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Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps.
Allergy. 2007 Jan; 62(1):66-72.A

Abstract

BACKGROUND

Nasal polyps (NP), a subgroup of chronic rhinosinusitis, are characterized by interleukin 5 (IL-5) mediated infiltration of eosinophils in sinus mucosa, leading to pseudostratified ciliated columnar epithelium, thickening of the epithelial basement membrane and tissue edema. Matrix metalloproteinases (MMP) constitute a large group of Zn2+ dependent endopeptidases with the ability to degrade extracellular matrix and are possibly responsible for the development of tissue edema in chronic sinusitis.

OBJECTIVE

The aim of this study was to determine the expression of MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) mRNA and to locate the distribution of MMP-2, MMP-9 and TIMP-1 by immunohistochemistry in ethmoid sinus mucosa in NP. Furthermore the correlation between IL-5 or IL-8 and MMP-2, MMP-9 or TIMP-1 is examined.

METHODS

Nasal polyps of 33 patients and 18 specimens of inferior turbinate mucosa were examined by real time RT-PCR for MMP-2, MMP-9, TIMP-1, IL-5 and IL-8 mRNA expression. Immunohistochemical labeling for MMP-2, MMP-9 and TIMP-1 was performed.

RESULTS

Differences between both locations were detectable for MMP-9 (P < 0.001) and IL-5 (P=0.003) but not for MMP-2 (P=0.278), TIMP-1 (P=0.515) and IL-8 (P=0.386). Correlation was detected only between TIMP-1 and IL-5 (r=0.422, P =0.014). Cytoplasmic staining of MMP-2 was present in the apical part of the ciliated cells, submucosal glands and in smooth muscle cells. Matrix metalloproteinase-9 was expressed in surface epithelium, in seromucous glands and in polymorphonuclear cells.

CONCLUSIONS

Expression of MMP-9 and IL-5 mRNA are associated with NP. The correlation between IL-5 and TIMP-1 indicates the role of TIMP-1 in maintaining the homeostasis in NP.

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Authors+Show Affiliations

Chen YS
Department of Otorhinolaryngology, Plastic Head and Neck Surgery, University Hospital RWTH Aachen, Germany.
Langhammer T
No affiliation info available
Westhofen M
No affiliation info available
Lorenzen J
No affiliation info available

MeSH

AdultAgedFemaleGene ExpressionHumansImmunohistochemistryInterleukin-5Interleukin-8MaleMatrix Metalloproteinase 2Matrix Metalloproteinase 9Middle AgedNasal PolypsTissue DistributionTissue Inhibitor of Metalloproteinase-1

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17156344

Citation

Chen, Y-S, et al. "Relationship Between Matrix Metalloproteinases MMP-2, MMP-9, Tissue Inhibitor of Matrix Metalloproteinases-1 and IL-5, IL-8 in Nasal Polyps." Allergy, vol. 62, no. 1, 2007, pp. 66-72.
Chen YS, Langhammer T, Westhofen M, et al. Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps. Allergy. 2007;62(1):66-72.
Chen, Y. S., Langhammer, T., Westhofen, M., & Lorenzen, J. (2007). Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps. Allergy, 62(1), 66-72.
Chen YS, et al. Relationship Between Matrix Metalloproteinases MMP-2, MMP-9, Tissue Inhibitor of Matrix Metalloproteinases-1 and IL-5, IL-8 in Nasal Polyps. Allergy. 2007;62(1):66-72. PubMed PMID: 17156344.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps. AU - Chen,Y-S, AU - Langhammer,T, AU - Westhofen,M, AU - Lorenzen,J, PY - 2006/12/13/pubmed PY - 2007/5/9/medline PY - 2006/12/13/entrez SP - 66 EP - 72 JF - Allergy JO - Allergy VL - 62 IS - 1 N2 - BACKGROUND: Nasal polyps (NP), a subgroup of chronic rhinosinusitis, are characterized by interleukin 5 (IL-5) mediated infiltration of eosinophils in sinus mucosa, leading to pseudostratified ciliated columnar epithelium, thickening of the epithelial basement membrane and tissue edema. Matrix metalloproteinases (MMP) constitute a large group of Zn2+ dependent endopeptidases with the ability to degrade extracellular matrix and are possibly responsible for the development of tissue edema in chronic sinusitis. OBJECTIVE: The aim of this study was to determine the expression of MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) mRNA and to locate the distribution of MMP-2, MMP-9 and TIMP-1 by immunohistochemistry in ethmoid sinus mucosa in NP. Furthermore the correlation between IL-5 or IL-8 and MMP-2, MMP-9 or TIMP-1 is examined. METHODS: Nasal polyps of 33 patients and 18 specimens of inferior turbinate mucosa were examined by real time RT-PCR for MMP-2, MMP-9, TIMP-1, IL-5 and IL-8 mRNA expression. Immunohistochemical labeling for MMP-2, MMP-9 and TIMP-1 was performed. RESULTS: Differences between both locations were detectable for MMP-9 (P < 0.001) and IL-5 (P=0.003) but not for MMP-2 (P=0.278), TIMP-1 (P=0.515) and IL-8 (P=0.386). Correlation was detected only between TIMP-1 and IL-5 (r=0.422, P =0.014). Cytoplasmic staining of MMP-2 was present in the apical part of the ciliated cells, submucosal glands and in smooth muscle cells. Matrix metalloproteinase-9 was expressed in surface epithelium, in seromucous glands and in polymorphonuclear cells. CONCLUSIONS: Expression of MMP-9 and IL-5 mRNA are associated with NP. The correlation between IL-5 and TIMP-1 indicates the role of TIMP-1 in maintaining the homeostasis in NP. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/17156344/Relationship_between_matrix_metalloproteinases_MMP_2_MMP_9_tissue_inhibitor_of_matrix_metalloproteinases_1_and_IL_5_IL_8_in_nasal_polyps_ L2 - https://doi.org/10.1111/j.1398-9995.2006.01255.x DB - PRIME DP - Unbound Medicine ER -
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