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Bioavailability and toxicity of pentachlorophenol in contaminated soil evaluated on coelomocytes of Eisenia andrei (Annelida: Lumbricidae).
Toxicol In Vitro. 2007 Mar; 21(2):302-7.TV

Abstract

Pentachlorophenol (PCP) is widely distributed and highly persistent in soil, and represents a threat to the health of ecosystems. The present study aimed to assess the toxicity and bioavailability of PCP in soils as a function of different aging periods with the attempt to select a good toxicological assay for Eisenia andrei Bouché (Annelida: Lumbricidae). The experiments were performed on soil contaminated with PCP at 15 and 150ppm. After different aging periods (20, 60 and 120 days from spiking), bioavailability and toxicity were evaluated on E. andrei kept for 7 and 14 days in treated soils. The actual bioavailability decreased in relation to the aging for both PCP concentrations. No membrane damage was observed on coelomocytes collected by ethanol extrusion. Modifications in distribution of coelomocyte subpopulations were detected by flow cytometry on samples aged for 60 and 120 days at 150ppm PCP contamination. The reduction of lysosomal membrane stability, measured by neutral red retention time, was observed in all treatments. Worm mortality increased with aging in soils spiked with 150ppm of PCP. In conclusion, aging did not seem to reduce PCP cytotoxicity. This is the first report on in vivo toxicity of PCP evaluated on coelomocytes of E. andrei using different assays.

Authors+Show Affiliations

Department of Agricultural and Environmental Biology and Chemistry, University of Bari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17142006

Citation

Vernile, Pasqua, et al. "Bioavailability and Toxicity of Pentachlorophenol in Contaminated Soil Evaluated On Coelomocytes of Eisenia Andrei (Annelida: Lumbricidae)." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 21, no. 2, 2007, pp. 302-7.
Vernile P, Fornelli F, Bari G, et al. Bioavailability and toxicity of pentachlorophenol in contaminated soil evaluated on coelomocytes of Eisenia andrei (Annelida: Lumbricidae). Toxicol In Vitro. 2007;21(2):302-7.
Vernile, P., Fornelli, F., Bari, G., Spagnuolo, M., Minervini, F., de Lillo, E., & Ruggiero, P. (2007). Bioavailability and toxicity of pentachlorophenol in contaminated soil evaluated on coelomocytes of Eisenia andrei (Annelida: Lumbricidae). Toxicology in Vitro : an International Journal Published in Association With BIBRA, 21(2), 302-7.
Vernile P, et al. Bioavailability and Toxicity of Pentachlorophenol in Contaminated Soil Evaluated On Coelomocytes of Eisenia Andrei (Annelida: Lumbricidae). Toxicol In Vitro. 2007;21(2):302-7. PubMed PMID: 17142006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioavailability and toxicity of pentachlorophenol in contaminated soil evaluated on coelomocytes of Eisenia andrei (Annelida: Lumbricidae). AU - Vernile,Pasqua, AU - Fornelli,Francesca, AU - Bari,Giuseppe, AU - Spagnuolo,Matteo, AU - Minervini,Fiorenza, AU - de Lillo,Enrico, AU - Ruggiero,Pacifico, Y1 - 2006/10/10/ PY - 2006/04/07/received PY - 2006/09/19/revised PY - 2006/09/29/accepted PY - 2006/12/5/pubmed PY - 2009/7/22/medline PY - 2006/12/5/entrez SP - 302 EP - 7 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 21 IS - 2 N2 - Pentachlorophenol (PCP) is widely distributed and highly persistent in soil, and represents a threat to the health of ecosystems. The present study aimed to assess the toxicity and bioavailability of PCP in soils as a function of different aging periods with the attempt to select a good toxicological assay for Eisenia andrei Bouché (Annelida: Lumbricidae). The experiments were performed on soil contaminated with PCP at 15 and 150ppm. After different aging periods (20, 60 and 120 days from spiking), bioavailability and toxicity were evaluated on E. andrei kept for 7 and 14 days in treated soils. The actual bioavailability decreased in relation to the aging for both PCP concentrations. No membrane damage was observed on coelomocytes collected by ethanol extrusion. Modifications in distribution of coelomocyte subpopulations were detected by flow cytometry on samples aged for 60 and 120 days at 150ppm PCP contamination. The reduction of lysosomal membrane stability, measured by neutral red retention time, was observed in all treatments. Worm mortality increased with aging in soils spiked with 150ppm of PCP. In conclusion, aging did not seem to reduce PCP cytotoxicity. This is the first report on in vivo toxicity of PCP evaluated on coelomocytes of E. andrei using different assays. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/17142006/Bioavailability_and_toxicity_of_pentachlorophenol_in_contaminated_soil_evaluated_on_coelomocytes_of_Eisenia_andrei__Annelida:_Lumbricidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(06)00226-8 DB - PRIME DP - Unbound Medicine ER -