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Determination of propiverine and its metabolites in rat samples by liquid chromatography-tandem mass spectrometry.
J Chromatogr A. 2007 May 11; 1149(1):121-6.JC

Abstract

A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method was developed and validated for the determination of the anticholinergic and antimuscarinc drug propiverine and eight of its metabolites in serum, urine, faeces and different tissue samples of rats. Samples containing propiverine and its metabolites in serum and urine and in the supernatants of faeces and tissue homogenates were extracted and cleaned up using an automated solid phase extraction (SPE) method. An external calibration was used. The analytes were measured employing the multiple reaction monitoring mode (MRM). A sufficient response over the range of 10-1000 ng/ml was demonstrated. The lower limit of quantification of the nine substances was 10 ng/ml. The presented method is suitable for pharmacokinetic or toxicokinetic studies. To look for additional unknown metabolites, the LC-MS-MS system operated in the precursor ion mode using typical product ions of propiverine and of its metabolites. With the help of the chromatographic behaviour and typical fragment ions of the unknown metabolites, it was possible to elucidate their structure. Five until now unknown metabolites were found in the urine and faeces samples. However, without reference substances, a quantification of these analytes was not possible.

Authors+Show Affiliations

Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany. Reinhard.Oertel@tu-dresden.de <Reinhard.Oertel@tu-dresden.de>No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17141252

Citation

Oertel, R, et al. "Determination of Propiverine and Its Metabolites in Rat Samples By Liquid Chromatography-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1149, no. 1, 2007, pp. 121-6.
Oertel R, Kilian B, Siegmund W, et al. Determination of propiverine and its metabolites in rat samples by liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2007;1149(1):121-6.
Oertel, R., Kilian, B., Siegmund, W., & Kirch, W. (2007). Determination of propiverine and its metabolites in rat samples by liquid chromatography-tandem mass spectrometry. Journal of Chromatography. A, 1149(1), 121-6.
Oertel R, et al. Determination of Propiverine and Its Metabolites in Rat Samples By Liquid Chromatography-tandem Mass Spectrometry. J Chromatogr A. 2007 May 11;1149(1):121-6. PubMed PMID: 17141252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of propiverine and its metabolites in rat samples by liquid chromatography-tandem mass spectrometry. AU - Oertel,R, AU - Kilian,B, AU - Siegmund,W, AU - Kirch,W, Y1 - 2006/12/01/ PY - 2006/08/25/received PY - 2006/11/01/revised PY - 2006/11/03/accepted PY - 2006/12/5/pubmed PY - 2007/7/28/medline PY - 2006/12/5/entrez SP - 121 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1149 IS - 1 N2 - A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method was developed and validated for the determination of the anticholinergic and antimuscarinc drug propiverine and eight of its metabolites in serum, urine, faeces and different tissue samples of rats. Samples containing propiverine and its metabolites in serum and urine and in the supernatants of faeces and tissue homogenates were extracted and cleaned up using an automated solid phase extraction (SPE) method. An external calibration was used. The analytes were measured employing the multiple reaction monitoring mode (MRM). A sufficient response over the range of 10-1000 ng/ml was demonstrated. The lower limit of quantification of the nine substances was 10 ng/ml. The presented method is suitable for pharmacokinetic or toxicokinetic studies. To look for additional unknown metabolites, the LC-MS-MS system operated in the precursor ion mode using typical product ions of propiverine and of its metabolites. With the help of the chromatographic behaviour and typical fragment ions of the unknown metabolites, it was possible to elucidate their structure. Five until now unknown metabolites were found in the urine and faeces samples. However, without reference substances, a quantification of these analytes was not possible. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17141252/Determination_of_propiverine_and_its_metabolites_in_rat_samples_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)02104-2 DB - PRIME DP - Unbound Medicine ER -