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A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors.
Biotechnol Lett 2007; 29(3):431-7BL

Abstract

A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an L-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system.

Authors+Show Affiliations

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17151958

Citation

Zhang, Huijing, et al. "A Novel High-copy Plasmid, pEC, Compatible With Commonly Used Escherichia Coli Cloning and Expression Vectors." Biotechnology Letters, vol. 29, no. 3, 2007, pp. 431-7.
Zhang H, Wu H, Zhang H. A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors. Biotechnol Lett. 2007;29(3):431-7.
Zhang, H., Wu, H., & Zhang, H. (2007). A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors. Biotechnology Letters, 29(3), pp. 431-7.
Zhang H, Wu H, Zhang H. A Novel High-copy Plasmid, pEC, Compatible With Commonly Used Escherichia Coli Cloning and Expression Vectors. Biotechnol Lett. 2007;29(3):431-7. PubMed PMID: 17151958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors. AU - Zhang,Huijing, AU - Wu,Haizhen, AU - Zhang,Huizhan, Y1 - 2006/12/07/ PY - 2006/08/02/received PY - 2006/10/30/accepted PY - 2006/10/27/revised PY - 2006/12/8/pubmed PY - 2007/9/14/medline PY - 2006/12/8/entrez SP - 431 EP - 7 JF - Biotechnology letters JO - Biotechnol. Lett. VL - 29 IS - 3 N2 - A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an L-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/17151958/A_novel_high_copy_plasmid_pEC_compatible_with_commonly_used_Escherichia_coli_cloning_and_expression_vectors_ L2 - https://doi.org/10.1007/s10529-006-9258-5 DB - PRIME DP - Unbound Medicine ER -