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Participation of both host and virus factors in induction of severe acute respiratory syndrome (SARS) in F344 rats infected with SARS coronavirus.
J Virol. 2007 Feb; 81(4):1848-57.JV

Abstract

To understand the pathogenesis and develop an animal model of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), the Frankfurt 1 SARS-CoV isolate was passaged serially in young F344 rats. Young rats were susceptible to SARS-CoV but cleared the virus rapidly within 3 to 5 days of intranasal inoculation. After 10 serial passages, replication and virulence of SARS-CoV were increased in the respiratory tract of young rats without clinical signs. By contrast, adult rats infected with the passaged virus showed respiratory symptoms and severe pathological lesions in the lung. Levels of inflammatory cytokines in sera and lung tissues were significantly higher in adult F344 rats than in young rats. During in vivo passage of SARS-CoV, a single amino acid substitution was introduced within the binding domain of the viral spike protein recognizing angiotensin-converting enzyme 2 (ACE2), which is known as a SARS-CoV receptor. The rat-passaged virus more efficiently infected CHO cells expressing rat ACE2 than did the original isolate. These results strongly indicate that host and virus factors such as advanced age and virus adaptation are critical for the development of SARS in rats.

Authors+Show Affiliations

Department of Pathology, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan. nnagata@nih.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17151094

Citation

Nagata, Noriyo, et al. "Participation of Both Host and Virus Factors in Induction of Severe Acute Respiratory Syndrome (SARS) in F344 Rats Infected With SARS Coronavirus." Journal of Virology, vol. 81, no. 4, 2007, pp. 1848-57.
Nagata N, Iwata N, Hasegawa H, et al. Participation of both host and virus factors in induction of severe acute respiratory syndrome (SARS) in F344 rats infected with SARS coronavirus. J Virol. 2007;81(4):1848-57.
Nagata, N., Iwata, N., Hasegawa, H., Fukushi, S., Yokoyama, M., Harashima, A., Sato, Y., Saijo, M., Morikawa, S., & Sata, T. (2007). Participation of both host and virus factors in induction of severe acute respiratory syndrome (SARS) in F344 rats infected with SARS coronavirus. Journal of Virology, 81(4), 1848-57.
Nagata N, et al. Participation of Both Host and Virus Factors in Induction of Severe Acute Respiratory Syndrome (SARS) in F344 Rats Infected With SARS Coronavirus. J Virol. 2007;81(4):1848-57. PubMed PMID: 17151094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Participation of both host and virus factors in induction of severe acute respiratory syndrome (SARS) in F344 rats infected with SARS coronavirus. AU - Nagata,Noriyo, AU - Iwata,Naoko, AU - Hasegawa,Hideki, AU - Fukushi,Shuetsu, AU - Yokoyama,Masaru, AU - Harashima,Ayako, AU - Sato,Yuko, AU - Saijo,Masayuki, AU - Morikawa,Shigeru, AU - Sata,Tetsutaro, Y1 - 2006/12/06/ PY - 2006/12/8/pubmed PY - 2007/3/21/medline PY - 2006/12/8/entrez SP - 1848 EP - 57 JF - Journal of virology JO - J Virol VL - 81 IS - 4 N2 - To understand the pathogenesis and develop an animal model of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), the Frankfurt 1 SARS-CoV isolate was passaged serially in young F344 rats. Young rats were susceptible to SARS-CoV but cleared the virus rapidly within 3 to 5 days of intranasal inoculation. After 10 serial passages, replication and virulence of SARS-CoV were increased in the respiratory tract of young rats without clinical signs. By contrast, adult rats infected with the passaged virus showed respiratory symptoms and severe pathological lesions in the lung. Levels of inflammatory cytokines in sera and lung tissues were significantly higher in adult F344 rats than in young rats. During in vivo passage of SARS-CoV, a single amino acid substitution was introduced within the binding domain of the viral spike protein recognizing angiotensin-converting enzyme 2 (ACE2), which is known as a SARS-CoV receptor. The rat-passaged virus more efficiently infected CHO cells expressing rat ACE2 than did the original isolate. These results strongly indicate that host and virus factors such as advanced age and virus adaptation are critical for the development of SARS in rats. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/17151094/Participation_of_both_host_and_virus_factors_in_induction_of_severe_acute_respiratory_syndrome__SARS__in_F344_rats_infected_with_SARS_coronavirus_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=17151094 DB - PRIME DP - Unbound Medicine ER -