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Protective effect of Didymocarpus pedicellata on ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and hyperproliferative response.
Chem Biol Interact. 2007 Jan 05; 165(1):33-44.CB

Abstract

Didymocarpus pedicellata R. Br. (Gesneriaceae) is widely used in traditional Indian medicines against renal afflictions. In the present study, we have revealed ethanolic extract of aerial parts of D. pedicellata to possess significant antioxidant activity and protect against ferric nitrilotriacetate (Fe-NTA) mediated renal oxidative stress, nephrotoxicity and tumor promotion response. D. pedicellata extract was found to possess a high content of total polyphenolics, exhibit potent reducing power and significantly scavenge free radicals including several reactive oxygen species (ROS) and reactive nitrogen species (RNS). The extract also significantly and dose-dependently protected against Fe-NTA plus H(2)O(2)-mediated damage to lipids and DNA. Protective efficacy of the extract was also tested in vivo against Fe-NTA mediated nephrotoxicity and tumor promotion response. Administration of Fe-NTA (9 mg/kg body weight, i.p.) to Swiss albino mice depleted renal glutathione content and activities of antioxidant and phase II metabolizing enzymes with concomitant induction of oxidative damage. Fe-NTA also incited hyperproliferation response elevating ornithine decarboxylase activity and [(3)H]-thymidine incorporation into DNA. Elevation in serum creatinine (SCr) and blood urea nitrogen (BUN), and histopathological changes were also evident and suggested Fe-NTA to afflict damage to kidney. Pretreatment of mice with D. pedicellata extract (100-200 mg/kg body weight) for 7 days not only restored antioxidant armory near normal values but also significantly protected against renal oxidative stress and damage restoring normal renal architecture and levels of renal damage markers, viz., BUN and SCr. The results of the present study indicate D. pedicellata to possess potent antioxidant and free radical scavenging activities and preclude oxidative damage and hyperproliferation in renal tissues.

Authors+Show Affiliations

Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17140554

Citation

Kaur, Gurpreet, et al. "Protective Effect of Didymocarpus Pedicellata On Ferric Nitrilotriacetate (Fe-NTA) Induced Renal Oxidative Stress and Hyperproliferative Response." Chemico-biological Interactions, vol. 165, no. 1, 2007, pp. 33-44.
Kaur G, Lone IA, Athar M, et al. Protective effect of Didymocarpus pedicellata on ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and hyperproliferative response. Chem Biol Interact. 2007;165(1):33-44.
Kaur, G., Lone, I. A., Athar, M., & Alam, M. S. (2007). Protective effect of Didymocarpus pedicellata on ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and hyperproliferative response. Chemico-biological Interactions, 165(1), 33-44.
Kaur G, et al. Protective Effect of Didymocarpus Pedicellata On Ferric Nitrilotriacetate (Fe-NTA) Induced Renal Oxidative Stress and Hyperproliferative Response. Chem Biol Interact. 2007 Jan 5;165(1):33-44. PubMed PMID: 17140554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of Didymocarpus pedicellata on ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and hyperproliferative response. AU - Kaur,Gurpreet, AU - Lone,Irshad A, AU - Athar,Mohammad, AU - Alam,M Sarwar, Y1 - 2006/11/02/ PY - 2006/08/09/received PY - 2006/10/19/revised PY - 2006/10/20/accepted PY - 2006/12/5/pubmed PY - 2007/3/7/medline PY - 2006/12/5/entrez SP - 33 EP - 44 JF - Chemico-biological interactions JO - Chem. Biol. Interact. VL - 165 IS - 1 N2 - Didymocarpus pedicellata R. Br. (Gesneriaceae) is widely used in traditional Indian medicines against renal afflictions. In the present study, we have revealed ethanolic extract of aerial parts of D. pedicellata to possess significant antioxidant activity and protect against ferric nitrilotriacetate (Fe-NTA) mediated renal oxidative stress, nephrotoxicity and tumor promotion response. D. pedicellata extract was found to possess a high content of total polyphenolics, exhibit potent reducing power and significantly scavenge free radicals including several reactive oxygen species (ROS) and reactive nitrogen species (RNS). The extract also significantly and dose-dependently protected against Fe-NTA plus H(2)O(2)-mediated damage to lipids and DNA. Protective efficacy of the extract was also tested in vivo against Fe-NTA mediated nephrotoxicity and tumor promotion response. Administration of Fe-NTA (9 mg/kg body weight, i.p.) to Swiss albino mice depleted renal glutathione content and activities of antioxidant and phase II metabolizing enzymes with concomitant induction of oxidative damage. Fe-NTA also incited hyperproliferation response elevating ornithine decarboxylase activity and [(3)H]-thymidine incorporation into DNA. Elevation in serum creatinine (SCr) and blood urea nitrogen (BUN), and histopathological changes were also evident and suggested Fe-NTA to afflict damage to kidney. Pretreatment of mice with D. pedicellata extract (100-200 mg/kg body weight) for 7 days not only restored antioxidant armory near normal values but also significantly protected against renal oxidative stress and damage restoring normal renal architecture and levels of renal damage markers, viz., BUN and SCr. The results of the present study indicate D. pedicellata to possess potent antioxidant and free radical scavenging activities and preclude oxidative damage and hyperproliferation in renal tissues. SN - 0009-2797 UR - https://www.unboundmedicine.com/medline/citation/17140554/Protective_effect_of_Didymocarpus_pedicellata_on_ferric_nitrilotriacetate__Fe_NTA__induced_renal_oxidative_stress_and_hyperproliferative_response_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(06)00308-5 DB - PRIME DP - Unbound Medicine ER -