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Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus.
Eur J Pharmacol. 2007 Feb 05; 556(1-3):157-65.EJ

Abstract

Transient receptor potential ion channel of the vanilloid type 1 (TRPV1)-dependent pathway, consisting of capsaicin-sensitive tachykininergic primary afferent and myenteric nitrergic neurons, has been suggested to mediate the inhibitory effect of capsaicin on vagally mediated striated muscle contractions in the rat esophagus. In a recent study, similar but also different effects of capsaicin and piperine on TRPV1 were demonstrated. Therefore, this study aimed to compare the effects of these two drugs on vagally induced contractions in the mouse esophagus. Capsaicin and piperine inhibited vagally induced contractions of a thoracic esophageal segment in a concentration-dependent manner. Ruthenium red (10 microM; a non-selective blocker of transient receptor potential cation channels) and SB-366791 (10 microM; a novel selective antagonist of TRPV1) blocked the inhibitory effect of capsaicin but not that of piperine. Piperine inhibited the vagally mediated contractions in esophagi of adult mice neonatally injected with capsaicin, while capsaicin failed to do so. Desensitization of TRPV1 in the mouse esophagus by in vitro pretreatment with capsaicin failed to affect the inhibitory effect of piperine, whereas the piperine effect was cross-desensitized by capsaicin pretreatment in rat and hamster esophagi. Additionally, a tachykinin NK(1) receptor antagonist, L-732,138 (1 microM), as well as a nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME 200 microM), blocked the inhibitory effect of capsaicin but not that of piperine. Taken together, the results suggest that piperine inhibits the vagally mediated striated muscle contraction in the mouse esophagus through its action on a TRPV1-dependent pathway as well as a TRPV1-independent site.

Authors+Show Affiliations

Department of Basic Veterinary Science, Laboratory of Physiology, The United Graduate School, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17156774

Citation

Boudaka, Ammar, et al. "Involvement of TRPV1-dependent and -independent Components in the Regulation of Vagally Induced Contractions in the Mouse Esophagus." European Journal of Pharmacology, vol. 556, no. 1-3, 2007, pp. 157-65.
Boudaka A, Wörl J, Shiina T, et al. Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus. Eur J Pharmacol. 2007;556(1-3):157-65.
Boudaka, A., Wörl, J., Shiina, T., Neuhuber, W. L., Kobayashi, H., Shimizu, Y., & Takewaki, T. (2007). Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus. European Journal of Pharmacology, 556(1-3), 157-65.
Boudaka A, et al. Involvement of TRPV1-dependent and -independent Components in the Regulation of Vagally Induced Contractions in the Mouse Esophagus. Eur J Pharmacol. 2007 Feb 5;556(1-3):157-65. PubMed PMID: 17156774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus. AU - Boudaka,Ammar, AU - Wörl,Jürgen, AU - Shiina,Takahiko, AU - Neuhuber,Winfried L, AU - Kobayashi,Haruo, AU - Shimizu,Yasutake, AU - Takewaki,Tadashi, Y1 - 2006/11/10/ PY - 2006/09/08/received PY - 2006/11/02/revised PY - 2006/11/06/accepted PY - 2006/12/13/pubmed PY - 2007/4/6/medline PY - 2006/12/13/entrez SP - 157 EP - 65 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 556 IS - 1-3 N2 - Transient receptor potential ion channel of the vanilloid type 1 (TRPV1)-dependent pathway, consisting of capsaicin-sensitive tachykininergic primary afferent and myenteric nitrergic neurons, has been suggested to mediate the inhibitory effect of capsaicin on vagally mediated striated muscle contractions in the rat esophagus. In a recent study, similar but also different effects of capsaicin and piperine on TRPV1 were demonstrated. Therefore, this study aimed to compare the effects of these two drugs on vagally induced contractions in the mouse esophagus. Capsaicin and piperine inhibited vagally induced contractions of a thoracic esophageal segment in a concentration-dependent manner. Ruthenium red (10 microM; a non-selective blocker of transient receptor potential cation channels) and SB-366791 (10 microM; a novel selective antagonist of TRPV1) blocked the inhibitory effect of capsaicin but not that of piperine. Piperine inhibited the vagally mediated contractions in esophagi of adult mice neonatally injected with capsaicin, while capsaicin failed to do so. Desensitization of TRPV1 in the mouse esophagus by in vitro pretreatment with capsaicin failed to affect the inhibitory effect of piperine, whereas the piperine effect was cross-desensitized by capsaicin pretreatment in rat and hamster esophagi. Additionally, a tachykinin NK(1) receptor antagonist, L-732,138 (1 microM), as well as a nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME 200 microM), blocked the inhibitory effect of capsaicin but not that of piperine. Taken together, the results suggest that piperine inhibits the vagally mediated striated muscle contraction in the mouse esophagus through its action on a TRPV1-dependent pathway as well as a TRPV1-independent site. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/17156774/Involvement_of_TRPV1_dependent_and__independent_components_in_the_regulation_of_vagally_induced_contractions_in_the_mouse_esophagus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(06)01258-1 DB - PRIME DP - Unbound Medicine ER -