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Effect of the carboxylic ionophore monensin on histamine release from rat peritoneal mast cells.
Agents Actions. 1991 Mar; 32(3-4):253-60.AA

Abstract

The effect of the monovalent carboxylic ionophore monensin, which mediates a one-for-one exchange of intracellular H+ for extracellular Na+, was investigated in purified rat peritoneal mast cells. Monensin inhibited histamine secretion induced by compound 48/80, adriamycin and the calcium ionophore A23187; the inhibitory effect was maximal when the compound was added at least 10 min before the secretagogues. Washing of cells before addition of the secretagogues did not abolish the inhibitory effect of monensin. On the contrary the carboxylic ionophore was completely ineffective in preventing concanavalin A-induced histamine release. When rat peritoneal mast cells were incubated in the presence of monensin for longer period (up to 5 hours), the substance induced a slow, progressive and dose dependent histamine release, which, at least for lower doses was noncytotoxic. The secretory effect of monensin was still present if the ionophore was washed away after 10 min of incubation, and the incubation continued in drug-free medium. Monensin stimulated histamine secretion was strictly dependent on extracellular Na+ concentrations, and independent on extracellular Ca++.

Authors+Show Affiliations

Decorti G
Institute of Pharmacology, School of Medicine, University of Trieste, Italy.
Bartoli Klugmann F
No affiliation info available
Crivellato E
No affiliation info available
Mallardi F
No affiliation info available
Candussio L
No affiliation info available
Baldini L
No affiliation info available

MeSH

AnimalsCalcimycinCalciumConcanavalin ACytoplasmic GranulesDose-Response Relationship, DrugDoxorubicinHistamine ReleaseMast CellsMonensinPeritoneal CavityRatsRats, Inbred StrainsSodiump-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1713737

Citation

Decorti, G, et al. "Effect of the Carboxylic Ionophore Monensin On Histamine Release From Rat Peritoneal Mast Cells." Agents and Actions, vol. 32, no. 3-4, 1991, pp. 253-60.
Decorti G, Bartoli Klugmann F, Crivellato E, et al. Effect of the carboxylic ionophore monensin on histamine release from rat peritoneal mast cells. Agents Actions. 1991;32(3-4):253-60.
Decorti, G., Bartoli Klugmann, F., Crivellato, E., Mallardi, F., Candussio, L., & Baldini, L. (1991). Effect of the carboxylic ionophore monensin on histamine release from rat peritoneal mast cells. Agents and Actions, 32(3-4), 253-60.
Decorti G, et al. Effect of the Carboxylic Ionophore Monensin On Histamine Release From Rat Peritoneal Mast Cells. Agents Actions. 1991;32(3-4):253-60. PubMed PMID: 1713737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of the carboxylic ionophore monensin on histamine release from rat peritoneal mast cells. AU - Decorti,G, AU - Bartoli Klugmann,F, AU - Crivellato,E, AU - Mallardi,F, AU - Candussio,L, AU - Baldini,L, PY - 1991/3/1/pubmed PY - 1991/3/1/medline PY - 1991/3/1/entrez SP - 253 EP - 60 JF - Agents and actions JO - Agents Actions VL - 32 IS - 3-4 N2 - The effect of the monovalent carboxylic ionophore monensin, which mediates a one-for-one exchange of intracellular H+ for extracellular Na+, was investigated in purified rat peritoneal mast cells. Monensin inhibited histamine secretion induced by compound 48/80, adriamycin and the calcium ionophore A23187; the inhibitory effect was maximal when the compound was added at least 10 min before the secretagogues. Washing of cells before addition of the secretagogues did not abolish the inhibitory effect of monensin. On the contrary the carboxylic ionophore was completely ineffective in preventing concanavalin A-induced histamine release. When rat peritoneal mast cells were incubated in the presence of monensin for longer period (up to 5 hours), the substance induced a slow, progressive and dose dependent histamine release, which, at least for lower doses was noncytotoxic. The secretory effect of monensin was still present if the ionophore was washed away after 10 min of incubation, and the incubation continued in drug-free medium. Monensin stimulated histamine secretion was strictly dependent on extracellular Na+ concentrations, and independent on extracellular Ca++. SN - 0065-4299 UR - https://www.unboundmedicine.com/medline/citation/1713737/Effect_of_the_carboxylic_ionophore_monensin_on_histamine_release_from_rat_peritoneal_mast_cells_ DB - PRIME DP - Unbound Medicine ER -