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Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair.
Mol Cell Biol. 2007 Feb; 27(4):1191-206.MC

Abstract

The eukaryal Snu13p/15.5K protein binds K-turn motifs in U4 snRNA and snoRNAs. Two Snu13p/15.5K molecules bind the nucleolar U3 snoRNA required for the early steps of preribosomal processing. Binding of one molecule on the C'/D motif allows association of proteins Nop1p, Nop56p, and Nop58p, whereas binding of the second molecule on the B/C motif allows Rrp9p recruitment. To understand how the Snu13p-Rrp9p pair recognizes the B/C motif, we first improved the identification of RNA determinants required for Snu13p binding by experiments using the systematic evolution of ligands by exponential enrichment. This demonstrated the importance of a U.U pair stacked on the sheared pairs and revealed a direct link between Snu13p affinity and the stability of helices I and II. Sequence and structure requirements for efficient association of Rrp9p on the B/C motif were studied in yeast cells by expression of variant U3 snoRNAs and immunoselection assays. A G-C pair in stem II, a G residue at position 1 in the bulge, and a short stem I were found to be required. The data identify the in vivo function of most of the conserved residues of the U3 snoRNA B/C motif. They bring important information to understand how different K-turn motifs can recruit different sets of proteins after Snu13p association.

Authors+Show Affiliations

Laboratoire de Maturation des ARN et Enzymologie Moléculaire, UMR 7567, Université Henri Poincaré, Nancy I, BP 239, 54506 Vandoeuvre-lès-Nancy, France. christiane.branlant@maem.uhp-nancy.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17145781

Citation

Cléry, A, et al. "Analysis of Sequence and Structural Features That Identify the B/C Motif of U3 Small Nucleolar RNA as the Recognition Site for the Snu13p-Rrp9p Protein Pair." Molecular and Cellular Biology, vol. 27, no. 4, 2007, pp. 1191-206.
Cléry A, Senty-Ségault V, Leclerc F, et al. Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair. Mol Cell Biol. 2007;27(4):1191-206.
Cléry, A., Senty-Ségault, V., Leclerc, F., Raué, H. A., & Branlant, C. (2007). Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair. Molecular and Cellular Biology, 27(4), 1191-206.
Cléry A, et al. Analysis of Sequence and Structural Features That Identify the B/C Motif of U3 Small Nucleolar RNA as the Recognition Site for the Snu13p-Rrp9p Protein Pair. Mol Cell Biol. 2007;27(4):1191-206. PubMed PMID: 17145781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair. AU - Cléry,A, AU - Senty-Ségault,V, AU - Leclerc,F, AU - Raué,H A, AU - Branlant,C, Y1 - 2006/12/04/ PY - 2006/12/6/pubmed PY - 2007/3/17/medline PY - 2006/12/6/entrez SP - 1191 EP - 206 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 27 IS - 4 N2 - The eukaryal Snu13p/15.5K protein binds K-turn motifs in U4 snRNA and snoRNAs. Two Snu13p/15.5K molecules bind the nucleolar U3 snoRNA required for the early steps of preribosomal processing. Binding of one molecule on the C'/D motif allows association of proteins Nop1p, Nop56p, and Nop58p, whereas binding of the second molecule on the B/C motif allows Rrp9p recruitment. To understand how the Snu13p-Rrp9p pair recognizes the B/C motif, we first improved the identification of RNA determinants required for Snu13p binding by experiments using the systematic evolution of ligands by exponential enrichment. This demonstrated the importance of a U.U pair stacked on the sheared pairs and revealed a direct link between Snu13p affinity and the stability of helices I and II. Sequence and structure requirements for efficient association of Rrp9p on the B/C motif were studied in yeast cells by expression of variant U3 snoRNAs and immunoselection assays. A G-C pair in stem II, a G residue at position 1 in the bulge, and a short stem I were found to be required. The data identify the in vivo function of most of the conserved residues of the U3 snoRNA B/C motif. They bring important information to understand how different K-turn motifs can recruit different sets of proteins after Snu13p association. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/17145781/Analysis_of_sequence_and_structural_features_that_identify_the_B/C_motif_of_U3_small_nucleolar_RNA_as_the_recognition_site_for_the_Snu13p_Rrp9p_protein_pair_ L2 - http://mcb.asm.org/cgi/pmidlookup?view=long&pmid=17145781 DB - PRIME DP - Unbound Medicine ER -