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Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings.
J Exp Bot. 2007; 58(2):253-65.JE

Abstract

The impacts of drought on plant growth and development limit cereal crop production worldwide. Rice (Oryza sativa) productivity and production is severely affected due to recurrent droughts in almost all agroecological zones. With the advent of molecular and genomic technologies, emphasis is now placed on understanding the mechanisms of genetic control of the drought-stress response. In order to identify genes associated with water-stress response in rice, ESTs generated from a normalized cDNA library, constructed from drought-stressed leaf tissue of an indica cultivar, Nagina 22 were used. Analysis of 7794 cDNA sequences led to the identification of 5815 rice ESTs. Of these, 334 exhibited no significant sequence homology with any rice ESTs or full-length cDNAs in public databases, indicating that these transcripts are enriched during drought stress. Analysis of these 5815 ESTs led to the identification of 1677 unique sequences. To characterize this drought transcriptome further and to identify candidate genes associated with the drought-stress response, the rice data were compared with those for abiotic stress-induced sequences obtained from expression profiling studies in Arabidopsis, barley, maize, and rice. This comparative analysis identified 589 putative stress-responsive genes (SRGs) that are shared by these diverse plant species. Further, the identified leaf SRGs were compared to expression profiles for a drought-stressed rice panicle library to identify common sequences. Significantly, 125 genes were found to be expressed under drought stress in both tissues. The functional classification of these 125 genes showed that a majority of them are associated with cellular metabolism, signal transduction, and transcriptional regulation.

Authors+Show Affiliations

Gorantla M
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad-500046, AP, India.
Babu PR
No affiliation info available
Lachagari VB
No affiliation info available
Reddy AM
No affiliation info available
Wusirika R
No affiliation info available
Bennetzen JL
No affiliation info available
Reddy AR
No affiliation info available

MeSH

Adaptation, PhysiologicalDisastersExpressed Sequence TagsGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantOryzaSeedlingsWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17132712

Citation

Gorantla, Markandeya, et al. "Identification of Stress-responsive Genes in an Indica Rice (Oryza Sativa L.) Using ESTs Generated From Drought-stressed Seedlings." Journal of Experimental Botany, vol. 58, no. 2, 2007, pp. 253-65.
Gorantla M, Babu PR, Lachagari VB, et al. Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings. J Exp Bot. 2007;58(2):253-65.
Gorantla, M., Babu, P. R., Lachagari, V. B., Reddy, A. M., Wusirika, R., Bennetzen, J. L., & Reddy, A. R. (2007). Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings. Journal of Experimental Botany, 58(2), 253-65.
Gorantla M, et al. Identification of Stress-responsive Genes in an Indica Rice (Oryza Sativa L.) Using ESTs Generated From Drought-stressed Seedlings. J Exp Bot. 2007;58(2):253-65. PubMed PMID: 17132712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings. AU - Gorantla,Markandeya, AU - Babu,P R, AU - Lachagari,V B Reddy, AU - Reddy,A M M, AU - Wusirika,Ramakrishna, AU - Bennetzen,Jeffrey L, AU - Reddy,Arjula R, Y1 - 2006/11/28/ PY - 2006/11/30/pubmed PY - 2007/4/6/medline PY - 2006/11/30/entrez SP - 253 EP - 65 JF - Journal of experimental botany JO - J Exp Bot VL - 58 IS - 2 N2 - The impacts of drought on plant growth and development limit cereal crop production worldwide. Rice (Oryza sativa) productivity and production is severely affected due to recurrent droughts in almost all agroecological zones. With the advent of molecular and genomic technologies, emphasis is now placed on understanding the mechanisms of genetic control of the drought-stress response. In order to identify genes associated with water-stress response in rice, ESTs generated from a normalized cDNA library, constructed from drought-stressed leaf tissue of an indica cultivar, Nagina 22 were used. Analysis of 7794 cDNA sequences led to the identification of 5815 rice ESTs. Of these, 334 exhibited no significant sequence homology with any rice ESTs or full-length cDNAs in public databases, indicating that these transcripts are enriched during drought stress. Analysis of these 5815 ESTs led to the identification of 1677 unique sequences. To characterize this drought transcriptome further and to identify candidate genes associated with the drought-stress response, the rice data were compared with those for abiotic stress-induced sequences obtained from expression profiling studies in Arabidopsis, barley, maize, and rice. This comparative analysis identified 589 putative stress-responsive genes (SRGs) that are shared by these diverse plant species. Further, the identified leaf SRGs were compared to expression profiles for a drought-stressed rice panicle library to identify common sequences. Significantly, 125 genes were found to be expressed under drought stress in both tissues. The functional classification of these 125 genes showed that a majority of them are associated with cellular metabolism, signal transduction, and transcriptional regulation. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/17132712/Identification_of_stress_responsive_genes_in_an_indica_rice__Oryza_sativa_L___using_ESTs_generated_from_drought_stressed_seedlings_ DB - PRIME DP - Unbound Medicine ER -