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Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection.
J Virol Methods. 2007 Mar; 140(1-2):75-9.JV

Abstract

A commercial dengue NS1 antigen-capture ELISA was evaluated to demonstrate its potential application for early laboratory diagnosis of acute dengue virus infection. Dengue virus NS1 antigen was detected in 199 of 213 acute serum samples from patients with laboratory confirmation of acute dengue virus infection but none of the 354 healthy blood donors' serum specimens. The dengue NS1 antigen-capture ELISA gave an overall sensitivity of 93.4% (199/213) and a specificity of 100% (354/354). The sensitivity was significantly higher in acute primary dengue (97.3%) than in acute secondary dengue (70.0%). The positive predictive value of the dengue NS1 antigen-capture ELISA was 100% and negative predictive value was 97.3%. Comparatively, virus isolation gave an overall positive isolation rate of 68.1% with a positive isolation rate of 73.9 and 31.0% for acute primary dengue and acute secondary dengue, respectively. Molecular detection of dengue RNA by RT-PCR gave an overall positive detection rate of 66.7% with a detection rate of 65.2 and 75.9% for acute primary dengue and acute secondary dengue, respectively. The results indicate that the commercial dengue NS1 antigen-capture ELISA may be superior to virus isolation and RT-PCR for the laboratory diagnosis of acute dengue infection based on a single serum sample.

Authors+Show Affiliations

Makmal Kesihatan Awam Kebangsaan (National Public Health Laboratory), Kementerian Kesihatan, Lot 1853 Kg. Melayu, 47000 Sungai Buloh, Selangor, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

17140671

Citation

Kumarasamy, V, et al. "Evaluation of a Commercial Dengue NS1 Antigen-capture ELISA for Laboratory Diagnosis of Acute Dengue Virus Infection." Journal of Virological Methods, vol. 140, no. 1-2, 2007, pp. 75-9.
Kumarasamy V, Wahab AH, Chua SK, et al. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods. 2007;140(1-2):75-9.
Kumarasamy, V., Wahab, A. H., Chua, S. K., Hassan, Z., Chem, Y. K., Mohamad, M., & Chua, K. B. (2007). Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. Journal of Virological Methods, 140(1-2), 75-9.
Kumarasamy V, et al. Evaluation of a Commercial Dengue NS1 Antigen-capture ELISA for Laboratory Diagnosis of Acute Dengue Virus Infection. J Virol Methods. 2007;140(1-2):75-9. PubMed PMID: 17140671.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. AU - Kumarasamy,V, AU - Wahab,A H Abdul, AU - Chua,S K, AU - Hassan,Z, AU - Chem,Y K, AU - Mohamad,M, AU - Chua,K B, Y1 - 2006/11/30/ PY - 2006/07/16/received PY - 2006/10/28/revised PY - 2006/11/02/accepted PY - 2006/12/5/pubmed PY - 2007/4/12/medline PY - 2006/12/5/entrez SP - 75 EP - 9 JF - Journal of virological methods JO - J Virol Methods VL - 140 IS - 1-2 N2 - A commercial dengue NS1 antigen-capture ELISA was evaluated to demonstrate its potential application for early laboratory diagnosis of acute dengue virus infection. Dengue virus NS1 antigen was detected in 199 of 213 acute serum samples from patients with laboratory confirmation of acute dengue virus infection but none of the 354 healthy blood donors' serum specimens. The dengue NS1 antigen-capture ELISA gave an overall sensitivity of 93.4% (199/213) and a specificity of 100% (354/354). The sensitivity was significantly higher in acute primary dengue (97.3%) than in acute secondary dengue (70.0%). The positive predictive value of the dengue NS1 antigen-capture ELISA was 100% and negative predictive value was 97.3%. Comparatively, virus isolation gave an overall positive isolation rate of 68.1% with a positive isolation rate of 73.9 and 31.0% for acute primary dengue and acute secondary dengue, respectively. Molecular detection of dengue RNA by RT-PCR gave an overall positive detection rate of 66.7% with a detection rate of 65.2 and 75.9% for acute primary dengue and acute secondary dengue, respectively. The results indicate that the commercial dengue NS1 antigen-capture ELISA may be superior to virus isolation and RT-PCR for the laboratory diagnosis of acute dengue infection based on a single serum sample. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/17140671/Evaluation_of_a_commercial_dengue_NS1_antigen_capture_ELISA_for_laboratory_diagnosis_of_acute_dengue_virus_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(06)00385-5 DB - PRIME DP - Unbound Medicine ER -