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Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with Bax and Bak induction.
Clin Cancer Res. 2006 Nov 15; 12(22):6836-43.CC

Abstract

PURPOSE

The present study was undertaken to determine the effect of garlic constituent diallyl trisulfide (DATS) on growth of PC-3 human prostate cancer xenograft in vivo.

EXPERIMENTAL DESIGN

DATS was given orally (6 micromoL, thrice weekly) to male athymic mice s.c. implanted with PC-3 cells. Tumor sections from control and DATS-treated mice were examined for apoptotic bodies by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Protein levels of apoptosis and cell cycle regulating proteins in tumor tissues of control and DATS-treated mice were determined by immunoblotting. The effect of DATS treatment on in vivo angiogenesis was determined by immunohistochemical analysis of CD31 in tumors.

RESULTS

Oral gavage of DATS significantly retarded growth of PC-3 xenografts in athymic mice without causing weight loss. For instance, 20 days after starting therapy, the average tumor volume in control mice was approximately 3-fold higher compared with DATS-treated mice. Tumors from DATS-treated mice exhibited a markedly higher count of apoptotic bodies compared with control tumors. Consistent with the results in cultured PC-3 cells, the DATS-mediated suppression of PC-3 xenograft growth correlated with induction of proapoptotic proteins Bax and Bak. Although DATS treatment inhibited migration of cultured PC-3 cells in association with down-regulation of vascular endothelial growth factor receptor-2 protein, formation of new blood vessels was comparable in tumors of control and DATS-treated mice as judged by CD31 immunostaining.

CONCLUSIONS

The present study indicates that DATS administration inhibits growth of PC-3 xenografts in vivo in association with induction of Bax and Bak.

Authors+Show Affiliations

Department of Pharmacology and University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17121905

Citation

Xiao, Dong, et al. "Diallyl Trisulfide Suppresses Growth of PC-3 Human Prostate Cancer Xenograft in Vivo in Association With Bax and Bak Induction." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 12, no. 22, 2006, pp. 6836-43.
Xiao D, Lew KL, Kim YA, et al. Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with Bax and Bak induction. Clin Cancer Res. 2006;12(22):6836-43.
Xiao, D., Lew, K. L., Kim, Y. A., Zeng, Y., Hahm, E. R., Dhir, R., & Singh, S. V. (2006). Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with Bax and Bak induction. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 12(22), 6836-43.
Xiao D, et al. Diallyl Trisulfide Suppresses Growth of PC-3 Human Prostate Cancer Xenograft in Vivo in Association With Bax and Bak Induction. Clin Cancer Res. 2006 Nov 15;12(22):6836-43. PubMed PMID: 17121905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with Bax and Bak induction. AU - Xiao,Dong, AU - Lew,Karen L, AU - Kim,Young-Ae, AU - Zeng,Yan, AU - Hahm,Eun-Ryeong, AU - Dhir,Rajiv, AU - Singh,Shivendra V, PY - 2006/11/24/pubmed PY - 2007/2/7/medline PY - 2006/11/24/entrez SP - 6836 EP - 43 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin. Cancer Res. VL - 12 IS - 22 N2 - PURPOSE: The present study was undertaken to determine the effect of garlic constituent diallyl trisulfide (DATS) on growth of PC-3 human prostate cancer xenograft in vivo. EXPERIMENTAL DESIGN: DATS was given orally (6 micromoL, thrice weekly) to male athymic mice s.c. implanted with PC-3 cells. Tumor sections from control and DATS-treated mice were examined for apoptotic bodies by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Protein levels of apoptosis and cell cycle regulating proteins in tumor tissues of control and DATS-treated mice were determined by immunoblotting. The effect of DATS treatment on in vivo angiogenesis was determined by immunohistochemical analysis of CD31 in tumors. RESULTS: Oral gavage of DATS significantly retarded growth of PC-3 xenografts in athymic mice without causing weight loss. For instance, 20 days after starting therapy, the average tumor volume in control mice was approximately 3-fold higher compared with DATS-treated mice. Tumors from DATS-treated mice exhibited a markedly higher count of apoptotic bodies compared with control tumors. Consistent with the results in cultured PC-3 cells, the DATS-mediated suppression of PC-3 xenograft growth correlated with induction of proapoptotic proteins Bax and Bak. Although DATS treatment inhibited migration of cultured PC-3 cells in association with down-regulation of vascular endothelial growth factor receptor-2 protein, formation of new blood vessels was comparable in tumors of control and DATS-treated mice as judged by CD31 immunostaining. CONCLUSIONS: The present study indicates that DATS administration inhibits growth of PC-3 xenografts in vivo in association with induction of Bax and Bak. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/17121905/Diallyl_trisulfide_suppresses_growth_of_PC_3_human_prostate_cancer_xenograft_in_vivo_in_association_with_Bax_and_Bak_induction_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=17121905 DB - PRIME DP - Unbound Medicine ER -