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[Genotypying of clinical isolates of Helicobacter pylori by cagA, vacA and babA2 virulence associated genes. First detection of a babA2 positive strain in Chilean patients].
Rev Med Chil. 2006 Aug; 134(8):981-8.RM

Abstract

BACKGROUND

Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and gastric cancer has been associated to cagA+, vacAs1 and babA2+ H pylori strains. Gene babA2 has not yet been described in clinical isolates from Chilean patients.

AIM

To investigate the presence of cagA, vacA (s and m) and babA2 genes in clinical isolates of H pylori from Chilean patients.

MATERIAL AND METHODS

Sixty six isolates from 41 patients were genotyped by PCR, using primers for s1a, s1b, s2, m1, m2, cagA and babA2 genes as previously described.

RESULTS

cagA gene was detected in 16 isolates (24.2%) while vacAs1a, vacAs1b, vacAs2, vacAm1 and vacAm2 were detected in 28 (42.4%), 14 (21.2%), 17 (25.8%), 21 (31.8%) and 29 isolates (43.9%), respectively. One isolate (1.5%) was babA2 positive, being the first isolate with this genotype described in Chile. Besides the babA2+ genotype this clinical isolate also presented cagA+ and vacAs1a which has been related with metaplasia or gastric cancer. Five isolates showed an ulcerogenic profile cagA+, vacAs1m1.

CONCLUSIONS

The results presented indicate the prevalence of vacAs1m1 genotype among the clinical isolates analyzed, and a low frequency of babA2 genotype.

Authors+Show Affiliations

Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile. apgarcia@udec.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

spa

PubMed ID

17130985

Citation

García, Apolinaria, et al. "[Genotypying of Clinical Isolates of Helicobacter Pylori By cagA, vacA and babA2 Virulence Associated Genes. First Detection of a babA2 Positive Strain in Chilean Patients]." Revista Medica De Chile, vol. 134, no. 8, 2006, pp. 981-8.
García A, Barra R, Delgado C, et al. [Genotypying of clinical isolates of Helicobacter pylori by cagA, vacA and babA2 virulence associated genes. First detection of a babA2 positive strain in Chilean patients]. Rev Med Chil. 2006;134(8):981-8.
García, A., Barra, R., Delgado, C., Kawaguchi, F., Trabal, N., Montenegro, S., & González, C. (2006). [Genotypying of clinical isolates of Helicobacter pylori by cagA, vacA and babA2 virulence associated genes. First detection of a babA2 positive strain in Chilean patients]. Revista Medica De Chile, 134(8), 981-8.
García A, et al. [Genotypying of Clinical Isolates of Helicobacter Pylori By cagA, vacA and babA2 Virulence Associated Genes. First Detection of a babA2 Positive Strain in Chilean Patients]. Rev Med Chil. 2006;134(8):981-8. PubMed PMID: 17130985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Genotypying of clinical isolates of Helicobacter pylori by cagA, vacA and babA2 virulence associated genes. First detection of a babA2 positive strain in Chilean patients]. AU - García,Apolinaria, AU - Barra,Ricardo, AU - Delgado,Carolina, AU - Kawaguchi,Fernando, AU - Trabal,Natalia, AU - Montenegro,Sonia, AU - González,Carlos, Y1 - 2006/11/14/ PY - 2006/11/30/pubmed PY - 2007/6/19/medline PY - 2006/11/30/entrez SP - 981 EP - 8 JF - Revista medica de Chile JO - Rev Med Chil VL - 134 IS - 8 N2 - BACKGROUND: Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and gastric cancer has been associated to cagA+, vacAs1 and babA2+ H pylori strains. Gene babA2 has not yet been described in clinical isolates from Chilean patients. AIM: To investigate the presence of cagA, vacA (s and m) and babA2 genes in clinical isolates of H pylori from Chilean patients. MATERIAL AND METHODS: Sixty six isolates from 41 patients were genotyped by PCR, using primers for s1a, s1b, s2, m1, m2, cagA and babA2 genes as previously described. RESULTS: cagA gene was detected in 16 isolates (24.2%) while vacAs1a, vacAs1b, vacAs2, vacAm1 and vacAm2 were detected in 28 (42.4%), 14 (21.2%), 17 (25.8%), 21 (31.8%) and 29 isolates (43.9%), respectively. One isolate (1.5%) was babA2 positive, being the first isolate with this genotype described in Chile. Besides the babA2+ genotype this clinical isolate also presented cagA+ and vacAs1a which has been related with metaplasia or gastric cancer. Five isolates showed an ulcerogenic profile cagA+, vacAs1m1. CONCLUSIONS: The results presented indicate the prevalence of vacAs1m1 genotype among the clinical isolates analyzed, and a low frequency of babA2 genotype. SN - 0034-9887 UR - https://www.unboundmedicine.com/medline/citation/17130985/[Genotypying_of_clinical_isolates_of_Helicobacter_pylori_by_cagA_vacA_and_babA2_virulence_associated_genes__First_detection_of_a_babA2_positive_strain_in_Chilean_patients]_ L2 - http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000800006&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -