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A prospective study comparing quantitative Cytomegalovirus (CMV) polymerase chain reaction in plasma and pp65 antigenemia assay in monitoring patients after allogeneic stem cell transplantation.
BMC Infect Dis. 2006 Nov 21; 6:167.BI

Abstract

BACKGROUND

Low levels of Cytomegalovirus (CMV) viral load are frequently detected following allogeneic stem cell transplantation (SCT) and CMV disease may still develop in some allogeneic SCT patients who have negative pp65-antigenemia (pp65-Ag) or undetectable DNA. Pp65Ag is a sensitive method to diagnose CMV infection. Quantitative CMV-DNA PCR assay in plasma has been proposed to monitor CMV infection in SCT patients. We evaluated the clinical utility of pp65Ag and PCR assay in plasma of SCT recipients.

METHODS

In a prospective longitudinal study, 38 consecutive patients at risk of CMV infection (donor and/or recipient CMV seropositive) were weekly monitored for CMV infection by both quantitative CMV-PCR in plasma (COBAS AMPLICOR CMV MONITOR) and pp65 Ag, during the first 100 days after SCT.

RESULTS

A total of 534 blood samples were simultaneously analysed for pp65Ag and PCR. Overall, 28/38 patients (74%) had active CMV infection within 100 days from SCT. In 16 patients, CMV was first detected by pp65 Ag alone; in 5 patients by both methods and in 6 by PCR assay alone; one patient had CMV biopsy-proven intestinal disease without pp65Ag and PCR assays positivity before CMV disease. Overall, three patients developed intestinal CMV disease (7.9%): one had negative both pp65Ag and PCR assays before CMV disease, one had disease and concomitant positivity of both methods, while in the remaining patient, only pp65Ag was positive before CMV disease.

CONCLUSION

Plasma PCR(COBAS AMPLICOR CMV MONITOR) and pp65Ag assays were effective in detecting CMV infection, however, discordance between both methods were frequently observed. Plasma PCR and pp65Ag assays may be complementary for diagnosis and management of CMV infection.

Authors+Show Affiliations

Department of Cellular Biotechnology and Hematology, Univ, La Sapienza, Rome, Italy. gentile@bce.uniroma1.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

17118205

Citation

Gentile, Giuseppe, et al. "A Prospective Study Comparing Quantitative Cytomegalovirus (CMV) Polymerase Chain Reaction in Plasma and Pp65 Antigenemia Assay in Monitoring Patients After Allogeneic Stem Cell Transplantation." BMC Infectious Diseases, vol. 6, 2006, p. 167.
Gentile G, Picardi A, Capobianchi A, et al. A prospective study comparing quantitative Cytomegalovirus (CMV) polymerase chain reaction in plasma and pp65 antigenemia assay in monitoring patients after allogeneic stem cell transplantation. BMC Infect Dis. 2006;6:167.
Gentile, G., Picardi, A., Capobianchi, A., Spagnoli, A., Cudillo, L., Dentamaro, T., Tendas, A., Cupelli, L., Ciotti, M., Volpi, A., Amadori, S., Martino, P., & de Fabritiis, P. (2006). A prospective study comparing quantitative Cytomegalovirus (CMV) polymerase chain reaction in plasma and pp65 antigenemia assay in monitoring patients after allogeneic stem cell transplantation. BMC Infectious Diseases, 6, 167.
Gentile G, et al. A Prospective Study Comparing Quantitative Cytomegalovirus (CMV) Polymerase Chain Reaction in Plasma and Pp65 Antigenemia Assay in Monitoring Patients After Allogeneic Stem Cell Transplantation. BMC Infect Dis. 2006 Nov 21;6:167. PubMed PMID: 17118205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A prospective study comparing quantitative Cytomegalovirus (CMV) polymerase chain reaction in plasma and pp65 antigenemia assay in monitoring patients after allogeneic stem cell transplantation. AU - Gentile,Giuseppe, AU - Picardi,Alessandra, AU - Capobianchi,Angela, AU - Spagnoli,Alessandra, AU - Cudillo,Laura, AU - Dentamaro,Teresa, AU - Tendas,Andrea, AU - Cupelli,Luca, AU - Ciotti,Marco, AU - Volpi,Antonio, AU - Amadori,Sergio, AU - Martino,Pietro, AU - de Fabritiis,Paolo, Y1 - 2006/11/21/ PY - 2006/05/23/received PY - 2006/11/21/accepted PY - 2006/11/23/pubmed PY - 2006/12/22/medline PY - 2006/11/23/entrez SP - 167 EP - 167 JF - BMC infectious diseases JO - BMC Infect Dis VL - 6 N2 - BACKGROUND: Low levels of Cytomegalovirus (CMV) viral load are frequently detected following allogeneic stem cell transplantation (SCT) and CMV disease may still develop in some allogeneic SCT patients who have negative pp65-antigenemia (pp65-Ag) or undetectable DNA. Pp65Ag is a sensitive method to diagnose CMV infection. Quantitative CMV-DNA PCR assay in plasma has been proposed to monitor CMV infection in SCT patients. We evaluated the clinical utility of pp65Ag and PCR assay in plasma of SCT recipients. METHODS: In a prospective longitudinal study, 38 consecutive patients at risk of CMV infection (donor and/or recipient CMV seropositive) were weekly monitored for CMV infection by both quantitative CMV-PCR in plasma (COBAS AMPLICOR CMV MONITOR) and pp65 Ag, during the first 100 days after SCT. RESULTS: A total of 534 blood samples were simultaneously analysed for pp65Ag and PCR. Overall, 28/38 patients (74%) had active CMV infection within 100 days from SCT. In 16 patients, CMV was first detected by pp65 Ag alone; in 5 patients by both methods and in 6 by PCR assay alone; one patient had CMV biopsy-proven intestinal disease without pp65Ag and PCR assays positivity before CMV disease. Overall, three patients developed intestinal CMV disease (7.9%): one had negative both pp65Ag and PCR assays before CMV disease, one had disease and concomitant positivity of both methods, while in the remaining patient, only pp65Ag was positive before CMV disease. CONCLUSION: Plasma PCR(COBAS AMPLICOR CMV MONITOR) and pp65Ag assays were effective in detecting CMV infection, however, discordance between both methods were frequently observed. Plasma PCR and pp65Ag assays may be complementary for diagnosis and management of CMV infection. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/17118205/A_prospective_study_comparing_quantitative_Cytomegalovirus__CMV__polymerase_chain_reaction_in_plasma_and_pp65_antigenemia_assay_in_monitoring_patients_after_allogeneic_stem_cell_transplantation_ L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-6-167 DB - PRIME DP - Unbound Medicine ER -