Tags

Type your tag names separated by a space and hit enter

Changes in function of NMDA receptor NR2B subunit in spinal cord of rats with neuropathy following chronic ethanol consumption.
Life Sci. 2007 Feb 06; 80(9):852-9.LS

Abstract

Chronic ethanol consumption produces painful neuropathy for which there is no reliably successful therapy, largely due to a lack of understanding of the central mechanisms that underlie the development of the neuropathic pain-like state induced by chronic ethanol treatment. The aim of this study was to investigate what mechanisms contribute to the neuropathic pain-like state induced by chronic ethanol treatment in rats. Mechanical hyperalgesia was clearly observed during ethanol consumption and even after ethanol withdrawal, and lasted for 14 weeks. This hyperalgesia was significantly attenuated by repeated i.p. injection of ifenprodil, a selective NR2B subunit-containing NMDA receptor antagonist. Under these conditions, mRNA and protein levels of NR1, NR2A and NR2B subunits did not change in the spinal cord of chronic ethanol-fed rats. Interestingly, phosphorylated-Ser-1303 NR2B (p-Ser1303-NR2B) subunit was significantly increased in the spinal cord of chronic ethanol-fed rats, whereas p-Tyr1472-NR2B was not affected in the superficial spinal dorsal horn of ethanol-fed rats. These findings suggest that spinal p-Ser1303-NR2B plays a significant role in the development of the ethanol-dependent neuropathic pain-like state in rats.

Authors+Show Affiliations

Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. narita@hoshi.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17156796

Citation

Narita, Minoru, et al. "Changes in Function of NMDA Receptor NR2B Subunit in Spinal Cord of Rats With Neuropathy Following Chronic Ethanol Consumption." Life Sciences, vol. 80, no. 9, 2007, pp. 852-9.
Narita M, Miyoshi K, Narita M, et al. Changes in function of NMDA receptor NR2B subunit in spinal cord of rats with neuropathy following chronic ethanol consumption. Life Sci. 2007;80(9):852-9.
Narita, M., Miyoshi, K., Narita, M., & Suzuki, T. (2007). Changes in function of NMDA receptor NR2B subunit in spinal cord of rats with neuropathy following chronic ethanol consumption. Life Sciences, 80(9), 852-9.
Narita M, et al. Changes in Function of NMDA Receptor NR2B Subunit in Spinal Cord of Rats With Neuropathy Following Chronic Ethanol Consumption. Life Sci. 2007 Feb 6;80(9):852-9. PubMed PMID: 17156796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in function of NMDA receptor NR2B subunit in spinal cord of rats with neuropathy following chronic ethanol consumption. AU - Narita,Minoru, AU - Miyoshi,Kan, AU - Narita,Michiko, AU - Suzuki,Tsutomu, Y1 - 2006/11/15/ PY - 2006/09/10/received PY - 2006/10/25/revised PY - 2006/11/03/accepted PY - 2006/12/13/pubmed PY - 2007/3/16/medline PY - 2006/12/13/entrez SP - 852 EP - 9 JF - Life sciences JO - Life Sci VL - 80 IS - 9 N2 - Chronic ethanol consumption produces painful neuropathy for which there is no reliably successful therapy, largely due to a lack of understanding of the central mechanisms that underlie the development of the neuropathic pain-like state induced by chronic ethanol treatment. The aim of this study was to investigate what mechanisms contribute to the neuropathic pain-like state induced by chronic ethanol treatment in rats. Mechanical hyperalgesia was clearly observed during ethanol consumption and even after ethanol withdrawal, and lasted for 14 weeks. This hyperalgesia was significantly attenuated by repeated i.p. injection of ifenprodil, a selective NR2B subunit-containing NMDA receptor antagonist. Under these conditions, mRNA and protein levels of NR1, NR2A and NR2B subunits did not change in the spinal cord of chronic ethanol-fed rats. Interestingly, phosphorylated-Ser-1303 NR2B (p-Ser1303-NR2B) subunit was significantly increased in the spinal cord of chronic ethanol-fed rats, whereas p-Tyr1472-NR2B was not affected in the superficial spinal dorsal horn of ethanol-fed rats. These findings suggest that spinal p-Ser1303-NR2B plays a significant role in the development of the ethanol-dependent neuropathic pain-like state in rats. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/17156796/Changes_in_function_of_NMDA_receptor_NR2B_subunit_in_spinal_cord_of_rats_with_neuropathy_following_chronic_ethanol_consumption_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(06)00869-1 DB - PRIME DP - Unbound Medicine ER -