Tags

Type your tag names separated by a space and hit enter

Inorganic polyphosphates and exopolyphosphatases in different cell compartments of Saccharomyces cerevisiae.
Biochemistry (Mosc). 2006 Nov; 71(11):1171-5.B

Abstract

The cytosol, nuclei, vacuoles, and mitochondria of the yeast Saccharomyces cerevisiae possess inorganic polyphosphates (polyPs). PolyP levels, spectra of polyP chain lengths, and their dependence on the growth phase are distinguished in the mentioned compartments. Inactivation of the PPX1 gene has no effect on the polyP metabolism under cultivation of the yeast in medium with glucose and 5-7 mM P(i). Inactivation of the PPN1 gene results in elimination of the high-molecular-mass exopolyphosphatases (approximately 120 to 830 kD) of the cytosol, nuclei, vacuoles, and mitochondria of S. cerevisiae suggesting that it is just PPN1 that encodes these enzymes. Expression of the low-molecular-mass exopolyphosphatase of approximately 45 kD encoded by the PPX1 gene decreases under PPN1 inactivation as well. While PPN1 inactivation has negligible effect on polyP levels, it results in increase in the long-chain polyPs in all the compartments under study.

Links

Aggregator Full Text

Authors+Show Affiliations

Lichko LP
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia. alla@ibpm.pushchino.ru
Kulakovskaya TV
No affiliation info available
Kulaev IS
No affiliation info available

MeSH

Acid Anhydride HydrolasesCell CompartmentationPhosphatesPolyphosphatesSaccharomyces cerevisiaeTissue Distribution

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

17140377

Citation

Lichko, L P., et al. "Inorganic Polyphosphates and Exopolyphosphatases in Different Cell Compartments of Saccharomyces Cerevisiae." Biochemistry. Biokhimiia, vol. 71, no. 11, 2006, pp. 1171-5.
Lichko LP, Kulakovskaya TV, Kulaev IS. Inorganic polyphosphates and exopolyphosphatases in different cell compartments of Saccharomyces cerevisiae. Biochemistry (Mosc). 2006;71(11):1171-5.
Lichko, L. P., Kulakovskaya, T. V., & Kulaev, I. S. (2006). Inorganic polyphosphates and exopolyphosphatases in different cell compartments of Saccharomyces cerevisiae. Biochemistry. Biokhimiia, 71(11), 1171-5.
Lichko LP, Kulakovskaya TV, Kulaev IS. Inorganic Polyphosphates and Exopolyphosphatases in Different Cell Compartments of Saccharomyces Cerevisiae. Biochemistry (Mosc). 2006;71(11):1171-5. PubMed PMID: 17140377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inorganic polyphosphates and exopolyphosphatases in different cell compartments of Saccharomyces cerevisiae. AU - Lichko,L P, AU - Kulakovskaya,T V, AU - Kulaev,I S, PY - 2006/12/5/pubmed PY - 2007/5/1/medline PY - 2006/12/5/entrez SP - 1171 EP - 5 JF - Biochemistry. Biokhimiia JO - Biochemistry (Mosc) VL - 71 IS - 11 N2 - The cytosol, nuclei, vacuoles, and mitochondria of the yeast Saccharomyces cerevisiae possess inorganic polyphosphates (polyPs). PolyP levels, spectra of polyP chain lengths, and their dependence on the growth phase are distinguished in the mentioned compartments. Inactivation of the PPX1 gene has no effect on the polyP metabolism under cultivation of the yeast in medium with glucose and 5-7 mM P(i). Inactivation of the PPN1 gene results in elimination of the high-molecular-mass exopolyphosphatases (approximately 120 to 830 kD) of the cytosol, nuclei, vacuoles, and mitochondria of S. cerevisiae suggesting that it is just PPN1 that encodes these enzymes. Expression of the low-molecular-mass exopolyphosphatase of approximately 45 kD encoded by the PPX1 gene decreases under PPN1 inactivation as well. While PPN1 inactivation has negligible effect on polyP levels, it results in increase in the long-chain polyPs in all the compartments under study. SN - 0006-2979 UR - https://www.unboundmedicine.com/medline/citation/17140377/Inorganic_polyphosphates_and_exopolyphosphatases_in_different_cell_compartments_of_Saccharomyces_cerevisiae_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=17140377.ui DB - PRIME DP - Unbound Medicine ER -