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The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo.
Cell 1991; 65(7):1233-42Cell

Abstract

We show that the rate of degradation of RNAI, an anti-sense repressor of the replication primer RNAII, is a key element of control in the replication of ColE1-type plasmids in vivo. Cleavage of RNAI by RNAase E, a ribosomal RNA-processing enzyme encoded or controlled by the rne (also known as ams) locus, relieves repression by endonucleolytically converting RNAI to a very rapidly decaying product, pRNAI-5. A 5' triphosphate-terminated homolog of pRNAI-5 is degraded slowly and consequently inhibits replication. Nucleotide substitutions within the RNAase E cleavage sequence alter RNAI half-life and plasmid copy number, changing also the incompatibility phenotype. RNAI variants lacking the sequence cleaved by RNAase E are eliminated by growth rate-dependent degradation, resulting in growth-responsive control of plasmid replication and copy number.

Authors+Show Affiliations

Institute of Molecular Biology, Academia Sinica, Nankang Taipei, Taiwan, Republic of China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1712252

Citation

Lin-Chao, S, and S N. Cohen. "The Rate of Processing and Degradation of Antisense RNAI Regulates the Replication of ColE1-type Plasmids in Vivo." Cell, vol. 65, no. 7, 1991, pp. 1233-42.
Lin-Chao S, Cohen SN. The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. Cell. 1991;65(7):1233-42.
Lin-Chao, S., & Cohen, S. N. (1991). The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. Cell, 65(7), pp. 1233-42.
Lin-Chao S, Cohen SN. The Rate of Processing and Degradation of Antisense RNAI Regulates the Replication of ColE1-type Plasmids in Vivo. Cell. 1991 Jun 28;65(7):1233-42. PubMed PMID: 1712252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. AU - Lin-Chao,S, AU - Cohen,S N, PY - 1991/6/28/pubmed PY - 1991/6/28/medline PY - 1991/6/28/entrez SP - 1233 EP - 42 JF - Cell JO - Cell VL - 65 IS - 7 N2 - We show that the rate of degradation of RNAI, an anti-sense repressor of the replication primer RNAII, is a key element of control in the replication of ColE1-type plasmids in vivo. Cleavage of RNAI by RNAase E, a ribosomal RNA-processing enzyme encoded or controlled by the rne (also known as ams) locus, relieves repression by endonucleolytically converting RNAI to a very rapidly decaying product, pRNAI-5. A 5' triphosphate-terminated homolog of pRNAI-5 is degraded slowly and consequently inhibits replication. Nucleotide substitutions within the RNAase E cleavage sequence alter RNAI half-life and plasmid copy number, changing also the incompatibility phenotype. RNAI variants lacking the sequence cleaved by RNAase E are eliminated by growth rate-dependent degradation, resulting in growth-responsive control of plasmid replication and copy number. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/1712252/The_rate_of_processing_and_degradation_of_antisense_RNAI_regulates_the_replication_of_ColE1_type_plasmids_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(91)90018-T DB - PRIME DP - Unbound Medicine ER -