Tags

Type your tag names separated by a space and hit enter

LIF detection of peptides and proteins in CE.
Electrophoresis. 2007 Jan; 28(1-2):208-32.E

Abstract

CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005.

Authors+Show Affiliations

García-Campaña AM
Department of Analytical Chemistry, University of Granada, Granada, Spain. amgarcia@ugr.es
Taverna M
No affiliation info available
Fabre H
No affiliation info available

MeSH

Electrophoresis, CapillaryFluorescent DyesFluorometryLasersLuminescent ProteinsPeptidesProteins

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

17136735

Citation

García-Campaña, Ana M., et al. "LIF Detection of Peptides and Proteins in CE." Electrophoresis, vol. 28, no. 1-2, 2007, pp. 208-32.
García-Campaña AM, Taverna M, Fabre H. LIF detection of peptides and proteins in CE. Electrophoresis. 2007;28(1-2):208-32.
García-Campaña, A. M., Taverna, M., & Fabre, H. (2007). LIF detection of peptides and proteins in CE. Electrophoresis, 28(1-2), 208-32.
García-Campaña AM, Taverna M, Fabre H. LIF Detection of Peptides and Proteins in CE. Electrophoresis. 2007;28(1-2):208-32. PubMed PMID: 17136735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LIF detection of peptides and proteins in CE. AU - García-Campaña,Ana M, AU - Taverna,Myriam, AU - Fabre,Huguette, PY - 2006/12/1/pubmed PY - 2007/3/3/medline PY - 2006/12/1/entrez SP - 208 EP - 32 JF - Electrophoresis JO - Electrophoresis VL - 28 IS - 1-2 N2 - CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17136735/LIF_detection_of_peptides_and_proteins_in_CE_ DB - PRIME DP - Unbound Medicine ER -