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Encystment and metacercariae development of Echinostoma cinetorchis cercariae in an in vitro culture system.
J Parasitol. 2006 Oct; 92(5):1010-3.JP

Abstract

For some species of 37-collar-spined Echinostoma, their cercariae successfully encyst and develop to metacercariae in vitro. In our study, we cultured Echinostoma cinetorchis cercariae in 12 different media to study the formation of metacercariae. Locke's solution, medium 199, and RPMI 1640 were used as media for culture. RPMI 1640 produced the highest encystment and normal metacercariae development. The osmolality of the media was related to their ability to encyst and develop. The 0.5 X media induced higher encystment and normal metacercaria formation than the 1x media. The addition of fetal bovine serum to RPMI 1640 increased the level of encystment and normal metacercariae development. In the mixture of 0.5 x RPMI 1640 and 10% fetal bovine serum, encystment was highest, at 96.0%, and the development ratio of normal metacercariae was also the highest, at 91.5%, after 48 hr of cultivation. In a viability test, 7 day- and 14 day-cultured metacercariae were successfully matured to adult worms in experimentally infected rats. These results showed that E. cinetorchis cercariae could be cultured to viable metacercariae in an in vitro culture system and that 0.5 x RPMI 1640 plus 10% fetal bovine serum was the most useful medium for cultivation. This culture system can be adapted for additional studies on the E. cinetorchis life cycle, especially to supply large numbers of metacercariae for other studies on this echinostome.

Authors+Show Affiliations

Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon 400-712, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17152942

Citation

Park, Yun-Kyu, et al. "Encystment and Metacercariae Development of Echinostoma Cinetorchis Cercariae in an in Vitro Culture System." The Journal of Parasitology, vol. 92, no. 5, 2006, pp. 1010-3.
Park YK, Hwang MK, Chung PR. Encystment and metacercariae development of Echinostoma cinetorchis cercariae in an in vitro culture system. J Parasitol. 2006;92(5):1010-3.
Park, Y. K., Hwang, M. K., & Chung, P. R. (2006). Encystment and metacercariae development of Echinostoma cinetorchis cercariae in an in vitro culture system. The Journal of Parasitology, 92(5), 1010-3.
Park YK, Hwang MK, Chung PR. Encystment and Metacercariae Development of Echinostoma Cinetorchis Cercariae in an in Vitro Culture System. J Parasitol. 2006;92(5):1010-3. PubMed PMID: 17152942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Encystment and metacercariae development of Echinostoma cinetorchis cercariae in an in vitro culture system. AU - Park,Yun-Kyu, AU - Hwang,Myung-Ki, AU - Chung,Pyung-Rim, PY - 2006/12/13/pubmed PY - 2006/12/23/medline PY - 2006/12/13/entrez SP - 1010 EP - 3 JF - The Journal of parasitology JO - J. Parasitol. VL - 92 IS - 5 N2 - For some species of 37-collar-spined Echinostoma, their cercariae successfully encyst and develop to metacercariae in vitro. In our study, we cultured Echinostoma cinetorchis cercariae in 12 different media to study the formation of metacercariae. Locke's solution, medium 199, and RPMI 1640 were used as media for culture. RPMI 1640 produced the highest encystment and normal metacercariae development. The osmolality of the media was related to their ability to encyst and develop. The 0.5 X media induced higher encystment and normal metacercaria formation than the 1x media. The addition of fetal bovine serum to RPMI 1640 increased the level of encystment and normal metacercariae development. In the mixture of 0.5 x RPMI 1640 and 10% fetal bovine serum, encystment was highest, at 96.0%, and the development ratio of normal metacercariae was also the highest, at 91.5%, after 48 hr of cultivation. In a viability test, 7 day- and 14 day-cultured metacercariae were successfully matured to adult worms in experimentally infected rats. These results showed that E. cinetorchis cercariae could be cultured to viable metacercariae in an in vitro culture system and that 0.5 x RPMI 1640 plus 10% fetal bovine serum was the most useful medium for cultivation. This culture system can be adapted for additional studies on the E. cinetorchis life cycle, especially to supply large numbers of metacercariae for other studies on this echinostome. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/17152942/Encystment_and_metacercariae_development_of_Echinostoma_cinetorchis_cercariae_in_an_in_vitro_culture_system_ L2 - http://www.journalofparasitology.org/doi/10.1645/GE-794R.1?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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