feasible, but Determine using whole blood obtained by fingerstick was substantially less sensitive than reported in previous laboratory-based studies using serum. Although easy to perform in outreach venues, the utility of this rapid syphilis test was relatively low in settings where a large proportion of the targeted population has been previously tested and treated. SN - 1368-4973 UR - https://www.unboundmedicine.com/medline/citation/17116642/Utility_of_the_Determine_Syphilis_TP_rapid_test_in_commercial_sex_venues_in_Peru_ L2 - https://sti.bmj.com/lookup/pmidlookup?view=long&pmid=17116642 DB - PRIME DP - Unbound Medicine ER -