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Electrochemical detection of 17beta-estradiol using DNA aptamer immobilized gold electrode chip.
Biosens Bioelectron. 2007 May 15; 22(11):2525-31.BB

Abstract

An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17beta-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 x 10(14) DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17beta-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17beta-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.

Authors+Show Affiliations

Kim YS
College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
Jung HS
No affiliation info available
Matsuura T
No affiliation info available
Lee HY
No affiliation info available
Kawai T
No affiliation info available
Gu MB
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesElectrochemistryElectrodesEquipment DesignEquipment Failure AnalysisEstradiolGoldMicroelectrodesOligonucleotide Array Sequence AnalysisReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17118645

Citation

Kim, Yeon Seok, et al. "Electrochemical Detection of 17beta-estradiol Using DNA Aptamer Immobilized Gold Electrode Chip." Biosensors & Bioelectronics, vol. 22, no. 11, 2007, pp. 2525-31.
Kim YS, Jung HS, Matsuura T, et al. Electrochemical detection of 17beta-estradiol using DNA aptamer immobilized gold electrode chip. Biosens Bioelectron. 2007;22(11):2525-31.
Kim, Y. S., Jung, H. S., Matsuura, T., Lee, H. Y., Kawai, T., & Gu, M. B. (2007). Electrochemical detection of 17beta-estradiol using DNA aptamer immobilized gold electrode chip. Biosensors & Bioelectronics, 22(11), 2525-31.
Kim YS, et al. Electrochemical Detection of 17beta-estradiol Using DNA Aptamer Immobilized Gold Electrode Chip. Biosens Bioelectron. 2007 May 15;22(11):2525-31. PubMed PMID: 17118645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical detection of 17beta-estradiol using DNA aptamer immobilized gold electrode chip. AU - Kim,Yeon Seok, AU - Jung,Ho Sup, AU - Matsuura,Toshihiko, AU - Lee,Hea Yeon, AU - Kawai,Tomoji, AU - Gu,Man Bock, Y1 - 2006/11/21/ PY - 2006/06/05/received PY - 2006/09/27/revised PY - 2006/10/03/accepted PY - 2006/11/23/pubmed PY - 2007/6/28/medline PY - 2006/11/23/entrez SP - 2525 EP - 31 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 11 N2 - An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17beta-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 x 10(14) DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17beta-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17beta-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17118645/Electrochemical_detection_of_17beta_estradiol_using_DNA_aptamer_immobilized_gold_electrode_chip_ DB - PRIME DP - Unbound Medicine ER -