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Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions.
Biosens Bioelectron. 2007 May 15; 22(11):2591-7.BB

Abstract

A label free optical biosensor based on a free-space Young interferometer configuration is presented. Commercial planar Ta(2) O(5) waveguides are used as sensing elements and allow the investigation of surface bound bioreactions like immunoreactions or biological affinity systems. Design criteria are discussed and a detailed characterization of the sensor performance is presented. The developed interferometer yields an effective refractive index resolution of 9 x 10(-9), corresponding to a surface coverage of approximately 13 fg/mm(2). The performance of the system is characterized by two different affinity systems: the antibody-antigen complex protein G-immunoglobulin G is used as a model system for monitoring reaction kinetics. Further measurements on a silanized surface show the formation of a streptavidin monolayer on a biotinylated surface.

Authors+Show Affiliations

Fraunhofer Institute for Physical Measurement Techniques IPM, Freiburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17125988

Citation

Schmitt, Katrin, et al. "Interferometric Biosensor Based On Planar Optical Waveguide Sensor Chips for Label-free Detection of Surface Bound Bioreactions." Biosensors & Bioelectronics, vol. 22, no. 11, 2007, pp. 2591-7.
Schmitt K, Schirmer B, Hoffmann C, et al. Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions. Biosens Bioelectron. 2007;22(11):2591-7.
Schmitt, K., Schirmer, B., Hoffmann, C., Brandenburg, A., & Meyrueis, P. (2007). Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions. Biosensors & Bioelectronics, 22(11), 2591-7.
Schmitt K, et al. Interferometric Biosensor Based On Planar Optical Waveguide Sensor Chips for Label-free Detection of Surface Bound Bioreactions. Biosens Bioelectron. 2007 May 15;22(11):2591-7. PubMed PMID: 17125988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions. AU - Schmitt,Katrin, AU - Schirmer,Bernd, AU - Hoffmann,Christian, AU - Brandenburg,Albrecht, AU - Meyrueis,Patrick, Y1 - 2006/11/27/ PY - 2006/05/17/received PY - 2006/10/02/revised PY - 2006/10/09/accepted PY - 2006/11/28/pubmed PY - 2007/6/28/medline PY - 2006/11/28/entrez SP - 2591 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 11 N2 - A label free optical biosensor based on a free-space Young interferometer configuration is presented. Commercial planar Ta(2) O(5) waveguides are used as sensing elements and allow the investigation of surface bound bioreactions like immunoreactions or biological affinity systems. Design criteria are discussed and a detailed characterization of the sensor performance is presented. The developed interferometer yields an effective refractive index resolution of 9 x 10(-9), corresponding to a surface coverage of approximately 13 fg/mm(2). The performance of the system is characterized by two different affinity systems: the antibody-antigen complex protein G-immunoglobulin G is used as a model system for monitoring reaction kinetics. Further measurements on a silanized surface show the formation of a streptavidin monolayer on a biotinylated surface. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17125988/Interferometric_biosensor_based_on_planar_optical_waveguide_sensor_chips_for_label_free_detection_of_surface_bound_bioreactions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00500-8 DB - PRIME DP - Unbound Medicine ER -