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[Correlation of hepatitis B e antigen with hepatitis B virus DNA in the serum of chronic hepatitis B patients after treatment].
Zhonghua Yi Xue Za Zhi. 2006 Sep 05; 86(33):2348-51.ZY

Abstract

OBJECTIVE

To analyze the correlation of hepatitis B virus (HBV) DNA with the serological markers of HB in the serum of chronic HB patients after treatment PCR method and to analyze the status of these markers and the multiplication of virus.

METHODS

Peripheral blood samples were collected from 480 chronic HB patients, aged 15 - 50, who had been treated by anti-nucleotide drugs or traditional Chinese herbs and showed normal ALT/AST. Both COBAS AMPLICOR HBV MONIORTM kit (internal-standard PCR method) and Light Cycler real time fluorescent quantitative PCR instrument (external-quantitative standard PCR method) were used to measure the HBV DNAS level. 42 of the 312 patients with the HBV DNA level lower than the minimum test limit measured by COBAS AMPLICOR HBV MONIORTM kit and HBeAg positive (>4 S/CO) underwent microparticle enzyme immunoassay (MEIA) to test the HBsAg, anti-HBs, HBeAg, anti-HBe, and HBcIgG.

RESULTS

Seven of the 42 patients with HBV DNA negative measured by COBAS AMPLICOR HBV MONIORTM kit lower then the minimum test limit were shown as HBV DNA positive by Light Cycler real time fluorescent quantitative PCR. The 42 patients were HBsAg (+), anti-HBs (-), HBeAg (+), anti-HBe (-), and anti-HBcIgG (-), with an average HBeAg level of 42.26 S/CO and a positive HBeAg rate of 13.46%.

CONCLUSION

HBeAg positivity does not necessarily means an active multiplication of HBV. The changes of the serological markers of HBV may be not consistent with that of HBV DNA. It is more objective to undergo both internal-standard and external-quantitative standard methods.

Authors+Show Affiliations

Department of Clinical Lab, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17156634

Citation

Dou, Ya-ling, et al. "[Correlation of Hepatitis B E Antigen With Hepatitis B Virus DNA in the Serum of Chronic Hepatitis B Patients After Treatment]." Zhonghua Yi Xue Za Zhi, vol. 86, no. 33, 2006, pp. 2348-51.
Dou YL, Ni AP, Han JH, et al. [Correlation of hepatitis B e antigen with hepatitis B virus DNA in the serum of chronic hepatitis B patients after treatment]. Zhonghua Yi Xue Za Zhi. 2006;86(33):2348-51.
Dou, Y. L., Ni, A. P., Han, J. H., Sun, J. Y., & Yu, R. R. (2006). [Correlation of hepatitis B e antigen with hepatitis B virus DNA in the serum of chronic hepatitis B patients after treatment]. Zhonghua Yi Xue Za Zhi, 86(33), 2348-51.
Dou YL, et al. [Correlation of Hepatitis B E Antigen With Hepatitis B Virus DNA in the Serum of Chronic Hepatitis B Patients After Treatment]. Zhonghua Yi Xue Za Zhi. 2006 Sep 5;86(33):2348-51. PubMed PMID: 17156634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Correlation of hepatitis B e antigen with hepatitis B virus DNA in the serum of chronic hepatitis B patients after treatment]. AU - Dou,Ya-ling, AU - Ni,An-ping, AU - Han,Jian-hua, AU - Sun,Jiang-yan, AU - Yu,Rong-rong, PY - 2006/12/13/pubmed PY - 2008/4/15/medline PY - 2006/12/13/entrez SP - 2348 EP - 51 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 86 IS - 33 N2 - OBJECTIVE: To analyze the correlation of hepatitis B virus (HBV) DNA with the serological markers of HB in the serum of chronic HB patients after treatment PCR method and to analyze the status of these markers and the multiplication of virus. METHODS: Peripheral blood samples were collected from 480 chronic HB patients, aged 15 - 50, who had been treated by anti-nucleotide drugs or traditional Chinese herbs and showed normal ALT/AST. Both COBAS AMPLICOR HBV MONIORTM kit (internal-standard PCR method) and Light Cycler real time fluorescent quantitative PCR instrument (external-quantitative standard PCR method) were used to measure the HBV DNAS level. 42 of the 312 patients with the HBV DNA level lower than the minimum test limit measured by COBAS AMPLICOR HBV MONIORTM kit and HBeAg positive (>4 S/CO) underwent microparticle enzyme immunoassay (MEIA) to test the HBsAg, anti-HBs, HBeAg, anti-HBe, and HBcIgG. RESULTS: Seven of the 42 patients with HBV DNA negative measured by COBAS AMPLICOR HBV MONIORTM kit lower then the minimum test limit were shown as HBV DNA positive by Light Cycler real time fluorescent quantitative PCR. The 42 patients were HBsAg (+), anti-HBs (-), HBeAg (+), anti-HBe (-), and anti-HBcIgG (-), with an average HBeAg level of 42.26 S/CO and a positive HBeAg rate of 13.46%. CONCLUSION: HBeAg positivity does not necessarily means an active multiplication of HBV. The changes of the serological markers of HBV may be not consistent with that of HBV DNA. It is more objective to undergo both internal-standard and external-quantitative standard methods. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/17156634/[Correlation_of_hepatitis_B_e_antigen_with_hepatitis_B_virus_DNA_in_the_serum_of_chronic_hepatitis_B_patients_after_treatment]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2006&vol=86&issue=33&fpage=2348 DB - PRIME DP - Unbound Medicine ER -