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Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid-phase extraction procedure.
J Sep Sci. 2006 Oct; 29(15):2322-9.JS

Abstract

A precise and feasible HPLC method has been developed for the analysis of amphetamine (AMPH), methamphetamine (MAMPH) and methylenedioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 mm x 4.6 mm, 5 microm) column maintained at 30 degrees C lasts about 17 min, using a mobile phase composed of ACN (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100-2000 ng/mL concentration range for AMPH and MAMPH and in the 12-2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values greater than 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of AMPH users.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum, University of Bologna, Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17120816

Citation

Bugamelli, Francesca, et al. "Determination of Amphetamines in Human Urine By Liquid Chromatography With Fluorimetric Detection Using a Solid-phase Extraction Procedure." Journal of Separation Science, vol. 29, no. 15, 2006, pp. 2322-9.
Bugamelli F, Mandrioli R, Cavallini A, et al. Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid-phase extraction procedure. J Sep Sci. 2006;29(15):2322-9.
Bugamelli, F., Mandrioli, R., Cavallini, A., Baccini, C., Conti, M., & Raggi, M. A. (2006). Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid-phase extraction procedure. Journal of Separation Science, 29(15), 2322-9.
Bugamelli F, et al. Determination of Amphetamines in Human Urine By Liquid Chromatography With Fluorimetric Detection Using a Solid-phase Extraction Procedure. J Sep Sci. 2006;29(15):2322-9. PubMed PMID: 17120816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid-phase extraction procedure. AU - Bugamelli,Francesca, AU - Mandrioli,Roberto, AU - Cavallini,Annalisa, AU - Baccini,Cesare, AU - Conti,Matteo, AU - Raggi,Maria Augusta, PY - 2006/11/24/pubmed PY - 2007/8/31/medline PY - 2006/11/24/entrez SP - 2322 EP - 9 JF - Journal of separation science JO - J Sep Sci VL - 29 IS - 15 N2 - A precise and feasible HPLC method has been developed for the analysis of amphetamine (AMPH), methamphetamine (MAMPH) and methylenedioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 mm x 4.6 mm, 5 microm) column maintained at 30 degrees C lasts about 17 min, using a mobile phase composed of ACN (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100-2000 ng/mL concentration range for AMPH and MAMPH and in the 12-2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values greater than 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of AMPH users. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/17120816/Determination_of_amphetamines_in_human_urine_by_liquid_chromatography_with_fluorimetric_detection_using_a_solid_phase_extraction_procedure_ L2 - https://doi.org/10.1002/jssc.200600139 DB - PRIME DP - Unbound Medicine ER -