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Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells.
Blood. 2007 Apr 15; 109(8):3544-51.Blood

Abstract

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. In addition to red blood cells, Lu/BCAM proteins are highly expressed in endothelial cells. Abnormal adhesion of red blood cells to the endothelium could potentially contribute to the vaso-occlusive episodes in sickle cell disease. Considering the presence of integrin consensus-binding sites in Lu/BCAM proteins, we investigated their potential interaction with integrin alpha(4)beta(1), the unique integrin expressed on immature circulating sickle red cells. Using cell adhesion assays under static and flow conditions, we demonstrated that integrin alpha(4)beta(1) expressed on transfected cells bound to chimeric Lu-Fc protein. We showed that epinephrine-stimulated sickle cells, but not control red cells, adhered to Lu-Fc via integrin alpha(4)beta(1) under flow conditions. Antibody-mediated activation of integrin alpha(4)beta(1) induced adhesion of sickle red cells to primary human umbilical vein endothelial cells; this adhesion was inhibited by soluble Lu-Fc and vascular cell adhesion molecule-1 (VCAM-1)-Fc proteins. This novel interaction between integrin alpha(4)beta(1) in sickle red cells and endothelial Lu/BCAM proteins could participate in sickle cell adhesion to endothelium and potentially play a role in vaso-occlusive episodes.

Authors+Show Affiliations

INSERM Unité 665, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17158232

Citation

El Nemer, Wassim, et al. "Endothelial Lu/BCAM Glycoproteins Are Novel Ligands for Red Blood Cell Alpha4beta1 Integrin: Role in Adhesion of Sickle Red Blood Cells to Endothelial Cells." Blood, vol. 109, no. 8, 2007, pp. 3544-51.
El Nemer W, Wautier MP, Rahuel C, et al. Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. Blood. 2007;109(8):3544-51.
El Nemer, W., Wautier, M. P., Rahuel, C., Gane, P., Hermand, P., Galactéros, F., Wautier, J. L., Cartron, J. P., Colin, Y., & Le Van Kim, C. (2007). Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. Blood, 109(8), 3544-51.
El Nemer W, et al. Endothelial Lu/BCAM Glycoproteins Are Novel Ligands for Red Blood Cell Alpha4beta1 Integrin: Role in Adhesion of Sickle Red Blood Cells to Endothelial Cells. Blood. 2007 Apr 15;109(8):3544-51. PubMed PMID: 17158232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. AU - El Nemer,Wassim, AU - Wautier,Marie-Paule, AU - Rahuel,Cécile, AU - Gane,Pierre, AU - Hermand,Patricia, AU - Galactéros,Frédéric, AU - Wautier,Jean-Luc, AU - Cartron,Jean-Pierre, AU - Colin,Yves, AU - Le Van Kim,Caroline, Y1 - 2006/12/07/ PY - 2006/12/13/pubmed PY - 2007/5/24/medline PY - 2006/12/13/entrez SP - 3544 EP - 51 JF - Blood JO - Blood VL - 109 IS - 8 N2 - The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. In addition to red blood cells, Lu/BCAM proteins are highly expressed in endothelial cells. Abnormal adhesion of red blood cells to the endothelium could potentially contribute to the vaso-occlusive episodes in sickle cell disease. Considering the presence of integrin consensus-binding sites in Lu/BCAM proteins, we investigated their potential interaction with integrin alpha(4)beta(1), the unique integrin expressed on immature circulating sickle red cells. Using cell adhesion assays under static and flow conditions, we demonstrated that integrin alpha(4)beta(1) expressed on transfected cells bound to chimeric Lu-Fc protein. We showed that epinephrine-stimulated sickle cells, but not control red cells, adhered to Lu-Fc via integrin alpha(4)beta(1) under flow conditions. Antibody-mediated activation of integrin alpha(4)beta(1) induced adhesion of sickle red cells to primary human umbilical vein endothelial cells; this adhesion was inhibited by soluble Lu-Fc and vascular cell adhesion molecule-1 (VCAM-1)-Fc proteins. This novel interaction between integrin alpha(4)beta(1) in sickle red cells and endothelial Lu/BCAM proteins could participate in sickle cell adhesion to endothelium and potentially play a role in vaso-occlusive episodes. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/17158232/Endothelial_Lu/BCAM_glycoproteins_are_novel_ligands_for_red_blood_cell_alpha4beta1_integrin:_role_in_adhesion_of_sickle_red_blood_cells_to_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-4971(20)41735-5 DB - PRIME DP - Unbound Medicine ER -