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Greater diversity of Shiga toxin-encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans.
Appl Environ Microbiol. 2007 Feb; 73(3):671-9.AE

Abstract

Escherichia coli O157:H7, a zoonotic human pathogen for which domestic cattle are a reservoir host, produces a Shiga toxin(s) (Stx) encoded by bacteriophages. Chromosomal insertion sites of these bacteriophages define three principal genotypes (clusters 1 to 3) among clinical isolates of E. coli O157:H7. Stx-encoding bacteriophage insertion site genotypes of 282 clinical and 80 bovine isolates were evaluated. A total of 268 (95.0%) of the clinical isolates, but only 41 (51.3%) of the bovine isolates, belonged to cluster 1, 2, or 3 (P < 0.001). Thirteen additional genotypes were identified in isolates from both cattle and humans (four genotypes), from only cattle (seven genotypes), or from only humans (two genotypes). Two other markers previously associated with isolates from cattle or with clinical isolates showed similar associations with genotype groups within bovine isolates; the tir allele sp-1 and the Q933W allele were under- and overrepresented, respectively, among cluster 1 to 3 genotypes. Stx-encoding bacteriophage insertion site typing demonstrated that there is broad genetic diversity of E. coli O157:H7 in the bovine reservoir and that numerous genotypes are significantly underrepresented among clinical isolates, consistent with the possibility that there is reduced virulence or transmissibility to humans of some bovine E. coli O157:H7 genotypes.

Authors+Show Affiliations

Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA. tbesser@vetmed.wsu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17142358

Citation

Besser, Thomas E., et al. "Greater Diversity of Shiga Toxin-encoding Bacteriophage Insertion Sites Among Escherichia Coli O157:H7 Isolates From Cattle Than in Those From Humans." Applied and Environmental Microbiology, vol. 73, no. 3, 2007, pp. 671-9.
Besser TE, Shaikh N, Holt NJ, et al. Greater diversity of Shiga toxin-encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans. Appl Environ Microbiol. 2007;73(3):671-9.
Besser, T. E., Shaikh, N., Holt, N. J., Tarr, P. I., Konkel, M. E., Malik-Kale, P., Walsh, C. W., Whittam, T. S., & Bono, J. L. (2007). Greater diversity of Shiga toxin-encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans. Applied and Environmental Microbiology, 73(3), 671-9.
Besser TE, et al. Greater Diversity of Shiga Toxin-encoding Bacteriophage Insertion Sites Among Escherichia Coli O157:H7 Isolates From Cattle Than in Those From Humans. Appl Environ Microbiol. 2007;73(3):671-9. PubMed PMID: 17142358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Greater diversity of Shiga toxin-encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans. AU - Besser,Thomas E, AU - Shaikh,Nurmohammad, AU - Holt,Nicholas J, AU - Tarr,Phillip I, AU - Konkel,Michael E, AU - Malik-Kale,Preeti, AU - Walsh,Coilin W, AU - Whittam,Thomas S, AU - Bono,James L, Y1 - 2006/12/01/ PY - 2006/12/5/pubmed PY - 2007/3/28/medline PY - 2006/12/5/entrez SP - 671 EP - 9 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 73 IS - 3 N2 - Escherichia coli O157:H7, a zoonotic human pathogen for which domestic cattle are a reservoir host, produces a Shiga toxin(s) (Stx) encoded by bacteriophages. Chromosomal insertion sites of these bacteriophages define three principal genotypes (clusters 1 to 3) among clinical isolates of E. coli O157:H7. Stx-encoding bacteriophage insertion site genotypes of 282 clinical and 80 bovine isolates were evaluated. A total of 268 (95.0%) of the clinical isolates, but only 41 (51.3%) of the bovine isolates, belonged to cluster 1, 2, or 3 (P < 0.001). Thirteen additional genotypes were identified in isolates from both cattle and humans (four genotypes), from only cattle (seven genotypes), or from only humans (two genotypes). Two other markers previously associated with isolates from cattle or with clinical isolates showed similar associations with genotype groups within bovine isolates; the tir allele sp-1 and the Q933W allele were under- and overrepresented, respectively, among cluster 1 to 3 genotypes. Stx-encoding bacteriophage insertion site typing demonstrated that there is broad genetic diversity of E. coli O157:H7 in the bovine reservoir and that numerous genotypes are significantly underrepresented among clinical isolates, consistent with the possibility that there is reduced virulence or transmissibility to humans of some bovine E. coli O157:H7 genotypes. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/17142358/Greater_diversity_of_Shiga_toxin_encoding_bacteriophage_insertion_sites_among_Escherichia_coli_O157:H7_isolates_from_cattle_than_in_those_from_humans_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&amp;pmid=17142358 DB - PRIME DP - Unbound Medicine ER -