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Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids.
Int J Obes (Lond). 2007 Jun; 31(6):1004-13.IJ

Abstract

OBJECTIVE

Obesity is associated with reduced insulin sensitivity and extensive reorganization of adipose tissue. As polyunsaturated fatty acids (PUFA) appear to inhibit diabetes development, we investigated PUFA effects on markers of matrix remodeling in white adipose tissue.

METHODS AND PROCEDURE

Male obese diabetic (db/db) mice were treated with either a low-fat standard diet (LF), or high-fat diets rich in saturated and monounsaturated fatty acids (HF/S), n-6 PUFA (HF/6) or the latter including marine n-3 PUFA (HF/3). White adipose tissue was analyzed for gene expression, fatty acid composition and by immunofluorescence.

RESULTS

HF/S treatment increased adipose tissue expression of a number of genes involved in matrix degradation including matrix metalloproteinase (MMP)-12, -14 and cathepsin K, L and S compared with LF. MMP-12 gene was expressed in macrophages and adipocytes, and MMP-12 protein colocalized with both cell types. In addition, mean adipocyte area increased by 1.6-fold in HF/S-treated mice. Genes essential for collagen production, such as procollagen I, III, VI, tenascin C and biglycan were upregulated in HF/S-treated animals as well. N-3 PUFA supplementation resulted in enrichment of these fatty acids in adipose tissue. Moreover, n-3 PUFA inhibited the HF/S-induced upregulation of genes involved in matrix degradation and production I restored mean adipocyte area and prevented MMP-12 expression in macrophages and adipocytes.

CONCLUSION

N-3 PUFA prevent high-fat diet-induced matrix remodeling and adipocyte enlargement in adipose tissue of obese diabetic mice. Such changes could contribute to diabetes prevention by n-3 PUFA in obese patients.

Authors+Show Affiliations

Clinical Division of Endocrinology and Metabolism, Department of Internal Medicine III, Medical University Vienna, Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17130847

Citation

Huber, J, et al. "Prevention of High-fat Diet-induced Adipose Tissue Remodeling in Obese Diabetic Mice By N-3 Polyunsaturated Fatty Acids." International Journal of Obesity (2005), vol. 31, no. 6, 2007, pp. 1004-13.
Huber J, Löffler M, Bilban M, et al. Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids. Int J Obes (Lond). 2007;31(6):1004-13.
Huber, J., Löffler, M., Bilban, M., Reimers, M., Kadl, A., Todoric, J., Zeyda, M., Geyeregger, R., Schreiner, M., Weichhart, T., Leitinger, N., Waldhäusl, W., & Stulnig, T. M. (2007). Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids. International Journal of Obesity (2005), 31(6), 1004-13.
Huber J, et al. Prevention of High-fat Diet-induced Adipose Tissue Remodeling in Obese Diabetic Mice By N-3 Polyunsaturated Fatty Acids. Int J Obes (Lond). 2007;31(6):1004-13. PubMed PMID: 17130847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids. AU - Huber,J, AU - Löffler,M, AU - Bilban,M, AU - Reimers,M, AU - Kadl,A, AU - Todoric,J, AU - Zeyda,M, AU - Geyeregger,R, AU - Schreiner,M, AU - Weichhart,T, AU - Leitinger,N, AU - Waldhäusl,W, AU - Stulnig,T M, Y1 - 2006/11/28/ PY - 2006/11/30/pubmed PY - 2007/8/19/medline PY - 2006/11/30/entrez SP - 1004 EP - 13 JF - International journal of obesity (2005) JO - Int J Obes (Lond) VL - 31 IS - 6 N2 - OBJECTIVE: Obesity is associated with reduced insulin sensitivity and extensive reorganization of adipose tissue. As polyunsaturated fatty acids (PUFA) appear to inhibit diabetes development, we investigated PUFA effects on markers of matrix remodeling in white adipose tissue. METHODS AND PROCEDURE: Male obese diabetic (db/db) mice were treated with either a low-fat standard diet (LF), or high-fat diets rich in saturated and monounsaturated fatty acids (HF/S), n-6 PUFA (HF/6) or the latter including marine n-3 PUFA (HF/3). White adipose tissue was analyzed for gene expression, fatty acid composition and by immunofluorescence. RESULTS: HF/S treatment increased adipose tissue expression of a number of genes involved in matrix degradation including matrix metalloproteinase (MMP)-12, -14 and cathepsin K, L and S compared with LF. MMP-12 gene was expressed in macrophages and adipocytes, and MMP-12 protein colocalized with both cell types. In addition, mean adipocyte area increased by 1.6-fold in HF/S-treated mice. Genes essential for collagen production, such as procollagen I, III, VI, tenascin C and biglycan were upregulated in HF/S-treated animals as well. N-3 PUFA supplementation resulted in enrichment of these fatty acids in adipose tissue. Moreover, n-3 PUFA inhibited the HF/S-induced upregulation of genes involved in matrix degradation and production I restored mean adipocyte area and prevented MMP-12 expression in macrophages and adipocytes. CONCLUSION: N-3 PUFA prevent high-fat diet-induced matrix remodeling and adipocyte enlargement in adipose tissue of obese diabetic mice. Such changes could contribute to diabetes prevention by n-3 PUFA in obese patients. SN - 0307-0565 UR - https://www.unboundmedicine.com/medline/citation/17130847/Prevention_of_high_fat_diet_induced_adipose_tissue_remodeling_in_obese_diabetic_mice_by_n_3_polyunsaturated_fatty_acids_ L2 - https://doi.org/10.1038/sj.ijo.0803511 DB - PRIME DP - Unbound Medicine ER -