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[Development of Dasypyrum genome specific marker by using wheat microsatellites].
Yi Chuan. 2006 Dec; 28(12):1573-9.YC

Abstract

One hundred and two SSR primer pairs, distributed in chromosome 1A to 7A, 1B to 7B, 1D to 7D of Triticum aestivum, were investigated on Dasypyrum breviaristatum, D.villosum, wheat-Dasypyrum amphiploids and its derivatives, with the control of common wheat Chinese Spring and elite wheat cultivars. A specific polymorphic DNA fragment of about 400 bp (415 bp-long by sequenced, named Xgwm301/415) amplified by primer pair Xgwm301 was obtained in all lines containing Dasypyrum chromosomes, but there were not the case in the tested common wheat. Furthermore, PCR analysis was performed on a set of T. aestivum-Dasypyrum addition, the result showed that all the seven pairs of villosum chromosomes contain Xgwm301/415. Therefore, Xgwm301/415 is a genome-specific polymorphic DNA segment for genera of Dasypyrum, and it could be used as a molecular marker for detection of chromosomes of Dasypyrum in wheat.

Authors+Show Affiliations

Liu C
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. lch6688407@163.com
Yang ZJ
No affiliation info available
Feng J
No affiliation info available
Zhou JP
No affiliation info available
Chi SH
No affiliation info available
Ren ZL
No affiliation info available

MeSH

Base SequenceChromosomes, PlantDNA, PlantGenetic MarkersGenome, PlantMicrosatellite RepeatsMolecular Sequence DataPolymerase Chain ReactionPolymorphism, GeneticTemperatureTriticum

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17138545

Citation

Liu, Cheng, et al. "[Development of Dasypyrum Genome Specific Marker By Using Wheat Microsatellites]." Yi Chuan = Hereditas, vol. 28, no. 12, 2006, pp. 1573-9.
Liu C, Yang ZJ, Feng J, et al. [Development of Dasypyrum genome specific marker by using wheat microsatellites]. Yi Chuan. 2006;28(12):1573-9.
Liu, C., Yang, Z. J., Feng, J., Zhou, J. P., Chi, S. H., & Ren, Z. L. (2006). [Development of Dasypyrum genome specific marker by using wheat microsatellites]. Yi Chuan = Hereditas, 28(12), 1573-9.
Liu C, et al. [Development of Dasypyrum Genome Specific Marker By Using Wheat Microsatellites]. Yi Chuan. 2006;28(12):1573-9. PubMed PMID: 17138545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Development of Dasypyrum genome specific marker by using wheat microsatellites]. AU - Liu,Cheng, AU - Yang,Zu-June, AU - Feng,Juan, AU - Zhou,Jian-Ping, AU - Chi,Shi-Hua, AU - Ren,Zheng-Long, PY - 2006/12/2/pubmed PY - 2009/4/18/medline PY - 2006/12/2/entrez SP - 1573 EP - 9 JF - Yi chuan = Hereditas JO - Yi Chuan VL - 28 IS - 12 N2 - One hundred and two SSR primer pairs, distributed in chromosome 1A to 7A, 1B to 7B, 1D to 7D of Triticum aestivum, were investigated on Dasypyrum breviaristatum, D.villosum, wheat-Dasypyrum amphiploids and its derivatives, with the control of common wheat Chinese Spring and elite wheat cultivars. A specific polymorphic DNA fragment of about 400 bp (415 bp-long by sequenced, named Xgwm301/415) amplified by primer pair Xgwm301 was obtained in all lines containing Dasypyrum chromosomes, but there were not the case in the tested common wheat. Furthermore, PCR analysis was performed on a set of T. aestivum-Dasypyrum addition, the result showed that all the seven pairs of villosum chromosomes contain Xgwm301/415. Therefore, Xgwm301/415 is a genome-specific polymorphic DNA segment for genera of Dasypyrum, and it could be used as a molecular marker for detection of chromosomes of Dasypyrum in wheat. SN - 0253-9772 UR - https://www.unboundmedicine.com/medline/citation/17138545/[Development_of_Dasypyrum_genome_specific_marker_by_using_wheat_microsatellites]_ DB - PRIME DP - Unbound Medicine ER -