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Porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 gene expression.
Reproduction. 2006 Dec; 132(6):939-47.R

Abstract

Previous studies have suggested that the porcine endometrium may express several tissue kallikreins during the estrous cycle and early pregnancy. The present study investigated porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 mRNA expression during the estrous cycle and early pregnancy. Tissue kallikrein (KLK) gene expression was evaluated using quantitative RT-PCR and in situ hybridization. KLK1 expression was similar across the estrous cycle and early pregnancy, and localized to the endometrial luminal (L) and glandular (G) epithelium. KLK4 endometrial mRNA expression was greatest on days 0, 5, and 10 when compared with days 12, 15, and 17 of the estrous cycle and greater in cyclic compared with pregnant gilts. Expression of KLK4 was more intense in the stroma and uterine epithelium from days 0 to 10 of the estrous cycle. Endometrial KLK11 mRNA was not different between cyclic and pregnant gilts but the expression was greatest on days 10 and 12 compared with all other days evaluated. There was an increased intensity of KLK11 gene expression in the stratum compactum on day 10 of the estrous cycle and early pregnancy. Endometrial KLK14 mRNA expression was not detectable on days 5 and 10 but was expressed on days 0, 12, 15, and 17 of the estrous cycle and pregnancy. KLK14 expression was localized in the uterine L and G epithelium, and stroma throughout the endometrium after day 10. Conceptus KLK1 mRNA did not change from days 10 to 17 of gestation. However, conceptus KLK4, and 14 mRNA expression was greatest on day 10 with expression declining after day 14 of gestation. Expression of the various tissue kallikreins in the endometrium and conceptus during the estrous cycle and early pregnancy in the pig can serve in the activation of growth factors and tissue remodeling during the establishment of pregnancy.

Authors+Show Affiliations

Department of Animal Science, Oklahoma Agricultural Experiment Station, Oklahoma State University, Animal Science Building, Room 206, Stillwater, OK 74078, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17127754

Citation

Fernando, S C., et al. "Porcine Endometrial and Conceptus Tissue Kallikrein 1, 4, 11, and 14 Gene Expression." Reproduction (Cambridge, England), vol. 132, no. 6, 2006, pp. 939-47.
Fernando SC, Buck JS, Ashworth MD, et al. Porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 gene expression. Reproduction. 2006;132(6):939-47.
Fernando, S. C., Buck, J. S., Ashworth, M. D., Ross, J. W., Geisert, R. D., & DeSilva, U. (2006). Porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 gene expression. Reproduction (Cambridge, England), 132(6), 939-47.
Fernando SC, et al. Porcine Endometrial and Conceptus Tissue Kallikrein 1, 4, 11, and 14 Gene Expression. Reproduction. 2006;132(6):939-47. PubMed PMID: 17127754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 gene expression. AU - Fernando,S C, AU - Buck,J S, AU - Ashworth,M D, AU - Ross,J W, AU - Geisert,R D, AU - DeSilva,U, PY - 2006/11/28/pubmed PY - 2007/6/20/medline PY - 2006/11/28/entrez SP - 939 EP - 47 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 132 IS - 6 N2 - Previous studies have suggested that the porcine endometrium may express several tissue kallikreins during the estrous cycle and early pregnancy. The present study investigated porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 mRNA expression during the estrous cycle and early pregnancy. Tissue kallikrein (KLK) gene expression was evaluated using quantitative RT-PCR and in situ hybridization. KLK1 expression was similar across the estrous cycle and early pregnancy, and localized to the endometrial luminal (L) and glandular (G) epithelium. KLK4 endometrial mRNA expression was greatest on days 0, 5, and 10 when compared with days 12, 15, and 17 of the estrous cycle and greater in cyclic compared with pregnant gilts. Expression of KLK4 was more intense in the stroma and uterine epithelium from days 0 to 10 of the estrous cycle. Endometrial KLK11 mRNA was not different between cyclic and pregnant gilts but the expression was greatest on days 10 and 12 compared with all other days evaluated. There was an increased intensity of KLK11 gene expression in the stratum compactum on day 10 of the estrous cycle and early pregnancy. Endometrial KLK14 mRNA expression was not detectable on days 5 and 10 but was expressed on days 0, 12, 15, and 17 of the estrous cycle and pregnancy. KLK14 expression was localized in the uterine L and G epithelium, and stroma throughout the endometrium after day 10. Conceptus KLK1 mRNA did not change from days 10 to 17 of gestation. However, conceptus KLK4, and 14 mRNA expression was greatest on day 10 with expression declining after day 14 of gestation. Expression of the various tissue kallikreins in the endometrium and conceptus during the estrous cycle and early pregnancy in the pig can serve in the activation of growth factors and tissue remodeling during the establishment of pregnancy. SN - 1470-1626 UR - https://www.unboundmedicine.com/medline/citation/17127754/Porcine_endometrial_and_conceptus_tissue_kallikrein_1_4_11_and_14_gene_expression_ L2 - https://rep.bioscientifica.com/doi/10.1530/rep.1.01013 DB - PRIME DP - Unbound Medicine ER -